Projects

Industrially Robust Enzymes and Microorganisms for Production of Sugars and Ethanol from Agricultural Biomass Appropriated (D)   Accession Number: 408893

Publications

Conversion of switchgrass to sugars and ethanol using dilute ammonium hydroxide pretreatment - (Abstract) - (06-May-09)

Evaluation of engineered xylose-fermenting industrial strains of Saccharomyces cerevisiae for improved ethanol production from lignocellulosic feedstocks - (Abstract) - (06-May-09)

Conversion of lower lignin mutants of Sorghum bicolor (L.) to ethanol - (Abstract) - (13-Mar-09)

Development of an Ethanol Yield Procedure for Dry Grind Corn Processing - (Peer Reviewed Journal) - (23-Feb-09)

Automated yeast transformation protocol to engineer S. cerevisiae strains for cellulosic ethanol production with open reading frames that express proteins binding to xylose isomerase identified using robotic two-hybrid screen - (Peer Reviewed Journal) - (19-Feb-09)

Automated yeast mating protocol using open reading frames from Saccharomyces cerevisiae genome to improve yeast strains for cellulosic ethanol production - (Peer Reviewed Journal) - (07-Jan-09)

Mass balances and analytical methods for biomass pretreatment experiments - (Book/Chapter) - (31-Oct-08)

Agricultural Residues and Energy Crops as Novel Substrates for Butanol Production by Fermentation - (Abstract)

Qureshi, N., Saha, B.C., Iten, L.B., Sarath, G., Dien, B.S., Cotta, M.A. 2008. Agricultural Residues and Energy Crops as Novel Substrates for Butanol Production by Fermentation [abstract]. In: Proceedings of 10th International Workshop and Conference on Regulation of Metabolism, Genetics, and Development of Solvent and Acid Forming Clostridia, September 28-October 1, 2008, Holland, The Netherlands. Paper No. 19.

Simultaneous Saccharification and Fermentation and Partial Saccharification and Co-fermentation of Lignocellulosic Biomass for Ethanol Production - (Book/Chapter) - (26-Sep-08)

Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system - (Peer Reviewed Journal)

Hughes, S.R., Sterner, D.E., Bischoff, K.M., Hector, R.E., Dowd, P.F., Qureshi, N., Bang, S.S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Dien, B.S., Mertens, J.A., Caughey, R.J., Liu, S., Butt, T.R., Labaer, J., Cotta, M.A., Rich, J.O. 2008. Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system. Plasmid Journal. 61:22-38.

Challenges of converting agricultural abundance to biofuels: Agricultural Research Service-United States Department of Agriculture Research - (Abstract) - (28-Aug-08)

Opportunities for increasing biomass conversion efficiencies to ethanol by engineering energy crops - (Abstract) - (17-Aug-08)

Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars - (Abstract)

Hughes, S.R., Javers, J., Labaer, J., Butt, T., Hector, R.E., Dien, B.S., Saha, B.C., Bischoff, K.M., Kohl, S., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars [abstract]. Society of Industrial Microbiology. Session 34, S164, p. 106.

Thin stillage fractionation using ultrafiltration: resistance in series model - (Peer Reviewed Journal)

Arora, A., Dien, B.S., Belyea, R.L., Wang, P., Singh, V., Tumbleson, M.E., Rausch, K.D. 2009. Thin stillage fractionation using ultrafiltration: resistance in series model. Bioprocess and Biosystems Engineering. 32(2):225-233.

Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars anaerobically to ethanol - (Abstract)

Hughes, S.R., Bischoff, K.M., Hector, R.E., Dien, B.S., Saha, B.C., Javers, J., Gibbons, W., Bang, S., Nelson, R., Standish, K., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars anaerobically to ethanol [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit, Seminar 6. p. 1.

Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain - (Abstract)

Hughes, S.R., Jackson Jr, J.S., Dowd, P.F., Hector, R.E., Bischoff, K.M., Sterner, D., Bang, S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Li, X., Caughey, R.J., Liu, S., Skory, C.D., Butt, T., Labaer, J., Dien, B.S., Saha, B.C., Bowen, M., Qureshi, N., Rich, J.O., Leathers, T.D., Cotta, M.A., Farrelly, P., Slininger P.J. 2008. Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit. Poster #1. p. 4.

Evaluating low lignin mutants of forage sorghum for increased conversion efficiency to sugars and ethanol - (Abstract)

Dien, B.S., Sarath, G., Pedersen, J.F., Funnell-Harris, D.L., Sattler, S.E., Nichols, N.N. 2008. Evaluating low lignin mutants of forage sorghum for increased conversion efficiency to sugars and ethanol. Biotechnology for Fuels and Chemicals. p. 267.

Energy crops for ethanol: a processing perspective - (Abstract)

Dien, B.S. 2008. Energy crops for ethanol: a processing perspective. International Crop Science. Paper No. CS4-S4-06.

Energy crops for ethanol: a processing perspective - (Proceedings/Symposium)

Dien, B.S., Sarath, G., Pedersen, J.F., Vogel, K.P., Jung, H.G., Sattler, S.E., Casler, M.D., Mitchell, R., Cotta, M.A. 2008. Energy crops for ethanol: a processing perspective. In: Proceedings of the 5th International Crops Science Congress, April 13-18, 2008, Jeju Island, Korea. p. 1-5.

Fungal metabolism of fermentation inhibitors present in corn stover dilute acid hydrolysate - (Peer Reviewed Journal)

Nichols, N.N., Sharma, L.N., Mowery, R.A., Chambliss, C.K., Van Walsum, G.P., Dien, B.S., Iten, L.B. 2008. Fungal metabolism of fermentation inhibitors present in corn stover dilute acid hydrolysate. Enzyme and Microbial Technology. 42(7):624-630.

Production of Ethanol from Corn and Sugarcane - (Book/Chapter)

Nichols, N.N., Dien, B.S., Monceaux, D.A., Bothast, R.J. 2008. Production of ethanol from corn and sugarcane. In: Wall, J., Harwood, C.S., Demain, A.L., editors. Bioenergy. Chapter 1. Washington, DC: ASM Press. p. 3-15.

Process review of lignocellulose biochemical conversion to fuel ethanol - (Proceedings/Symposium)

Dien, B.S. 2007. Process review of lignocellulose biochemical conversion to fuel ethanol. Proceedings of U.S. Frontiers of Engineering. p. 65-74.

Developing herbaceous energy crops as feedstocks for biofuel production - (Proceedings/Symposium)

Dien, B.S., Jung, H.G., Vogel, K.P., Casler, M.D., Lamb, J.F., Iten, L.B., Mitchell, R., Sarath, G. 2007. Developing herbaceous energy crops as feedstocks for biofuel production. In: Proceedings of the UJNR Food and Agricultural Panel, October 21-25, 2007, Tsukuba, Japan. p. 137-140.

Butanol Production by Clostridium Beijerinckii. Part I. Use of Acid and Enzyme Hydrolysed Corn Fiber - (Peer Reviewed Journal)

Qureshi, N., Ezeji, T.C., Ebener, J., Dien, B.S., Cotta, M.A., Blaschek, H.P. 2008. Butanol production by Clostridium beijerinckii. Part I. Use of acid and enzyme hydrolysed corn fiber. Bioresource Technology. 99:5915-5922.

Enzyme characterization for hydrolysis of AFEX and liquid hot-water pretreated distillers' grains and their conversion to ethanol - (Peer Reviewed Journal)

Dien, B.S., Ximenes, E.A., O'Bryan, P.J., Moniruzzaman, M., Li, X., Balan, V., Dale, B., Cotta, M.A. 2008. Enzyme characterization for hydrolysis of AFEX and liquid hot-water pretreated distillers' grains and their conversion to ethanol. Bioresource Technology. 99(12):5216-5225.

Composition of corn dry-grind ethanol by-products: DDGS, wet cake, and thin stillage - (Peer Reviewed Journal)

Kim, Y., Mosier, N.S., Hendrickson, R., Ezeji, T., Blaschek, H., Dien, B.S., Cotta, M.A., Dale, B., Ladisch, M.R. 2008. Composition of corn dry-grind ethanol by-products: DDGS, wet cake, and thin stillage. Bioresource Technology. 99(12):5165-5176.

Assessment of Bermudagrass and Bunch Grasses as Feedstock for Conversion to Ethanol - (Peer Reviewed Journal)

Anderson, W.F., Dien, B.S., Brandon, S.K., Peterson, J. 2008. Assessment of bermudagrass and bunch grasses as feedstock for conversion to ethanol. Applied Biochemistry and Biotechnology. 145(1-3):13-21.

Fiber Separated from Distillers Dried Grains with Solubles As a Feedstock for Ethanol Production to Increase Output from Dry Grind Corn Plants - (Peer Reviewed Journal)

Srinivasan, R., Dien, B.S., Rausch, K.D., Tumbleson, M.E., Singh, V. 2007. Fiber Separated from Distillers Dried Grains with Solubles as a Feedstock for Ethanol Production. Cereal Chemistry. 84(6):563-566.

Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis - (Peer Reviewed Journal)

Liu, S., Dien, B.S., Nichols, N.N., Bischoff, K.M., Hughes, S.R., Cotta, M.A. 2007. Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis. FEMS Microbiolology Letters. 274(2):291-297.

A Primer for Lignocellulose Biochemical Conversion to Fuel Ethanol - (Proceedings/Symposium)

Dien, B.S. 2007. A primer for lignocellulose biochemical conversion to fuel ethanol. Proceedings of the Fifth International Starch Technology Conference. p. 5.1-5.9.

Monensin inhibits growth of bacterial contaminants from fuel ethanol plants - (Abstract)

Bischoff, K.M., Liu, S., Dien, B.S. 2007. Monensin inhibits growth of bacterial contaminants from fuel ethanol plants [abstract]. American Society for Microbiology. Poster #0-010. p. 60.

Evaluating Herbaceous Perennials As Energy Crops for Production of Fuel Ethanol - (Abstract) - (02-May-07)

A Novel Pretreatment (Diethyl Oxalate) Releasing Fermentable Sugars and Useful Fibers from Lignocellulose - (Abstract) - (02-May-07)

Removal of Inhibitors from Biomass Sugars Using a Biological Process - (Abstract) - (02-May-07)

Assessment of Bermudagrass as a Feedstock for Ethanol - (Abstract)

Anderson, W.F., Dien, B.S., Peterson, J., Brandon, S.K., Akin, D.E. 2007. Assessment of bermudagrass as a feedstock for ethanol. [abstract]. ASA-CSSA-SSSA International Meetings, November 4-8, 2007, New Orleans, Louisiana. Cd Rom.

Developing Herbaceous Energy Crops As Feedstocks for Bioethanol Production - (Abstract)

Dien, B.S., Cotta, M.A., Jung, H.G., Vogel, K.P. 2007. Developing herbaceous energy crops as feedstocks for bioethanol production [abstract]. American Chemical Society. Paper No. Agro-148.

Biological Abatement for Removal of Inhibitors from Biomass Sugars - (Abstract) - (29-Mar-07)

Issues in the production and conversion of lignocellulosic biomass crops to ethanol - (Abstract)

Jung, H.G., Dien, B.S., Vogel, K.P. 2007. Issues in the production and conversion of lignocellulosic biomass crops to ethanol [abstract]. 233rd American Chemical Society National Meeting & Exposition, March 25-29, 2007, Chicago, IL. p. 107.

Production of Highly Active Lignocellulose-Degrading Enzymes of Anaerobic Fungi by Industrially Relevant Fungi - (Abstract)

Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Production of highly active lignocellulose-degrading enzymes of anaerobic fungi by industrially relevant fungi [abstract].

On-Farm Pretreatment Technologies for Improving Enzymatic Digestibility of Cellulose and Hemicellulose Present in Perennial Grass and Corn Stover - (Abstract)

Digman, M.F., Shinners, K.J., Dien, B.S., Muck, R.E., Weimer, P.J. 2007. On-farm pretreatment technologies for improving enzymatic digestibility of cellulose and hemicellulose present in perennial grass and corn stover [abstract]. Abstracts of the 29th Symposium on Biotechnology for Fuels and Chemicals, April 29-May 2, 2007, Denver, Colorado. p. 147.

On-Farm Pretreatment Technologies for Improving Enzymatic Digestibility of Cellulose and Hemicellulose Present in Perennial Grass - (Proceedings/Symposium)

Digman, M.F., Shinners, K.J., Dien, B.S., Hatfield, R.D., Li, X., Muck, R.E., Weimer, P.J. 2007. On-farm pretreatment technologies for improving enzymatic digestibility of cellulose and hemicellulose present in perennial grass. In: Proceedings of 2007 Annual Meeting of American Society of Agricultural and Biological Engineers, June 17, 2007, Minneapolis, Minnesota. Paper No. 071021.

A Primer for Lignocellulose Biochemical Conversion to Fuel Ethanol - (Book/Chapter) - (20-Jan-07)

Genotypic Evaluation of Bermudagrass for Conversion to Ethanol - (Abstract)

Anderson, W.F., Dien, B.S., Peterson, J., Akin, D.E. 2007. Genotypic Evaluation of Bermudagrass for Conversion to Ethanol. Proceedings: 29th Symposium on Biotechnology for Fuels and Chemicals, Denver, CO, April 29-May 2, 2007.

Issues in the production and conversion of lignocellulosic biomass crops to ethanol - (Proceedings/Symposium)

Jung, H.G., Dien, B.S., Vogel, K.P. 2007. Issues in the production and conversion of lignocellulosic biomass crops to ethanol. 233rd American Chemical Society National Meeting, March 25-29, 2007, Chicago, Illinois. Available: http://oasys2.confex.com/acs/233nm/techprogram/P1061547.htm.

Issues in the production and conversion of lignocellulosic biomass crops to ethanol - (Proceedings/Symposium)

Jung, H.G., Dien, B.S., Vogel, K.P. 2007. Issues in the production and conversion of lignocellulosic biomass crops to ethanol. In: 38th Northwest Alfalfa Seed Growers Association Winter Seed Conference Proceedings, January 22-23, 2007, Las Vegas, Nevada. p. 17-19.

Issues in the production and conversion of lignocellulosic biomass crops to ethanol - (Proceedings/Symposium)

Jung, H.G., Dien, B.S., Vogel, K.P. 2007. Issues in the production and conversion of lignocellulosic biomass crops to ethanol. In: Midwest Forage Association Symposium and Annual Meeting Proceedings, January 30-31, 2007, Wisconsin Dells, Wisconsin. CD-ROM.

Forage Energy Crops As Feedstocks for Production of Fuel Ethanol - (Abstract)

Cotta, M.A., Dien, B.S., Iten, L.B., Jung, H.G., Lamb, J.F., Vogel, K.P., Mitchell, R., Sarath, G., Casler, M.D., Weimer, P.J. 2006. Forage energy crops as feedstocks for production of fuel ethanol [abstract]. Biocatalysis and Bioenergy. p. 21.

Expression of An at-Rich Xylanase Gene from the Anaerobic Fungus Orpinomyces Sp. Strain Pc-2 in and Secretion of the Heterologous Enzyme by Hypocrea Jecorina - (Peer Reviewed Journal)

Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Expression of an AT-rich xylanase gene from the anaerobic fungus Orpinomyces sp. strain PC-2 in and secretion of the heterologous enzyme by Hypocrea jecorina. Applied Microbiology and Biotechnology. 74:1264-1275.

Enzyme Production by Industrially Relevant Fungi Cultured on Co-Product from Corn Dry Grind Ethanol Plants - (Peer Reviewed Journal)

Ximenes, E., Dien, B.S., Ladisch, M.R., Mosier, N., Cotta, M.A., Li, X. 2007. Enzyme production by industrially relevant fungi cultured on coproduct from corn dry grind ethanol plants. Applied Biochemistry and Biotechnology. 136-140:171-183.

Hemicellulases for Mediating Biomass Saccharification - (Proceedings/Symposium)

Jordan, D.B., Dien, B.S., Li, X., Cotta, M.A. 2006. Hemicellulases for mediating biomass saccharification. In: Proceedings of Renewable Energy 2006, October 9-13, 2006, Makuhari Messe, Chiba, Japan. p. 1036-1041.

Hemicellulases for Mediating Biomass Saccharification - (Abstract) - (13-Oct-06)

Review of Current Research Directions for Producing Biological Ethanol at the National Center for Agricultural Utilization Research - (Abstract)

Dien, B.S., Saha, B.C. 2006. Review of current research directions for producing biological ethanol at the National Center for Agricultural Utilization Research [abstract]. Advancing Renewable Energy. p. 27.

Culture Containing Biomass Acid Hydrolysate and Coniochaeta Ligniaria Fungus - (Patent Application)

Nichols, N.N., Lopez, M.J., Dien, B.S., Bothast, R.J. 2006. Culture containing biomass acid hydrolysate and Coniochaeta ligniaria fungus. U.S. Patent 7,067,303.

Lignocellulose-Degrading Enzymes Produced by the Ascomycete Coniochaeta Ligniaria and Related Species: Application for a Lignocellulosic Substrate Treatment - (Peer Reviewed Journal)

Lopez, M.J., Vargas-Garcia, M., Suarez-Estrella, F., Nichols, N.N., Dien, B.S., Moreno, J. 2007. Lignocellulose-degrading enzymes produced by the ascomycete Coniochaeta ligniaria and related species: application for a lignocellulosic substrate treatment. Enzyme and Microbial Technology. 40:794-800.

Pretreatment and Hydrolysis of Distiller's Grains to Fermentable Sugars: An Integrated Approach by the Midwest Consortium for Sustainable Biobased Products and Energy - (Abstract)

Cotta, M.A., Dien, B.S., Li, X., Ladisch, M., Mosier, N., Tyner, W., Woodson, R., Blaschek, H., Dale, B., Shanks, B., Verkade, J. 2006. Pretreatment and hydrolysis of distiller's grains to fermentable sugars: an integrated approach by the Midwest Consortium for sustainable biobased products and energy [abstract]. Corn Utilization. Paper No. 24.

Identification and Transcriptional Profiling of Furoic Acid Metabolism Genes in Pseudomonas Putida - (Abstract)

Nichols, N.N., Mertens, J.A., Dien, B.S. 2006. Identification and transcriptional profiling of furoic acid metabolism genes in Pseudomonas putida [abstract]. American Society for Microbiology. Paper No. Q-449.

An Overview of Recent Advancements in Lignocellulose to Ethanol Conversion Technology - (Abstract)

Dien, B.S., Nichols, N.N., Li, X., Cotta, M.A. 2006. An overview of recent advancements in lignocellulose to ethanol conversion technology [abstract]. International Conference on Bioenergy. p. 8.

Enzyme Production by Industrially Relevant Fungi Growing on Feed Co-Product from Dry Mill Ethanol Plants - (Abstract)

Ximenes, E.A., Dien, B.S., Ladisch, M.R., Mosier, N., Cotta, M.A., Li, X. 2006. Enzyme production by industrially relevant fungi growing on feed co-product from dry mill ethanol plants [abstract]. Biotechnology for Fuels and Chemicals. p. 107.

Research Challenges and Opportunities for Cellulose Conversion Technology in a Dry Mill Pathway - (Abstract)

Ladisch, M., Tyner, W., Mosier, N., Cotta, M.A., Dien, B.S., Blaschek, H., Dale, B., Shanks, B., Petersen, G. 2006. Research challenges and opportunities for cellulose conversion technology in a dry mill pathway [abstract]. Biotechnology for Fuels and Chemicals. p. 48.

On-Farm Pretreatment of Biomass: Impact of Genetics and Management - (Abstract) - (08-Apr-06)

Codon Optimization of An A+t Rich Xylanase Gene from the Anaerobic Fungus Orpinomyces Pc-2 for Expression by Trichoderma Reesei - (Abstract)

Li, X., Jordan, D.B., Ximenes, E.A., Cotta, M.A., Dien, B.S., Hughes, S.R. 2006. Codon optimization of an A+T rich xylanase gene from the anaerobic fungus Orpinomyces PC-2 for expression by Trichoderma reesei [abstract]. In: Proceedings of the 9th International Workshop on Trichoderma and Gliocladium, April 6-8, 2006, Vienna, Austria. Paper No. T3.

Nitrogen Source and Mineral Optimization Enhance D-Xylose Conversion to Ethanol by the Yeast Pichia Stipitis Nrrl Y-7124 - (Peer Reviewed Journal)

Slininger, P.J., Dien, B.S., Gorsich, S.W., Liu, Z. 2006. Nitrogen source and mineral optimization enhance D-xylose conversion to ethanol by the yeast Pichia stipitis NRRL Y-7124. Applied Microbiology and Biotechnology. 72(6):1285-1296.

Development of Forage Crops As Feedstocks for Production of Fuel Ethanol - (Abstract)

Cotta, M.A., Dien, B.S., Jung, H.G., Vogel, K.P., Casler, M.D., Lamb, J.F., Weimer, P.J., Iten, L.B., Mitchell, R., Sarath, G. 2006. Development of forage crops as feedstocks for production of fuel ethanol [abstract]. International Conference on Bioenergy. p. I-5.

Chemical Composition and Response to Dilute-Acid Pretreatment and Enzymatic Saccharification of Alfalfa, Reed Canarygrass, and Switchgrass - (Peer Reviewed Journal)

Dien, B.S., Jung, H.G., Vogel, K.P., Casler, M.D., Lamb, J.F., Iten, L.B., Mitchell, R., Sarath, G. 2006. Chemical composition and response to dilute-acid pretreatment and enzymatic saccharification of alfalfa, reed canarygrass, and switchgrass. Biomass and Bioenergy. 30:880-891.

Enzymatic Saccharification of Hot-Water Pretreated Corn Fiber for Production of Monosaccharides - (Peer Reviewed Journal)

Dien, B.S., Li, X., Iten, L.B., Jordan, D.B., Nichols, N.N., O Bryan, P.J., Cotta, M.A. 2006. Enzymatic saccharification of hot-water pretreated corn fiber for production of monosaccharides. Enzyme and Microbial Technology. 39:1137-1144.

Continuous Production of Ethanol in High Productivity Bioreactors Using Genetically Engineered Escherichia Coli Fbr5: Membrane and Fixed Cell Reactors - (Abstract)

Qureshi, N., Dien, B.S., Nichols, N.N., Liu, S., Hughes, S.R., Iten, L.B., Saha, B.C., Cotta, M.A. 2005. Continuous production of ethanol in high productivity bioreactors using genetically engineered Escherichia coli FBR5: membrane and fixed cell reactors [extended abstract]. American Institute of Chemical Engineers. Paper No. 589g.

Continuous Production of Ethanol in High Productivity Bioreactors Using Escherichia Coli Fbr5: Membrane and Fixed Cell Reactors - (Abstract)

Qureshi, N., Dien, B.S., Nichols, N.N., Liu, S., Iten, L.B., Saha, B.C., Cotta, M.A. 2005. Continuous production of ethanol in high productivity bioreactors using Escherichia coli FBR5: membrane and fixed cell reactors [abstract]. American Institute of Chemical Engineers. Paper No. 589g.

Lactobacillus Brevis: a Potential Biocatalyst for Lignocellulosic Biomass to Ethanol - (Abstract)

Liu, S., Dien, B.S., Cotta, M.A., Bischoff, K.M., Hughes, S.R. 2005. Lactobacillus brevis: a potential biocatalyst for lignocellulosic biomass to ethanol [abstract]. Society of Industrial Microbiology. Paper #P05.

Ethanol Fermentation of Sugars in Corn Stover Dilute Acid Hydrolysates - (Abstract)

Nichols, N.N., Dien, B.S., Cotta, M.A. 2005. Ethanol fermentation of sugars in corn stover dilute acid hydrolysates [abstract]. Society of Industrial Microbiology. p. 85.

Mineral and Nitrogen Source Optimization Enhance D-Xylose Conversion to Ethanol by the Yeast Pichia Stipitis - (Abstract)

Slininger, P.J., Dien, B.S., Gorsich, S.W., Liu, Z. 2005. Mineral and nitrogen source optimization enhance d-xylose conversion to ethanol by the yeast Pichia stipitis [abstract]. Society of Industrial Microbiology Annual Meeting. Paper No. P07.

Genetically Engineered Escherichia Coli for Ethanol Production from Xylose: Substrate and Product Inhibition and Kinetic Parameters - (Peer Reviewed Journal)

Qureshi, N., Dien, B.S., Nichols, N.N., Saha, B.C., Cotta, M.A. 2006. Genetically engineered Escherichia coli for ethanol production from xylose: substrate and product inhibition and kinetic parameters. Institution of Chemical Engineers Transactions. 84(2):114-122.

Tolerance to Furfural-Induced Stress Is Associated with Pentose Phosphate Pathway Genes Zwf1, Gnd1, Rpe1,and Tkl1 in Saccharomyces Cerevisiae - (Peer Reviewed Journal)

Gorsich, S.W., Dien, B.S., Nichols, N.N., Slininger, P.J., Liu, Z., Skory, C.D. 2005. Tolerance to furfural-induced stress is associated with pentose phosphate pathway genes ZWF1, GND1, RPEL, and TKL1 in Saccharomyces cerevisiae. Applied Microbiology and Biotechnology. 71(3):339-349.

Enzymatic Saccharification of Pretreated Corn Fiber for Production of Sugars - (Abstract)

Dien, B.S., Li, X., Jordan, D.B., Nichols, N.N., Iten, L.B., Cotta, M.A. 2005. Enzymatic saccharification of pretreated corn fiber for production of sugars [abstract]. International Starch Technology. p. 90.

Use of Field Pea Starch As a Feedstock for Ethanol Fermentation - (Abstract)

Nichols, N.N., Dien, B.S., Wu, V., Cotta, M.A. 2005. Use of field pea starch as a feedstock for ethanol fermentation [abstract]. International Starch Technology. p. 96.

The Corn Ethanol Industry - (Book/Chapter)

Nichols, N.N., Dien, B.S., Bothast, R.J., Cotta, M.A. 2006. The corn ethanol industry. In: Minteer, S., editor. Alcoholic Fuels. Boca Raton, FL: CRS Press. p. 59-78.

Metabolic Engineering of a Lactobacillus Plantarum Double Ldh Knockout Strain for Enhanced Ethanol Production - (Peer Reviewed Journal)

Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2006. Metabolic engineering of a Lactobacillus plantarum double LDH knockout strain for enhanced ethanol production. Journal of Industrial Microbiology and Biotechnology. 33(1):1-7.

Hydrolysis and Fermentation of Pericarp and Endosperm Fiber Recovered from Enzymatic Corn Dry Grind Process - (Peer Reviewed Journal)

Dien, B.S., Johnston, D., Hicks, K.B., Cotta, M.A., Singh, V. 2005. Hydrolysis and fermentation of pericarp and endosperm fibers recovered from enzymatic corn dry-grind process. Cereal Chemistry. 82(5):616-620.

Ethanol Fermentation of Starch from Field Peas - (Peer Reviewed Journal)

Nichols, N.N., Dien, B.S., Wu, Y., Cotta, M.A. 2005. Ethanol fermentation of starch from field peas. Cereal Chemistry. 82(5):554-558.

Enzymatic Saccharification of Pretreated Corn Fiber for Production of Sugars - (Abstract)

Dien, B.S., Li, X., Cotta, M.A. 2005. Enzymatic saccharification of pretreated corn fiber for production of sugars [abstract]. Biotechnology for Fuels and Chemicals. Paper No. B-36.

Ethanol Fermentation by Overexpressing a Gram-Positive Pdc Gene in Lactobacillus Plantarum Strain Tf103 - (Abstract)

Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2005. Ethanol fermentation by overexpressing a Gram-positive PDC gene in Lactobacillus plantarum strain TF103 [abstract]. Biotechnology for Fuels and Chemicals Symposium Proceedings, May 1-4, 2005, Denver, Colorado. p. 158.

Metabolic Engineering of Lactobacillus Brevis for Ethanol Production - (Abstract)

Liu, S., Dien, B.S. 2005. Metabolic engineering of Lactobacillus brevis for ethanol production [abstract]. The World Congress on Industrial Biotechnology and Bioprocessing. p. 23.

Functional Expression of Bacterial Zymobacter Palmae Pyruvate Decarboxylase Gene in Lactic Acid Bacteria - (Peer Reviewed Journal)

Liu, S., Dien, B.S., Cotta, M.A. 2005. Functional expression of bacterial Zymobacter palmae pyruvate decarboxylase gene in lactic acid bacteria. Current Microbiology. 50:1-6.

Review of Process for Producing Corn Fiber Oil and Ethanol from "quick Fiber" - (Review Article)

Dien, B.S., Nagle, N., Singh, V., Moreau, R.A., Tucker, M.P., Nichols, N.N., Johnston, D., Cotta, M.A., Hicks, K.B., Nguyen, Q., Bothast, R.J. 2005. Review of process for producing corn fiber oil and ethanol from "Quick Fiber." International Sugar Journal. 107(1275):187-191.

Profile of Enzyme Production of Trichoderma Reesei Grown on Corn Fiber Fractions - (Peer Reviewed Journal)

Li, X., Dien, B.S., Cotta, M.A., Wu, Y., Saha, B.C. 2005. Profile of enzyme production of Trichoderma reesei grown on corn fiber fractions. Applied Biochemistry and Biotechnology. 121-124:321-334.

Converting Herbaceous Energy Crops to Bioethanol: a Review with Emphasis on Pretreatment Processes - (Book/Chapter)

Dien, B.S., Iten, L.B., Skory, C.D. 2005. Converting herbaceous energy crops to bioethanol: a review with emphasis on pretreatment processes. In: Hou, C.T., editor. Handbook of Industrial Biocatalysis. Chapter 23. Boca Raton, FL: Taylor & Francis Group. p. 1-11.

Bioabatement to Remove Inhibitors from Biomass-Derived Sugar Hydrolysates - (Peer Reviewed Journal)

Nichols, N.N., Dien, B.S., Guisado, G.M., Lopez, M.J. 2005. Bioabatement to remove inhibitors from biomass-derived sugar hydrolysates. Applied Biochemistry and Biotechnology. 121-124:379-390.

Industrial Scale-Up of Ph Controlled Liquid Hot Water Pretreatment of Corn Fiber for Fuel Ethanol Production - (Peer Reviewed Journal)

Mosier, N.S., Hendrickson, R., Brewer, M., Ho, N., Sedlak, M., Dreshel, R., Welch, G., Dien, B.S., Aden, A., Ladisch, M.R. 2005. Industrial scale-up of ph-controlled liquid hot water pretreatment of corn fiber for fuel ethanol production. Applied Biochemistry and Biotechnology. 125:77-97.

In Vitro Gas Production As a Surrogate Measurement of the Fermentability of Cellulosic Biomass - (Peer Reviewed Journal)

Weimer, P.J., Dien, B.S., Springer, T.L., Vogel, K.P. 2005. In vitro gas production as a surrogate measurement of the fermentability of cellulosic biomass. Applied Microbiology Biotechnology. 67:52-58.

Recombinant Biocatalysts for Converting Sugar Mixtures to Lactic Acid - (Abstract)

Dien, B.S., Whitehead, T.R., Nichols, N.N., Skory, C.D., Cotta, M.A. 2004. Recombinant biocatalysts for converting sugar mixtures to lactic acid [abstract]. Great Lakes Regional American Chemical Society Symposium. Paper No. 40.

A Biological Approach to Removing Inhibitory Compounds from Biomass Sugars to Be Used for Fermentation. - (Abstract)

Nichols, N.N., Dien, B.S., Lopez, M.J. 2004. A biological approach to removing inhibitory compounds from biomass sugars to be used for fermentation [abstract]. Great Lakes Regional American Chemical Society Symposium. Paper No. 36.

Advances on Enzymatic Saccharification of Lignocellulosic Biomass - (Abstract)

Li, X., Jordan, D.B., Dien, B.S., Cotta, M.A., Kane, P.M. 2004. Advances on enzymatic saccharification of lignocellulosic biomass [abstract]. Great Lakes Regional American Chemical Society. Paper No. 04-035.

Adsorbed Cell Dynamic Biofilm Reactor for Ethanol Production from Xylose and Corn Fiber Hydrolysate - (Abstract)

Qureshi, N., Brining, H.R., Iten, L.B., Dien, B.S., Nichols, N.N., Saha, B.C., Cotta, M.A. 2004. Adsorbed cell dynamic biofilm reactor for ethanol production from xylose and corn fiber hydrolysate [abstract]. Great Lakes Regional American Chemical Society Symposium. p. 179.

The Saccharomyces Cerevisiae Pentose Phosphate Pathway Gene, Rpe1, functions in Furfural Tolerance During Fermentation - (Proceedings/Symposium)

Gorsich, S.W., Dien, B.S., Nichols, N.N., Slininger, P.J., Liu, Z. 2004. The Saccharomyces cerevisiae pentose phosphate pathway gene, rpe1, functions in furfural tolerance during fermentation [abstract]. Proceedings of the 11th International Congress on Yeasts in Science and Biotechnology. Paper No. PM24.

A Comparison Between Conversion of Pericarp and Endosperm Fiber from Corn into Ethanol - (Proceedings/Symposium)

Singh, V., Dien, B.S., Johnston, D., Hicks, K.B., Cotta, M.A. 2004. A comparison between conversion of pericarp and endosperm fiber from corn into ethanol. Proceedings of ASAE Annual International Meeting. p. 1-15.

Identification of Saccharomyces Cerevisiae Genes Involved in Furfural Tolerance During Fermentation. - (Abstract)

Gorsich, S.W., Slininger, P.J., Liu, Z., Nichols, N.N., Dien, B.S. 2004. Identification of Saccharomyces cerevisiae genes involved in furfural tolerance during fermentation [abstract]. Proceedings of the Yeast Genetics and Molecular Biology. Abstract No. 166A, p. 104.

A Standardized Laboratory Procedure for Ethanol Yield Measurement - (Abstract)

Tumbleson, M., Lemuz, C., Dien, B.S., Singh, V., Belyea, R., Rausch, K. 2004. A standardized laboratory procedure for ethanol yield measurement [abstract]. International Fuel Ethanol Workshop. Paper No. 61.

Ethanol Yield Determination for Dry Grind Corn Processing - (Proceedings/Symposium)

Lemuz, C.R., Dien, B.S., Tumbleson, M.E., Singh, V., Rausch, K.D. 2004. Ethanol yield determination for dry grind corn processing. Proceedings Corn Utilization and Technology Conference. p. 61.

Expression Analysis of Pyruvate Decarboxylase Genes from Zymobacter Palmae and Sarcina Ventriculi in Gram-Positive Lactic Acid Bacteria - (Abstract)

Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2004. Expression analysis of pyruvate decarboxylase genes from Zymobacter palmae and Sarcina ventriculi in Gram-positive lactic acid bacteria [abstract]. American Society for Microbiology. Paper No. O-099.

Engineering Klebsiella Oxytoca for Production of Lactic Acid from Hexose and Pentose - (Abstract)

Dien, B.S., Whitehead, T.R., Nichols, N.N., Cotta, M.A. 2004. Engineering klebsiella oxytoca for production of lactic acid from hexose and pentose [abstract]. Biotechnology for Fuels and Chemicals. Paper No. 2-08.

Growth of and Enzyme Production by Trichoderma Reesei on Corn Fiber Fractions - (Abstract)

Li, X., Dien, B.S., Cotta, M.A. 2004. Growth of and enzyme production by Trichoderma reesei on corn fiber fractions [abstract]. Biotechnology For Fuels And Chemicals. p. 25.

Genetically Engineered Escherichia Coli for Ethanol Production from Pentose Sugars: Substrate and Product Inhibition and Kinetic Parameters - (Abstract)

Qureshi, N., Dien, B.S., Nichols, N.N., Cotta, M.A. 2004. Genetically engineered Escherichia coli for ethanol production from pentose sugars: substrate and product inhibition and kinetic parameters [abstract]. Biotechnology for Fuels and Chemicals. Paper No. 5-23.

Use of the Ascomycete Coniochaeta Ligniaria to Remove Inhibitors from Biomass-Derived Sugars - (Abstract)

Nichols, N.N., Dien, B.S., Guisado, G.M., Lopez, M.J. 2004. Use of the ascomycete coniochaeta ligniaria to remove inhibitors from biomass-derived sugars [abstract]. Biotechnology For Fuels And Chemicals Symposium. Paper No. 2-10.

Adaptive Response of Yeasts to Furfural and 5-Hydroxymethylfurfural and New Chemical Evidence for Hmf Conversion to 2,5-Bis-Hydroxymethylfuran - (Peer Reviewed Journal)

Liu, Z., Slininger, P.J., Dien, B.S., Berhow, M.A., Kurtzman, C.P., Gorsich, S.W. 2004. Adaptive response of yeasts to furfural and 5-hydroxymethylfurfural and new chemical evidence for hmf conversion to 2,5-bis-hydroxymethylfuran. Journal of Industrial Microbiology and Biotechnology. 31:345-352. Available http://dx.doi.org/10.1007/s10295-004-0148-3.

Fermentation of Hexose and Pentose Sugar Mixtures to Lactic Acid by Recombinant Bacteria - (Abstract)

Dien, B.S., Nichols, N.N., Cotta, M.A. 2003. Fermentation of hexose and pentose sugar mixtures to lactic acid by recombinant bacteria [abstract]. American Institute Of Chemical Engineers. Paper No. 161D.

Optimization of Controlled Ph Liquid Hot Water Pretreatment of Corn Fiber and Stover - (Abstract)

Mosier, N.S., Kim, Y., Zeng, M., Hendrickson, R., Dien, B.S., Welsh, G., Ladisch, M. 2003. Optimization of controlled pH liquid hot water pretreatment of corn fiber and stover [abstract]. American Institute Of Chemical Engineers. Paper No. 163d.

Development of Microbial Biocatalysts to Produce Fuels from Agricultural Biomass - (Proceedings/Symposium)

Cotta, M.A., Nichols, N.N., Dien, B.S., Li, X. 2003. Development of microbial biocatalysts to produce fuels from agricultural biomass. Proceedings 32nd annual United States Japan Natural Resources Protein Panel. p. 387-393.

Properties of a Recombinant Beta-Glucosidase from the Polycentric Anaerobic Fungus Orpinomyces Pc-2 and Its Application for Cellulose Hydrolysis - (Peer Reviewed Journal)

Li, X., Ljungdahl, L.G., Ximenes, E.A., Chen, H., Felix, C.R., Cotta, M.A., Dien, B.S. 2004. Properties of a recombinant beta-glucosidase from the polycentric anaerobic fungus Orpinomyces PC-2 and its application for cellulose hydrolysis. Journal of Applied Biochemistry and Biotechnology. 113:233-250.

Fermentation of "quick Fiber" Produced from a Modified Corn Milling Process into Ethanol and Recovery of Corn Fiber Oil - (Peer Reviewed Journal)

Dien, B.S., Nagle, N., Singh, V., Moreau, R.A., Nichols, N.N., Johnston, D., Cotta, M.A., Hicks, K.B., Nguyen, Q., Bothast, R.J. 2004. Fermentation of "quick fiber" produced from a modified corn milling process into ethanol and recovery of corn fiber oil. Journal of Applied Biochemistry and Biotechnology. 115:937-950.

Separation of Butanol from Fermentation Broth by Pervaporation: Mass Flux and Energy Balance - (Abstract)

Qureshi, N., Dien, B.S., Saha, B.C., Ezeji, T.C., Blaschek, H.P., Cotta, M.A. 2003. Separation of butanol from fermentation broth by pervaporation: mass flux and energy balance [abstract]. American Institute of Chemical Engineers. Paper No. 162B.

Conversion of Lignocellulose to Ethanol Using a Recombinant E. Coli Strain - (Abstract)

Dien, B.S., Iten, L.B., Nichols, N.N., Cotta, M.A. 2003. Conversion of lignocellulose to ethanol using a recombinant E. coli strain [abstract]. Society of Industrial Microbiology. p. 102.

Comparative Tolerance Studies Reveal Dose-Dependent Inhibition of Ethanologenic Yeasts by Furfural and 5-Hydroxymethylfurfural - (Abstract)

Liu, Z., Slininger, P.J., Dien, B.S., Kurtzman, C.P., Gorsich, S.W. 2004. Comparative tolerance studies reveal dose-dependent inhibition of ethanologenic yeasts by furfural and 5-hydroxymethylfurfural [abstract]. Society of Industrial Microbiology. Paper No. 40.

Bacteria Engineered for Fuel Ethanol Production: Current Status - (Review Article)

Dien, B.S., Cotta, M.A., Jeffries, T.W. 2003. Bacteria engineered for fuel ethanol production: current status. Applied Microbiology and Biotechnology. 63:258-266.

Isolation of Microorganisms for Biological Detoxification of Lignocellulosic Hydrolysates - (Peer Reviewed Journal)

Lopez, M.J., Nichols, N.N., Dien, B.S., Moreno, J., Bothast, R.J. 2004. Isolation of microorganisms for biological detoxification of lignocellulosic hydrolysates. Applied Microbiology And Biotechnology. 64:125-131.

Increasing the Ethanol Yield from a Bushel of Corn; Prospects for Biomass Conversion - (Abstract)

Dien, B.S., Bothast, R.J., Nichols, N.N., Cotta, M.A. 2003. Increasing the ethanol yield from a bushel of corn; prospects for biomass conversion [abstract]. International Starch Technology. Paper No. 2.

Conversion of Corn Fibrous Material into Ethanol - (Abstract)

DIEN, B.S., NICHOLS, N.N., LI, X., COTTA, M.A., BOTHAST, R.J. CONVERSION OF CORN FIBROUS MATERIAL INTO ETHANOL. 25TH SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS. 2003. ABSTRACT P. 68.

Potential to Improve Dry Mill Economics by Increasing Ethanol Yield from Corn Fiber Residue - (Abstract)

NAGLE, N., TUCKER, M.P., DIEN, B.S., COTTA, M.A., HICKS, K.B., MOREAU, R.A., SINGH, V., NGUYEN, Q. POTENTIAL TO IMPROVE DRY MILL ECONOMICS BY INCREASING ETHANOL YIELD FROM CORN FIBER RESIDUE. 25TH SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS SYMPOSIUM. 2003. ABSTRACT P. 20.

Corn Fiber Hydrolysis and Fermentation to Butanol Using Clostridium Beijerinckii Ba101 - (Abstract)

EBENER, J.M., QURESHI, N., EZEJI, T.C., BLASCHEK, H.P., DIEN, B.S., COTTA, M.A. CORN FIBER HYDROLYSIS AND FERMENTATION TO BUTANOL USING CLOSTRIDIUM BEIJERINCKII BA101. 25TH SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS. 2003. PAPER NO. 2-14.

Principles and Economics of Pretreating Cellulose in Water for Ethanol Production - (Abstract)

Mosier, N., Hendrickson, R., Dreschel, R., Dien, B.S., Bothast, R.J., Welch, G., Ladisch, M.R. 2003. Principles and economics of pretreating cellulose in water for ethanol production [abstract]. American Chemical Society. Paper No. 103.

Enhancement of Ethanol Yield from the Corn Dry Grind Process by Fermentation of the Kernel Fiber Fraction - (Peer Reviewed Journal)

Dien, B.S., Nichols, N.N., Iten, L.B., Bothast, R.J. 2004. Enhancement of ethanol yield from the corn dry grind process by fermentation of the kernel fiber fraction. In: Nelson, W.M., editor. Agricultural Applications in Green Chemistry. Chap. 6. Washington, DC:American Chemical Society. p. 63-77.

Pre-Treatment of Wet-Milled Corn Fiber to Improve Recovery of Corn Fiber Oil, and Phytosterols - (Peer Reviewed Journal)

Cereal Chem. 80:118-122, 2003

Removal of Furfural Formed During Biomass Pretreatment by Polymeric Adsorbents - (Abstract) - (01-May-02)

The U.S. Corn Ethanol Industry: An Overview of Current Technology and Future Prospects - (Review Article)

DIEN, B.S., BOTHAST, R.J., NICHOLS, N.N., COTTA, M.A. THE U.S. CORN ETHANOL INDUSTRY: AN OVERVIEW OF CURRENT TECHNOLOGY AND FUTURE PROSPECTS. INTERNATIONAL SUGAR JOURNAL. 2002. V. 104. P. 204-211.