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Copyright © 2008 Gutiérrez-Ortega et al; licensee BioMed Central Ltd. Respiratory syncytial virus glycoproteins uptake occurs through clathrin-mediated endocytosis in a human epithelial cell line Abel Gutiérrez-Ortega,1 Carla Sánchez-Hernández,2 and Beatriz Gómez-García  31Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas #800, Colinas de la Normal, C.P. 44270, Guadalajara, Jalisco, México 2Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Carretera Guadalajara-Nogales km 15.5, Las Agujas, C.P. 45110, Zapopan, Jalisco, México 3Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Circuito Interior, Ciudad Universitaria, DF México, México Corresponding author.Abel Gutiérrez-Ortega: agutierrster/at/gmail.com; Carla Sánchez-Hernández: sanchez.cv/at/gmail.com; Beatriz Gómez-García: begomez/at/servidor.unam.mx Received September 24, 2008; Accepted October 25, 2008. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | ||||
Abstract Cell-surface viral proteins most frequently enter the cell through clathrin or caveolae endocytosis. Respiratory syncytial virus antigen internalization by immune cells is via caveolin, however, uptake of paramyxovirus cell membrane proteins by non-immune cells is done through clathrin-coated pits. In this work, the uptake of respiratory syncytial virus cell surface glycoproteins by non-immune human epithelial cells was investigated through indirect immunofluorescence with polyclonal anti-RSV antibody and confocal lasser-scanner microscopy. Clathrin and caveolae internalization pathways were monitored through specific inhibitors monodansylcadaverine (MDC) and methyl-beta-cyclodextrin (MBCD), respectively. Internalization of RSV antigens was inhibited by MDC but not by MBCD, implying that clathrin-mediated endocytosis is the major uptake route of RSV antigens by an epithelial human cell line. | ||||
