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![](https://webarchive.library.unt.edu/eot2008/20090509221444im_/http://www.ars.usda.gov/incme/images/Research_head.gif) |
Research Project:
Positional Candidate Genes for Resistance to Marek's Disease by Screening for Marek's Disease Virus Meq-Regulated Genes
Location: Avian Disease and Oncology Laboratory
Project Number: 3635-31320-008-24
Project Type:
Reimbursable
Start Date: Jan 01, 2009
End Date: Dec 31, 2011
Objective:
1. Identify MDV Meq and c-Jun binding sites in the chicken genome.
2. Identify differentially expressed genes following MDV-reactivation in MSB-1 cells.
3. Determine if differentially expressed genes identified in objective 2 also show differentially expression between MD tumors and CD4+ T cells.
4. Identify differentially expressed genes as a function of genetic resistance status or the presence of Meq.
Approach:
1. Utilize chromatin immunoprecipitation (ChIP) using Meq or c-Jun antibodies and determine the DNA sequences via Solexa paired-end reads.
2. Isolate RNA from MSB-1 cells at time 0 and following MDV reactivation, and run on Affymetrix Chicken microarrays.
3. Compare by qRT-PCR the relative expression of key genes between MD tumors and CD4+ T cells.
4. Isolate RNA from line 6 (MD resistant) and line 7 (MD susceptible) CEF at various time before and after infection with either MDV or MDV lacking Meq, and run on Affymetrix Chicken microarrays.
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Last Modified: 05/08/2009
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