Swack N, Ramirez M, Berberich S, Stapleton J; American Society for Microbiology. General Meeting.
Abstr Gen Meet Am Soc Microbiol. 1994; 94: 624 (abstract no. V-37).
Hygienic Laboratory, Univ. of Iowa, Iowa City.
Assays for the detection of HIV-1 antigen have been used for the detection of circulating virus early in infection and also for assessment of disease progression and response to antiviral therapy. However HIV-1 p24 antigen may be complexed with HIV antibody and thus escape detection by antigen capture immunoassay. Previous studies to assay virus after acid dissociation of the immune complexes was found to be time consuming and required large quantities of serum. These problems were significantly reduced by the use of alkaline dissociation which was significantly less labor intensive and required smaller amounts of serum. Ninety seven serum specimens obtained from patients at the University of Iowa AIDS Clinic were studied. All sera were found to be HIV-1 antibody positive by Western Blot analysis. The semiquantitative detection of p24 antigen was studied by antigen capture assays obtained from commercial sources (Abbott Laboratories and Organon Teknika). Antigen dissociation was performed with the Vironostika HIV-1 Antigen Base Dissociation Assay. Both antigen assays without dissociation found 35% of the sera to be positive for the presence of p24 antigen. Following treatment with the alkaline dissociation reagents 67% of the same sera were positive for the presence of p24 antigen. All of these could be neutralized by specific antibody to HIV. Quantitative p24 determinations performed by both the undissociation and alkaline dissociation procedures were compared with patient lymphocyte immunophenotyping studies.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Antigen-Antibody Complex
- Antigens, Viral
- Evaluation Studies
- HIV Antibodies
- HIV Antigens
- HIV Core Protein p24
- HIV Envelope Protein gp120
- HIV Envelope Protein gp41
- HIV Infections
- HIV Seropositivity
- Humans
- Iowa
- immunology
Other ID:
UI: 102207890
From Meeting Abstracts