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PubMed articles by:
Kosutic-Gulija, T.
Forcic, D.
Šantak, M.
Mazuran, R.
Virol J. 2008; 5: 79.
Published online 2008 July 10. doi: 10.1186/1743-422X-5-79.
PMCID: PMC2481254
Copyright © 2008 Kosutic-Gulija et al; licensee BioMed Central Ltd.
Genetic heterogeneity of L-Zagreb mumps virus vaccine strain
Tanja Kosutic-Gulija,corresponding author1 Dubravko Forcic,1 Maja Šantak,1 Ana Ramljak,2 Sanja Mateljak-Lukacevic,3 and Renata Mazuran1
1Department for Research and Development, Institute of Immunology Inc, Rockefeller Street 10, Zagreb, Croatia
2Department of Viral Vaccines, Institute of Immunology Inc, Rockefeller Street 10, Zagreb, Croatia
3Quality Control Department, Institute of Immunology Inc, Rockefeller Street 10, Zagreb, Croatia
corresponding authorCorresponding author.
Tanja Kosutic-Gulija: tgulija/at/imz.hr; Dubravko Forcic: dforcic/at/imz.hr; Maja Šantak: msantak/at/imz.hr; Ana Ramljak: Ana.Ramljak/at/pliva.hr; Sanja Mateljak-Lukacevic: smateljak/at/imz.hr; Renata Mazuran: rmazuran/at/imz.hr
Received April 24, 2008; Accepted July 10, 2008.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

 
Abstract

Background
The most often used mumps vaccine strains Jeryl Lynn (JL), RIT4385, Urabe-AM9, L-Zagreb and L-3 differ in immunogenicity and reactogenicity. Previous analyses showed that JL, Urabe-AM9 and L-3 are genetically heterogeneous.

Results
We identified the heterogeneity of L-Zagreb throughout the entire genome. Two major variants were defined: variant A being identical to the consensus sequence of viral seeds and vaccine(s) and variant B which differs from variant A in three nucleotide positions. The difference between viral variants in L-Zagreb strain is insufficient for distinct viral strains to be defined. We demonstrated that proportion of variants in L-Zagreb viral population depends on cell substrate used for viral replication in vitro and in vivo.

Conclusion
L-Zagreb strain should be considered as a single strain composed of at least two variant viral genomes.

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