Technical Abstract: Campylobacter jejuni is one of the most important causes of human diarrhea worldwide. In the present work, multilocus sequence typing (MLST) was used to study the genotypic diversity of 145 C. jejuni isolates from 135 chicken meat preparations sampled across Belgium. Isolates were further typed by pulsed-field gel electrophoresis (PFGE) and their susceptibility to 6 antimicrobials was determined. Fifty-seven sequence types (STs) were identified; 26.8% of the total typed isolates were ST-50, ST-45, or ST-257, of the clonal complexes CC-21, CC-45 and CC-257, respectively. One clonal group contained 22% (32/145) of all isolates, originated from five different companies and was isolated over 7 sampling months. Additionally, 53.1% of C. jejuni isolates were resistant to ciprofloxacin, and 48.2% were resistant to tetracycline; 42 isolates (28.9%) were resistant to both ciprofloxacin and tetracycline. Correlation between certain C. jejuni clonal groups and resistance to ciprofloxacin and tetracycline was notable. C. jejuni isolates assigned to CC-21 (n = 35) were frequently resistant to ciprofloxacin (65.7%) and tetracycline (40%); however, 87.5% (21/24) of the isolates assigned to CC-45 were pansusceptible. The present study demonstrates that certain C. jejuni genotypes are frequently recurrent in the chicken meat supply. Results from molecular typing, combined with data on sample sources, indicate a possible dissemination of C. jejuni clones characterised with high resistance to ciprofloxacin and/or tetracycline. Whether certain clonal groups are common in the environment and repeatedly infect Belgian broiler flocks or whether they have the potential to persist on farms or in slaughterhouses needs further investigation.