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Research Project:
Investigation of Seedling Yellows Cross Protection by Mild Components of Dekopon Citrus Tristeza Virus Strain
Location: Crop Diseases, Pests and Genetics
Project Number: 5302-22000-009-02
Project Type:
Trust
Start Date: Nov 01, 2008
End Date: Oct 31, 2009
Objective:
1) Develop qPCR probes for the different Dekopon sub-isolates and quantify each in single and mixed infections to assess interference in accumulation/replication of each sub-isolate.
2) Examine P20, P23, and CP (suppressors of gene silencing) for each sub-isolate for genetic variation and associate with symptom expression.
3) Characterize siRNA profiles in mixed and single infected plants.
Approach:
Aphid transmission, reverse transcription (RT) polymerase chain reaction (PCR), real time PCR (qPCR), cloning, sequencing, western blots, northern blots, hybridizations. Documents Reimbursable with Citrus Research Board. Log 37119.
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Last Modified: 05/08/2009
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