ARS | Publication request: Identification of Bovine Micrornas

Submitted to: Meeting Abstract
Publication Type: Abstract
Publication Acceptance Date: April 1, 2006
Publication Date: May 31, 2006
Citation: Matukumalli, L.K., Coutinho, L.L., Sonstegard, T.S., Van Tassell, C.P., Gasbarre, L.C., Capuco, A.V., Smith, T.P. 2006. Identification of bovine micrornas [abstract]. Cold Springs Harbor Laboratories 71 Symposium of Regulatoary RNAs. Cold Spring Harbor, NY. Number 214.

Technical Abstract: MicroRNAs are small ~22 nucleotides-long non-coding RNAs capable of controlling gene expression by inhibiting translation or targeting messenger RNA for degradation. Bovine genome sequence is not yet annotated for the microRNAs and there are currently no bovine miRNAs reported in the miRBase. Alignment of human microRNA sequences against the bovine genome resulted in the identification of 298 bovine miRs. In order to validate these predictions and identify new bovine microRNAs we have constructed and sequenced smallRNA cDNA libraries from bovine embryo, thymus, small intestine, abomasum, and mesenteric lymph nodes. This strategy allowed the identification of 130 bovine microRNAs. Of these, 101 aligned to known human microRNAs, 7 of them only aligned to the complementary strand of the mature microRNA in the database. The remaining 22 had secondary structures characteristic of microRNAs and 11 of these have phylogenetic conservation with other species.