U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

R75
Sonicated Calf-Thymus or Salmon-Sperm DNA

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DNA 1 g
Distilled water 1 liter

Stir 3-4 h to dissolve. May be heated to 60°C. Sonicate until MW is about 300,000 - 500,000 daltons. Store frozen in 1 ml aliquots.




Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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