FIGURE 8.
Western blot of lysates expressing wild type or Arg to Cys mutant proteins after treatment with proteasome inhibitor, lactacystin (10 µM). NL3 (A), AChE-548 (B), and BChE (C). Me2SO (0.2%) was used to dissolve lactacystin. C, Me2SO-treated cells are included as a control. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and PTP-1B were used as loading controls. Lanes are labeled as follows: C, control; L, lactacystin; D, Me2SO (DMSO) treatment.