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Copyright © 2008 Ludert et al; licensee BioMed Central Ltd. Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells Juan E Ludert,1 Clemente Mosso,1 Ivonne Ceballos-Olvera,1 and Rosa M del Angel  11Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del I.P.N., Mexico City, Mexico Corresponding author.Juan E Ludert: jludert/at/cinvestav.mx; Clemente Mosso: clemen_elche/at/hotmail.com; Ivonne Ceballos-Olvera: ivonneceol/at/yahoo.com.mx; Rosa M del Angel: rmangel/at/cinvestav.mx Received February 12, 2008; Accepted April 25, 2008. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | ||||
Abstract Current methods for dengue virus quantitation are either time consuming, technically demanding or costly. As an alternative, the commercial enzyme immunoassay Platelia™ Dengue NS1 AG (Bio-Rad Laboratories) was used to monitor semiquantitatively dengue virus replication in cultured cells. The presence of NS1 protein was evaluated in supernatants from Vero and C6/36 HT cells infected with dengue virus. The amount of NS1 detected in the supernatants of infected cells was proportional to the initial MOI used and to the time of post infection harvest. This immunoassay was also able to detect the presence of NS1 in the supernatants of infected human macrophages. Inhibition of dengue virus replication in C6/36 HT cells treated with lysosomotropic drugs was readily monitored with the use of this assay. These results suggest that the Platelia™ Dengue NS1 AG kit can be used as a fast and reliable surrogate method for the relative quantitation of dengue virus replication in cultured cells. | ||||
