MARIMON JM, GOMARIZ M, ZIGORRAGA C, CILLA G, PEREZ-TRALLERO E; Interscience Conference on Antimicrobial Agents and Chemotherapy.
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2000 Sep 17-20; 40: 94.
Complejo Hospitalario Donostia, San Sebastian, Spain
BACKGROUND: In our area, quinolone resistance in SNT from human isolates has been steadily increasing since 1994.METHODS: Quinolones susceptibility of SNT isolates from human and animal sources were studied by the disk diffusion and agar dilution methods (NCCLS guidelines). E. coli ATCC 25922 and S. aureus ATCC 29213 were included as controls. The presence of point mutations at codons 81, 83 and 87 of the gyrA gene were investigated by an allelic-specific PCR-RFLP assay as described by Giraud et al. (AAC 1999; 43: 2131-37).RESULTS: In 1999, nalidixic acid-resistant (NA-R) SNT strains reached the 26.0% (109/420). All NA-R strains showed a NA MIC 500 mg/L. In 1999 in a sample of 65 uncooked chickens bought from retail outlets we obtained 24 (37%) SNT and 9 of them (37.5%) were NA-R. All NA-R strains from human and animal origin had point mutations in the gyrA gene. Mutation at codon Asp-87 was the most frequently found in human (Table 1) and chicken products isolates (2/2 S. Enteritidis and 3/3 S. Hadar). No point mutation were found among the 8 S. Enteritidis, 4 S. Typhimurium, 2 S. Hadar and 1 S. Newport susceptible to NA studied. Table 1. SNT NA-R strains isolated from human stools with mutations in the gyrA gene [table: see text]. Conclusion: During 1999 we observed the highest levels of NA resistance in human SNT isolates. All NA-R strains, from human or animal origin, had point mutations in the gyrA gene.KEYWORDS: Antibiotic resistance; Salmonella
Publication Types:
Keywords:
- Animals
- Codon
- DNA Primers
- Humans
- Microbial Sensitivity Tests
- Mutation
- Nalidixic Acid
- Point Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Quinolones
- Salmonella
- Salmonella Infections
- Salmonella typhi
- Spain
- genetics
Other ID:
UI: 102248815
From Meeting Abstracts