Tissue Preparation Overview
Antibody Purified
A specific population of cells is purified using an antibody, normally attached to a magnetic
bead
Bulk Tissue
Whole tissue specimens (>0.5 g) which contain a variety of malignant
and/or normal cell types. Tumor samples are selected to be enriched in
tumor cells.
Cell Lines
Cell lines are cultured cells grown in vitro.
Flow Sorted
Flow sorted cells are cells separated by flow cytometry.
Micro-Bulk Tissue
Whole tissue specimens (<0.5 g) which are of biological interest, but cannot
be utilized for traditional libraries due to their small size. Libraries are
prepared by a PCR-based method employing 10 cycles of PCR. See
Krizman Protocol 1.
Microdissected Tissue
An important goal of CGAP is to develop and evaluate methods to recover
and analyze mRNA from microdissected tissue samples. This is
accomplished by comparing and contrasting cDNA libraries using several
different methodologies. The three methods that have been used to date
are Krizman Protocol 1,
Krizman Protocol 2, and
Krizman Protocol R/D.
For additional details and analysis of microdissected tissues, see
the Molecular Profiling Initiative.
Short-term Culture
Short-term cultured cells are cells grown in vitro, but a
permenant cell line is not established. Thus, the cells might be
more like the original than an established culture.
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