Lefrere F, Mariotti M, Thauvin M, Lefrere JJ; International Conference on AIDS.
Int Conf AIDS. 1993 Jun 6-11; 9: 283 (abstract no. PO-B01-0887).
INTS, Hopital St-Antoine, Paris, France.
OBJECTIVES: To determine through quantitative PCR the evolution over time of the cellular viral DNA load in the 6 first years following HIV infection. METHODS: Six HIV-1 seropositive individuals with a known date of infection (all had a negative serology in the 6 months preceding the first HIV serology found positive) were prospectively followed-up with annual controls including CDC stage, CD4+ cell count and determination (performed in 1992 on frozen samples) of proviral copy (PVC) number per 150,000 PBMCs through quantitative DNA-PCR. At the end of the follow-up, all but one had CDC stage II/III. RESULTS: Assuming that the HIV-infected CD4+ cells contained predominantly 1 VC and that 1 microgram of cellular DNA corresponds to 150,000 PBMCs, and considering the proportion of CD4+ cells in the PBMCs, the ratio infected/uninfected CD4+ cells was as follows: TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSIONS: These results indicate that the proportion of HIV-infected CD4+ cells regularly increases after the two first years of infection.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- CD4 Lymphocyte Count
- CD4-Positive T-Lymphocytes
- DNA, Viral
- HIV Infections
- HIV Seropositivity
- HIV-1
- Polymerase Chain Reaction
- Prospective Studies
- Viral Load
Other ID:
UI: 102203761
From Meeting Abstracts