Mutura C, Odawo L, Tukei P, Odoyo J, Marum L, Marum E, DeCock K, Chege J, Mburu M, Galgalo T, Baya I, Ngare C; International Conference on AIDS (15th : 2004 : Bangkok, Thailand).
Int Conf AIDS. 2004 Jul 11-16; 15: abstract no. MoPeB3153.
Centers for Disease Control & Prevention, Nairobi, Kenya
Background: KDHS+ 2003 included unlinked anonymous HIV testing. Mobile Voluntary Counseling and Testing (MVCT) sites were therefore established to provide counseling and testing to persons who wanted to learn their HIV results. Dried Blood Spots (DBS) were collected for quality control and assurance from every tenth MVCT client tested. Methods: Whole blood sampling was done on S&S 903 filter papers. The filter papers were dried overnight, packaged and sent to the laboratory at CDC-KEMRI for analysis. The DBS were assessed, eluted in buffer, and the elute tested for HIV antibodies using a standardized Enzygnost ELISA protocol. Rapid parallel testing using Determine and Unigold HIV test kits used in MVCT was also done. In case of discrepancy, InstantScreen was used as tiebreaker. Results: Out of 356 (one tenth of 3560) samples analyzed, only 2 (0.5%) showed discrepant results between lab and MVCT results. Both tested negative during MVCT in both tests, and positive in the laboratory runs. Conclusions: The study showed that the validity of HIV test results obtained in the field during the KDHS+ exercise under extremely adverse conditions were quite reliable. Validation is also practical using the S&S 903 filter papers and it is most reassuring and builds more confidence in the test results. MVCT services will therefore be useful as VCT scale up gets under way in remote Kenya.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Counseling
- Enzyme-Linked Immunosorbent Assay
- HIV Antibodies
- HIV Infections
- HIV Seropositivity
- Health Surveys
- Humans
- Kenya
Other ID:
UI: 102279457
From Meeting Abstracts