Hardy CT, Damrow TA, Kenny GE; American Society for Microbiology. Meeting.
Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17; 90: 342 (abstract no. T-36).
University of Washington, Washington State Public Health Laboratories, Seattle, WA
Scanning densitometry was studied as a method for quantitating specific HIV-1 antibodies on Western Blots. Serum samples displaying varying reactivity to HIV-1 antigens were diluted in two fold steps ranging (1:80-1:2,600,000) and tested with commercially available Western Blotting strips. The staining intensity as measured by scanning densitometry showed a plateau at high serum concentrations. Thereafter, staining intensities showed a shallow slope which decreased gradually over a one hundred to one thousand fold dilution range. For example, with one p24 serum which had a staining intensity of 34,000 at 1:80 dilution needed to be diluted 1:330,000 in order to reduce the staining intensity by 50%. Thus, the detection of changes in antibody levels to HIV-1 antigens by Western blotting requires the testing of broadly-spaced serum dilutions.
Publication Types:
Keywords:
- Blotting, Western
- Densitometry
- Glycoproteins
- HIV Antibodies
- HIV-1
- Immunologic Techniques
Other ID:
UI: 102197672
From Meeting Abstracts