Enzensberger R, Doerr HW; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 653 (abstract no. C.569).
University Clinics Frankfurt, Department of Medical Virology, FRG
Testing for HIV antibodies is subject to continuous technical improvement. Therefore permanent monitoring of the tests quality is a major requirement in HIV serology. A panel of ten sera (three HIV-positive, one HIV-2 positive and six HIV negative) were sent to 77 German laboratories for a quality control test. Serum samples were assayed for HIV antibodies using 13 commercially available ELISAs (12 HIV 1-kits, 1 HIV 2-kit) IFA and Western blot. The study revealed a high standard of efficacy of the tests as well as an excellent interassay reproducibility. The overall sensitivity of HIV-1 ELISA was 99.5% (96.6% - 100%). By IFA and Western blot the same level of accuracy was achieved. With the exception of the specific HIV-2 ELISA, HIV 2 antibody were consistently recognized only by the Abbott recombinant EIA (48.9% cross-reactivity). Western blot was even more sensitive (78% reactivity) mostly due to the p24 protein band. In an additional series of 43 sera of weak reactivity in our laboratory (60% renal transplant recipients) Western blot and IFA were definitely more specific (91.2%/97%) than most ELISAs (18.9%-100%), emphasizing the importance of confirmatory testing for all equivocal results.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Blotting, Western
- Enzyme-Linked Immunosorbent Assay
- Evaluation Studies
- Germany, West
- HIV
- HIV Antibodies
- HIV Antigens
- HIV Core Protein p24
- HIV Infections
- HIV Seropositivity
- HIV-1
- HIV-2
- Immunoenzyme Techniques
- Mass Screening
- Sensitivity and Specificity
- immunology
- methods
Other ID:
UI: 102179357
From Meeting Abstracts