Stephen R Hughes
Bioproducts and Biocatalysis Research
Research Molecular Biologist
Phone: (309) 681-6176
Fax: (309) 681-6040
Room 1106 NTL CENTER FOR AGRICULTURAL UTILIZATION RESEARCH
1815 N UNIVERSITY STREET PEORIA, IL, 61604
Rastogi, G., Muppidi, G., Gurram, R., Adhikari, A., Bang, S., Christopher, L., Bischoff, K.M., Hughes, S.R., Apel, W., Sani, R.K. 2009. Characterization of Extremophilic Cellulose-Degrading Bacteria from the Deep Subsurface of the Homestake Gold Mine, Lead, South Dakota [abstract]. Symposium on Biotechnology for Fuels and Chemicals.
Hughes, S.R. 2009. GMAX yeast background strain made from industrial tolerant Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic starch and cellulosic sugars universally to ethanol anaerobically [abstract]. Cambridge Healthtech Institute, Advanced Biofuels Development Summit.
Hughes, S.R. 2009. Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings [abstract]. LabAutomation 2009
Yoder, A., Pajak, L., Jackson Jr, J.S., Hughes, S.R. 2009. Creating libraries for commercial yeast strains through miniaturization of cloning and transformations using the BioRAPTR FRD Microfluidic workstation [abstract]. LabAutomation.
Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2008. Make use of lactic acid bacteria in biomass to biofuel [abstract]. International Symposium on Biocatalysis and Biotechnology.
Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D. 2008. Three-vector system for use on automated systems for high-level functional expression of value-added co-products with Xylose Isomerase and Xylulokinase in an industrial Saccharomyces cerevisiae strain [abstract].
Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D., Liu, S. 2008. Improving biocatalysts for the production of biofuels from lignocellulosic feedstocks [abstract]. U.S. Japan Natural Resources Meeting.
Rich, J.O., Bischoff, K.M., Hughes, S.R. 2008. Cellulosic ethanol production from Saccharomyces cerevisiae engineered for anaerobic conversion of pretreated lignocellulosic sugars to ethanol [abstract]. American Chemical Society. Paper ID1211547.
Hector, R.E., Qureshi, N., Hughes, S.R., Cotta, M.A. 2008. Expression of a heterologous xylose transporter in a Saccharomyces cerevisiae strain engineered to utilize xylose improves aerobic xylose consumption. Applied Microbiology and Biotechnology. 80(4):675-684.
Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2008. Proteomic approach for molecular mechanisms under ethanol stress in Lactobacillus buchneri [abstract]. American Society for Microbiology Meeting. 0-030. p. 155.
Hector, R.E., Qureshi, N., Hughes, S.R., Cotta, M.A. 2008. Expression of a heterologous xylose transporter in a Saccharomyces cerevisiae strain engineered to utilize xylose increases xylose uptake and improves xylose/glucose co-consumption [abstract]. In: Proceedings of the 30th Symposium on Biotechnology for Fuels and Chemicals, May 4-7, 2008, New Orleans, LA. p. 131.
Hughes, S.R., Rich, J.O. 2008. Ethanol production from modern biorefinery: Robotic platform for production of Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars to ethanol anaerobically [abstract]. Laboratory Robotics Information Group (LRIG). Seminar 1. p. 1.
Tasaki, K., Moser, B.R., Doll, K.M., Hughes, S.R. 2008. Biodiesel production using heterogenous catalyst [abstract]. International Biofuels Development Summit. p. 2.
Liu, S., Bischoff, K.M., Price, N.P., Hughes, S.R. 2007. Carbohydrate utilization and detection of a nucleotide hydrolase in Lactobacillus buchneri [abstract]. 3rd International Symposium on Biocatalysis and Biotechnology. p. 49.
Hughes, S.R., Chen, H., Tsai, D., Thompson, W., Dowd, P.F., Hooberman, B., Benjamin, L., Alexander, L.J., Rosentrater, K.A., Rich, J.O. 2007. Regulatory steps associated with use of value-added recombinant proteins and peptides screened in high-throughput for expression in genetically engineered starch and cellulosic fuel ethanol yeast strains [abstract]. FDA Meeting. Talk 1.
Hughes, S.R., Bang, S., Bleakley, B. 2007. Industrially useful microorganisms isolated from deep underground science and engineering laboratory (DUSEL) mine biofilms [abstract]. Meeting with South Dakota State Mine & Technology (SDSM&T) and South Dakota State University (SDSU). BioDUSEL Section, Day 2, Talk 3. p. 15.
Qureshi, N., Saha, B.C., Hector, R.E., Hughes, S.R., Cotta, M.A. 2008. Butanol production from wheat straw by simultaneous saccharification and fermentation using Clostridium beijerinckii: Part I - batch fermentation. Biomass and Bioenergy. 32:168-175.
Bischoff, K.M., Liu, S., Hughes, S.R. 2007. Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361. Process Biochemistry. 42:1150-1154.
Hughes, S.R., Dowd, P.F., Hector, R.E., Riedmuller, S.B., Bartolett, S., Mertens, J.A., Qureshi, N., Liu, S., Bischoff, K.M., Li, X., Jackson Jr, J.S., Sterner, D., Panavas, T., Cotta, M.A., Farrelly, P.J., Butt, T. 2007. High-throughput fully automated construction of a multiplex library of mutagenized open reading frames for an insecticidal peptide using a plasmid-based functional proteomic robotic workcell with improved vacuum system. Journal of Laboratory Automation. 12(4):202-212.
Qureshi, N., Saha, B.C., Li, X., Hughes, S.R., Cotta, M.A. 2007. Recent advances in bioconversion of agricultural biomass to butanol by fermentation: employing potential of available renewable resources to produce a superior biofuel [abstract]. American Chemical Society. Paper No. 178.
Hughes, S.R., Mertens, J.A., Qureshi, N., Cotta, M.A., Bischoff, K.M., Liu, S., Li, X. 2007. Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Improved Growth on Xylose Expressing Xylanase A(Xyn A) and Celulase F(Cel F) from Anaerobic Fungi Orpinomyces [abstract]. 2007 World Congress on Industrial Biotechnology and Bioprocessing. Seminar 2. p. 19.
Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Production of highly active lignocellulose-degrading enzymes of anaerobic fungi by industrially relevant fungi [abstract].
Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Expression of an AT-rich xylanase gene from the anaerobic fungus Orpinomyces sp. strain PC-2 in and secretion of the heterologous enzyme by Hypocrea jecorina. Applied Microbiology and Biotechnology. 74:1264-1275.
Bischoff, K.M., Rooney, A.P., Li, X., Liu, S., Hughes, S.R. 2006. Purification and characterization of a family 5 endoglucanase from a moderately thermophilic strain of Bacillus licheniformis. Biotechnology Letters. 28:1761-1765.
Qureshi, N., Saha, B.C., Hughes, S.R., Cotta, M.A. 2006. Production of acetone butanol (AB) from agricultural residues using Clostridium acetobutylicum in batch reactors coupled with product recovery [abstract]. Proceedings of the Ninth International Workshop on the Regulation of Metabolism, Genetics, and Development of the Solvent and Acid Forming Clostridia. p. 29.
Li, X., Jordan, D.B., Ximenes, E.A., Cotta, M.A., Dien, B.S., Hughes, S.R. 2006. Codon optimization of an A+T rich xylanase gene from the anaerobic fungus Orpinomyces PC-2 for expression by Trichoderma reesei [abstract]. In: Proceedings of the 9th International Workshop on Trichoderma and Gliocladium, April 6-8, 2006, Vienna, Austria. Paper No. T3.
Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Qureshi, N., Farrelly, P., Cotta, M.A. 2006. Automated strategy using a functional proteomic assay to identify and isolate cellulase F mutants with improved activity from multiplexed sets of plasmid [abstract]. PepTalk 2006. p. 10.
Hughes, S.R., Riedmuller, S., Li, X., Qureshi, N., Liu, S., Bischoff, K.M., Cotta, M.A., Farrelly, P. 2006. Mass transformation of plasmid libraries of cDNA or mutagenized clone sets into yeast or bacteria using a functional proteomic robotic workcell [abstract]. PepTalk 2006. p. 10.
Qureshi, N., Dien, B.S., Nichols, N.N., Liu, S., Hughes, S.R., Iten, L.B., Saha, B.C., Cotta, M.A. 2005. Continuous production of ethanol in high productivity bioreactors using genetically engineered Escherichia coli FBR5: membrane and fixed cell reactors [extended abstract]. American Institute of Chemical Engineers. Paper No. 589g.
Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Jordan, D.B., Li, X., Qureshi, N., Cotta, M.A., Farrelly, P.J., Bischoff, K.M. 2005. Functional proteomic workcell for high volume plasmid preparations for repeated in vitro protein expression and high throughput screening to identify mutant enyzmes for use at low pH [abstract]. Optimization High-throughput Cultures for Bioprocessing 2005. 13:3.
Bischoff, K.M., Skinner-Nemec, K., Leathers, T.D., Hughes, S.R. 2005. Antimicrobial susceptibility of bacterial contaminants from a wet-mill ethanol plant [abstract]. Society for Industrial Microbiology. Poster P42.
Bischoff, K.M., Li, X., Rooney, A.P., Liu, S., Hughes, S.R. 2005. Characterization of carboxymethylcellulase activity from Geobacillus stearothermophilus [abstract]. Society for Industrial Microbiology. Paper #P38.
Liu, S., Dien, B.S., Cotta, M.A., Bischoff, K.M., Hughes, S.R. 2005. Lactobacillus brevis: a potential biocatalyst for lignocellulosic biomass to ethanol [abstract]. Society of Industrial Microbiology. Paper #P05.
Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Bischoff, K.M., Cotta, M.A., Farrelly, P. 2005. Development of a liquid handler component for a plasmid-based functional proteomic robotic workcell. Journal of the Association for Laboratory Automation. 10(5):287-300.
Hughes, S.R., Riedmuller, S.B., Bischoff, K.M., Mertens, J.A., Li, X., Cotta, M.A., Farrelly, P.J. 2005. Development of a liquid handler component for a functional plasmid-based proteomic workcell that generates multiplex samples expresses in yeast [abstract]. Association for Laboratory Automation, LabAutomation 2005. Poster WP128.
Qureshi, N., Hughes, S.R., Maddox, I.S., Cotta, M.A. 2005. Energy efficient recovery of butanol from model solutions and fermentation broth by adsorption. Bioprocess and Biosystems Engineering. 27(4):215-222.
Hughes, S.R., Li, X., Cotta, M.A., Mertens, J.A., Bischoff, K.M., Riedmuller, S., Farrelly, P., Patel, M., Brown, L., Carter, L. 2005. A plasmid-based functional proteomic workcell used to optimize yeast for fuel ethanol production [abstract]. CHI's Annual PEP Talk Meeting, January 10-14, 2005, San Diego, California. p. 3.
Hughes, S.R., Li, X., Cotta, M.A., Mertens, J.A., Bischoff, K.M., Riedmuller, S., Farrelly, P., Patel, M., Brown, L., Carter, L. 2005. Modified liquid handler used to produce high throughput high quality plasmids for integration into plasmid-based functional proteomics workcell [abstract]. CHI's Annual PEP Talk Meeting, January 10-14, 2005, San Diego, California. Poster 151.
Hughes, S.R., Li, X., Cotta, M.A., Bischoff, K.M., Riedmuller, S., Farrelly, P. 2004. A plasmid-based functional proteomic workcell assay designed to screen Orpinomyces cellulase F NNY mutagenized libraries in high throughtput [abstract]. 36th Great Lakes Regional American Chemical Society Symposium, October 17-20, 2004, Peoria, Illinois. Poster 361.
Hughes, S.R., Li, X., Cotta, M.A., Bischoff, K.M., Riedmuller, S., Farrelly, P. 2004. A plasmid-based functional proteomic workcell used to optimize open reading frames and generate improved strains for commercial use [abstract]. 36th Great Lakes Regional American Chemical Society Symposium, October 17-20, 2004, Peoria, Illinois. Poster 361.