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Overview
- Objectives
- Amplification
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Primer Design
- Primer Length
- Primer Melting Temperature
- Primer Annealing Temperature
- Values for Tm
- Maximum 3' Stability
- GC Content Percentage
- No Secondary Structures
- No self-complementarity
- No Complementarity to other primers
- No Long Runs With The Same Base
- Distance Between Two Primers On Target Sequence
- Plateau Effect
- Thermal Cyclers
- Thermal Cycling Parameters & Optimization
- Works Cited & Online Links
- Locus Selection
- Multiplexing
- Contamination
User Management
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