ARS | Publication request: Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars

Submitted to: Society of Industrial Microbiology Annual Meeting
Publication Type: Abstract
Publication Acceptance Date: August 14, 2008
Publication Date: August 14, 2008
Citation: Hughes, S.R., Javers, J., Labaer, J., Butt, T., Hector, R.E., Dien, B.S., Saha, B.C., Bischoff, K.M., Kohl, S., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars [abstract]. Society of Industrial Microbiology. Session 34, S164, p. 106.

Technical Abstract: Advanced automated high-throughput mass transformation of yeast full-genome libraries into Saccharomyces cerevisiae and screening for growth on xylose produced a yeast strain that is capable of fully utilizing pentose as well as hexose sugars anaerobically. This is the first yeast strain capable of strictly anaerobic growth on xylose and glucose simultaneously. Further study of this C5/C6 fermenting strain indicates optimum utilization of sugars from an acid hydrolysate of lignocellulosic feedstock. The paradigm for use of the new strain in industrial lignocellulosic ethanol production using acid pretreatment will be discussed.