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Laboratory

Tobacco Methods

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Analyte(s) Product Type Instrumentation References
Nicotine/Nicotine Alkaloids All Tobacco Products Flow Analyzer 1
Capillary Electrophoresis 2
Total Reducing Sugars (Sucrose, Fructose, and Glucose) Cigars
Cigarettes
Roll-your-own
Pipe tobacco
Flow Analyzer 3
Ion Chromatography 4, 5
Reducing Sugars (Fructose and Glucose) Cigars
Cigarettes
Roll-your-own
Pipe tobacco
Flow Analyzer 6
Ion Chromatography 4, 5
Sieve Test Chewing tobacco
Snuff
Electric 8 inch sieve shaker, ROTAP (Reg. TM) 7
Product weight Cigars
Cigarettes
Environmental chamber
and analytical balance
8
  1. Astoria-Pacific Method A262 (contact Astoria-Pacific International for more information)
  2. Clarke, M.B., (2002) Quantitation of Nicotine in Tobacco Products by Capillary Electrophoresis J. AOAC Intl, Vol 85, No. 1
  3. Astoria-Pacific Method A250 (reproduced with permission from Astoria-Pacific International).
  4. Zook, C.M., Patel, P.M., LaCourse, W.R., Ralapati, S, (1996) Characterization of Tobacco Products by High-Performance Anion Exchange Chromatography-Pulsed Amperometric Detection, J. Agric. Food Chem., Vol 44, No. 7
  5. Dionex Technical Note 20, Analysis of Carbohydrates by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (2000), www.dionex.com
  6. Astoria-Pacific Procedure A041 (contact Astoria-Pacific International for more information)
  7. ATF Procedure 87-4, Testing Methods Used to Distinguish Between Chewing Tobacco and Snuff
  8. TTB directive to industry (pending)

Tobacco Links:

CORESTA - An association founded in 1956, ruled by French law, the purpose being to promote international cooperation in scientific research relative to tobacco.