Carmichael A, Sissons JP, Borysiewicz LK; International Conference on AIDS.
Int Conf AIDS. 1991 Jun 16-21; 7: 145 (abstract no. W.A.1214).
Department of Medicine, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, UK
OBJECTIVE: Major Histo-Compatibility (MHC) class I-restricted CD8+ cytotoxic T lymphocytes (CTLs) are part of the cellular immune response to human persistent virus infections. The aim of the study was to determine prospectively the relative frequency and specificity of CD8+ CTLs against HIV-1, and in particular whether there is variation in the specificity of these CTLs as the infection progresses. METHODS: CTL responses were studied in both bulk culture and limiting dilution analysis, which provides quantitative estimates of the frequency of antigen-specific CTL precursors. The large limiting dilution assays were automated using a computer-controlled robotic processor. Thirty subjects at different clinical stages of HIV-1 infection were recruited and MHC typed; peripheral blood mononuclear cells (PBMC) were stimulated in vitro with autologous irradiated HIV-1IIIB infected lymphoblasts and analysed in 51Cr release cytotoxicity assays against target cells consisting of MHC matched and mismatched lymphoblastoid B cells which had been infected with vaccinia recombinants expressing individual HIV-1IIIB genes. RESULTS: MHC restricted gag-specific and pol-specific CTLs were demonstrated in the same individual and in asymptomatic infected subjects the frequency of CTL precursors measured for each of these specificities was 1/20000 - 1/30000 PBMC. CONCLUSIONS: The HIV-1 specific CTL precursor frequency was high in the subjects studied, and we are correlating the CTL response with disease progression in both a cross-sectional and prospective manner.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Antigens, CD8
- CD8-Positive T-Lymphocytes
- Case-Control Studies
- Genes, gag
- Genes, pol
- HIV
- HIV Antigens
- HIV Core Protein p24
- HIV Infections
- HIV Seropositivity
- HIV-1
- Humans
- In Vitro
- Longitudinal Studies
- T-Lymphocytes, Cytotoxic
- Vaccinia virus
- genetics
- immunology
Other ID:
UI: 102192424
From Meeting Abstracts