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Research Project: Iron Deficiency Chlorosis: Getting to the Root of the Problem

Location: Corn Insects and Crop Genetics Research

Project Number: 3625-21220-004-02
Project Type: Reimbursable

Start Date: Jun 25, 2007
End Date: Jul 31, 2009

Objective:
1. Molecular Marker Discovery: Develop molecular markers that have diagnostic potential in identifying IDC efficient genotypes, extend the usefulness of markers to a broader range of soybean germplasm, and make the markers available to soybean breeders. 2. Candidate Gene Discovery: Use state of the art genomic technologies and the forthcoming whole genome sequence of soybean to identify genes potentially involved in IDC efficiency or inefficiency. Convert these candidate genes into markers that can be used by soybean breeders.

Approach:
Near-isogenic lines representing iron efficient and inefficient genotypes will be grown under iron stress conditions in a hydroponic solution. Affymetrix GeneChips will be used by the Ames and the St. Paul groups to identify genes differentially expressed between the lines and thus, candidates for involvement in the iron stress response. SNPs will be identified in candidate genes and developed as molecular markers. These markers will be tested in a broad range of germplasm to look for association with iron efficiency. Viable markers will be provided to the public and relevant information will be placed into SoyBase, The Soybean Breeder's Toolbox.

   

 
Project Team
Shoemaker, Randy
 
Project Annual Reports
  FY 2008
 
Related National Programs
  Plant Genetic Resources, Genomics and Genetic Improvement (301)
  Plant Biological and Molecular Processes (302)
 
 
Last Modified: 01/14/2009
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