Technical Abstract: Biosolids land application applies varying trace metal amounts to soils. Measuring total soil metals is typically performed to ensure environmental protection, yet this technique does not quantify which soil phases play important metal release or attenuation roles. We assessed the distribution of biosolids-borne Cd, Cr, Cu, Mo, Ni, and Zn associated with soluble/exchangeable, specifically adsorbed, easily reducible Fe/Mn oxides, Fe/Mn oxides and acid replaceable (i.e. organically complexed), residual organic, and residual inorganic phases. Biosolids were surface-applied (no incorporation) to experimental plots in 1991 at rates of 0, 2.5, 5, 10, 21, and 30 Mg/ha. Plots were split in half in 2002, with one-half receiving biosolids at rates identical to 1991 rates. In 2003, 0-8, 8-15, and 15-30-cm soil depths were collected and subjected to 4 M HNO3 digestion and sequential fractionation. The 4 M HNO3 extraction showed increasing biosolids application rates increased Cr content, while repeated application increased Cr and Ni as compared to the single application in the 0-8-cm depth. The 4 M HNO3 extraction suggested downward Cu transport, while Cr and Zn were immobile. The sequential extraction procedure, more sensitive to changes in soil metal pools, suggested that repeated biosolids application increased the downward transport potential of easily reducible Fe/Mn oxide-bound Cd, Cu associated with specifically adsorbed and residual organic fractions, residual inorganic Mo, and soluble/exchangeable Ni. Within the 15-30-cm depth, increasing biosolids application, regardless of time applied, increased Cu associated with specifically adsorbed, Fe/Mn oxides (i.e. crystalline) and acid replaceable, and residual organic phases, and Zn associated with the Fe/Mn oxides and acid replaceable phase. The soil studied was fine-loamy over sandy/sandy-skeletal, thus the texture likely facilitated downward colloidal-associated Cd, Cu, Mo, and Zn transport. On coarse-textured semi-arid soils, biosolids application rates should match the plant N needs to avoid downward trace metal transport.