U.S. Food & Drug Administration
Center for
Food
Safety &
Applied
Nutrition
Bacteriological
Analytical
Manual Online
January 2001
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R16
Crystal Violet Stain
(for Bacteria)
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1. Crystal violet in dilute alcohol
Crystal violet (90% dye content) |
2 g |
Ethanol (95%) |
20 ml |
Distilled water |
80 ml |
2. Ammonium oxalate crystal violet (Hucker's [See R32])
Either solution is generally considered suitable as a simple stain to observe morphology.
Hypertext Source: Bacteriological Analytical
Manual, 8th Edition, Revision A, 1998.
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Hypertext updated by rim 2000-MAR-31