DONABEDIAN SM, PERRI MB, BOZIGAR PS, HERSHBERGER E, ANGULO FJ, ROSSITER S, JONES R, DEBESS E, JOHNSON S, PARK M, JOHNSON J, ZERVOS MJ; Interscience Conference on Antimicrobial Agents and Chemotherapy (41st : 2001 : Chicago, Ill.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2001 Dec 16-19; 41: abstract no. C2-1872.
William Beaumont Hospital, Royal Oak, MI
Three hundred and twenty quinupristin-dalfopristin (Q/D)-resistant (MICs 4-32 mcg/ml by broth microdilution) E. faecium were collected from humans, farm animals and food between 1999 and 2001 and evaluated by pulse-field gel electrophoresis (PFGE) and PCR to determine the presence of vat(D) and vat(E) resistance genes. Isolates were from 19 humans (4 hospitalized and 15 non-hospitalized) from six states (Michigan, Minnesota, Georgia, Oregon, Illinois, and Nevada), 276 fecal isolates from farm animals (4 beef cattle, 76 dairy cattle, 53 chicken, 68 swine and 75 turkey) from two states, and 25 food samples (3 pork and 22 chicken) purchased from grocery stores in five states. Human isolates did not share PFGE patterns with farm animal or food isolates. Two human isolates from Georgia were the same PFGE strain type with 2 bands difference using restriction enzyme smaI. Three isolates from retail poultry from Oregon were also the same strain type by PFGE. All isolates were negative for the vat(D) gene. Fourteen (26%) of the chicken farm isolates, 13 (17%) of the turkey farm isolates and 1dairy cattle isolate contained the vat(E) gene. The human, swine, beef, and retail food isolates were negative for vat(E) gene. These data suggest much diversity among Q/D-resistant E. faecium from humans, farm animals and food in grocery stores. Further work is necessary to determine the molecular mechanisms for Q/D-resistant E. faecium in the United States.
Publication Types:
Keywords:
- Animals
- Animals, Domestic
- Cattle
- Chickens
- Electrophoresis, Gel, Pulsed-Field
- Enterococcus faecium
- Feces
- Georgia
- Georgia (Republic)
- Humans
- Illinois
- Meat
- Michigan
- Microbial Sensitivity Tests
- Minnesota
- Nevada
- Oregon
- Swine
- Turkey
- Turkeys
- United States
- Virginiamycin
- quinupristin-dalfopristin
Other ID:
UI: 102269132
From Meeting Abstracts