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R11
Butterfield's Phosphate-Buffered Dilution Water
KH2PO4 | 34 g |
Distilled water | 500 ml |
Adjust pH to 7.2 with 1 N NaOH. Bring volume to 1 liter with distilled water. Sterilize 15 min at 121°C. Store in refrigerator.
Take 1.25 ml of above stock solution and bring volume to 1 liter with distilled water. Dispense into bottles to 90 or 99 ± 1 ml. Sterilize 15 min at 121°C.
Hypertext updated by rim 2000-MAR-28