Project Number: 5348-32000-028-03
Project Type: Grant

Start Date: Jul 01, 2004
End Date: Jun 30, 2009

Objective:
Complete genomic sequencing and annotation of the Babesia bovis genome.

Approach:
Our collaborative approach to obtaining the complete genomic sequence and annotation of B. bovis is as follows. The TBO (Texas) isolate of B. bovis was shown to be tick transmissible and able to cause clinical disease. Following tick transmission, cultures of TBO were established, amplified and high molecular weight DNA isolated. This DNA will be used to obtain shotgun sequence of B. bovis. Secondly, using the same DNA a BAC library (100X coverage) was established. The BAC library will be used to obtain BAC end sequences for genomic alignment and gap closure. Concurrently with our sequencing approach cDNAs will be obtained from infected Boophilus microplus midguts and salivary glands and the corresponding genes identified in the genomic sequence. These genes will be expressed and tested for their ability to induce transmission blocking immune responses. BSL-1; 6/30/05. Documents Grant with Institute of Genomic Research. Formerly 5348-32000-020-03G. (January 2007)