NIDA Research Monograph, Number 70 [Printed in 1986]

Download Monograph70.pdf - Opioid Peptides: Molecular Pharmacology, Biosynthesis, and Analysis (4.6 MB)

Foreword-----v
Marvin Snyder

Introduction-----1
Rao S. Rapaka

Folding and Enzymatic Processing of Precursors of Biologically Active Peptides and Proteins-----3
Irwin M. Chaiken; Tatsuhiko Kanmera; and Reginald P. Sequeira

Biosynthesis of Opioid Peptides-----21
Olivier Civelli; Jim Douglass; Haim Rosen; Gerard Martens; and Edward Herbert

Proenkephalin Biosynthesis in the Rat-----43
Richard D. Howells

Enzymes in the Metabolism of Opioid Peptides: Isolation, Assay, and Specificity-----66
Neville Marks; Myron Benuck; and Martin J. Berg

Isolation and Identification of Opioid Peptides-----92
Hisayuki Matsuo

B-Endorphin: Naturally Occurring or Synthetic Agonists and Antagonists-----109
Choh Hao Li

Enkephalin Degrading Enzyme Inhibitors: A Physiological Way to New Analgesics and Psychoactive Agents-----128
Bernard P. Roques and Marie-Claude Fournie-Zaluski

Progress in the Characterization of the Opioid Receptor Subtypes: Peptides as Probes. Future Directions-----155
Eric J. Simon

Regulation of Agonist Binding to Opioid Receptor Types by Sodium and GTP: Relevance to Receptor Function-----175
Brian M. Cox; Linda L. Werling; and Gary Zarr

Opioid Receptors for the Dynorphin Peptides-----192
Iaian F. James

Computer Analysis of Radioligand Data: Advantages, Problems, and Pitfalls-----209
David Rodbard; Rudolph A. Lutz; Ricardo A. Cruciani; Vincenzo Guardabasso; Guido 0. Pesce; and Peter J. Munson

Recent Developments in Bioassay Using Selective Ligands and Selective In Vitro Preparations-----223
Hans W. Kosterlitz; Alistair D. Corbett; Maureen G. C. Gillan; Alexander T. McKnight; Stewart J. Paterson; and Linda E. Robson

Endorphins and Memory Regulation-----237
Ivan Izquierdo; Carlos A. Netto; and Renato D. Dias

Current Status of RIA Methods for the Analysis of Enkephalins and Endorphins-----255
R. Wayne Hendren

The Analysis of Endogenous Opioid Peptides With HPLC, Radioreceptor Assay, Radioimunoassay, and Mass Spectrometry-----304
Dominic M. Desiderio; Hisayoshi Takeshita; Hiroshi Onishi; Genevieve Fridland; Francis S. Tanzer; Claire Wakelyn; and Chhabil Dass

Reverse Phase HPLC of Peptides: Application to the Opioid Peptides-----319
Tatsuhiko Kanmera and Reginald P. Sequeira

Peptides as Drugs in the Treatment of Opiate Addiction-----337
Hemendra N. Bhargava

Progress in the Potential Use of Enkephalin Analogs-----367
Robert C. A. Frederickson

Opioid Peptides as Drug Products: FDA Regulatory Requirements-----385
Charles P. Hoiberg and Rao S. Rapaka

A Few Thoughts on the Development and Regulation of Neuropeptides-----405
John L. Gueriguian and Yuan-Yuan H. Chiu