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Quantifying recent thymic emigrants (RTE) in blood and tonsils of HIV-1-infected patients before and after HAART with a real-time PCR assay for T-cell receptor excisional circles (TREC).

Zhang L, Ramratnam B, Lewin S, Skulsky E, Kim A, Tuttleton S, Markowitz M, Ho DD; Conference on Retroviruses and Opportunistic Infections.

7th Conf Retrovir Oppor Infect Jan 30 Feb 2 2000 Conf Retrovir Oppor Infect 7th 2000 San Franc Calif. 2000 Jan 30-Feb 2; 7: 98 (abstract no. 124).

Aaron Diamond AIDS Res. Ctr., New York.

Quantifying one particular species of TREC (termed alpha1 circle) that is formed in nearly 70% of all alphabeta T cells provides a way of assessing the number of RTE in blood. We have extended our studies by comparing the number of alpha1 circles in blood versus tonsillar tissue. First, in a cross-sectional study of 30 patients (age 33 +/- 6) who were treated with HAART early in the course of HIV-1 infection, the number of alpha1 circles in tonsillar tissue (2187 +/- 1642/10(6) cells) was not significantly different from that in PBMC (2954 +/- 2115/10(6) cells). The latter value is almost entirely overlapping with those found in age-matched normal controls. Second, in a longitudinal study of 13 patients (age 31 +/- 6) whose tonsils were biopsied before and after (within 1 year) HAART, the number of alpha1 circles in PBMC and tonsils remained stable. Specifically, the values in tonsillar biopsies before and after HAART were 1886 +/- 1012/10(6) cells and 1331 +/- 751/10(6) cells, respectively. The relative stability of alpha1 circle numbers in both PBMC and tonsil over the course of treatment is probably reflective of the relatively intact thymic function in the early stage of HIV-1 infection that we have observed previously. We conclude from this study that the number of RTE in blood is reflective of that in one particular lymphoid tissue compartment.

Publication Types:
  • Meeting Abstracts
Keywords:
  • Antiretroviral Therapy, Highly Active
  • Biological Assay
  • Case-Control Studies
  • Cross-Sectional Studies
  • DNA Primers
  • HIV Infections
  • HIV-1
  • Humans
  • Longitudinal Studies
  • Lymphoid Tissue
  • Polymerase Chain Reaction
  • Receptors, Antigen, T-Cell
  • T-Lymphocytes
  • Thymus Gland
  • Tonsil
  • analysis
  • blood
Other ID:
  • GWAIDS0005505
UI: 102243002

From Meeting Abstracts




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