Technical Abstract: The ability to reliably analyze cellular and molecular profiles of normal or diseased tissues is frequently obfuscated by the inherent heterogeneous nature of tissues. Laser Capture Microdissection (LCM) is an innovative technique that allows the isolation and enrichment of pure subpopulations of cells from tissues under direct microscopic visualization. Material obtained from captured cells can be used for downstream assays, including gene expression studies such as microarrays, proteomic assays such as western blotting, cDNA library generation, and DNA genotyping. We describe a series of LCM protocols coupled to PREXCEL-Q-based qPCR for examining macrophage populations. We focus on two LCM approaches: ¿laser cutting¿ and ¿laser capture¿ for which the calculations for reagents and time involved have been pre-determined for 10 samples. One can expect the entire procedure for laser cutting coupled to qPCR to take approximately 12.5-15 h, and laser capture coupled to qPCR to take approximately 13.5-17.5 h.