Fleury HJ, Merel P, Pellegrin I, Recordon-Pinson P, Hung PV, Uyen NT, Lan NT; International Conference on AIDS (15th : 2004 : Bangkok, Thailand).
Int Conf AIDS. 2004 Jul 11-16; 15: abstract no. MoPeB3152.
Virology department, University of Bordeaux 2, Bordeaux, France
Background: Recent studies have shown that CRF01-AE (AE) is highly predominant in Ho Chi Minh City (HCMC), Vietnam. We have initiated the quantification of AE viral load with a real time PCR (rt-PCR) which had been previously designed on B subtype isolates by amplifying the LTR region. It was rapidly obvious that, as compared to bDNA hybrization (Bayer), the viral loads of AE samples were underestimated. Consequently, our purpose was to improve the quantification of viral loads of AE isolates. Methods: We have sequenced the LTR of Vietnamese AE isolates and, due to mismatches in the region of subtype B probe hybridization, designed a new probe (MGB-AE). We therefore determined the viral load of 32 AE plasma samples with rt-PCR using MGB-AE probe with a AE strain calibration, B probe with a B strain calibration, Roche HIV-1 Monitor and b DNA hybridization (Bayer). For rt-PCR, both lightcycler and prism (ABI) technologies were used. Results: Viral load quantitative results obtained with MGB-AE probe and AE calibration curve are well correlated with those obtained with bDNA and Monitor assays and clearly higher than those with rt-PCR using B probe and B strain calibration. The results are similar using both lightcycler and prism technologies. Conclusions: rt-PCR using prism with amplification in the LTR, MGB-AE probe and AE strain calibration is now routinely used in HCMC for viral load quantitation.
Publication Types:
Keywords:
- Base Sequence
- HIV-1
- Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Terminal Repeat Sequences
- Vietnam
- Viral Load
- genetics
- reverse transcriptase, Human immunodeficiency virus 1
Other ID:
UI: 102279456
From Meeting Abstracts