Technical Abstract: Fusarium verticillioides (teleomorph Gibberella moniliformis) can be either an endophyte of maize, causing no visible disease, or a pathogen causing disease of ears, stalks, roots and seedlings. At any stage, this fungus can synthesize fumonisins, a family of mycotoxins structurally similar to the sphingolipid sphinganine. Ingestion of fumonisin-contaminated maize has been associated with a number of animal diseases, including cancer, and has been epidemiologically associated with human esophageal cancer in some regions of the world. A primary goal of our laboratory is to eliminate fumonisins contamination of maize and maize products. Understanding how and why these toxins are made and the F. verticillioides-maize disease process will allow us to develop novel strategies to limit tissue destruction (rot) and fumonisin production. To meet this goal, we are using genomic sequence data, Expressed Sequence Tags (ESTs), and microarrays to identify F. verticillioides genes involved in biosynthesis of toxins and plant pathogenesis. This report describes the current status of F. verticillioides genomic resources, and three approaches we are using to mine microarray data from a wild-type strain cultured in liquid fumonisin production medium for 12, 24, 48, 72, 96 and 120 hrs. Taken together, these approaches demonstrate the power of microarray technology to provide information on different biological processes.