Seafood Products Research Center Center for Food Safety & Applied Nutrition Regulatory Fish Encyclopedia: IEF Gel Lane Banding Pattern Peak Report Data |
Gel Image | Fish Image | Calibrated Gel | Comparison Gel |
Lane a pI Markers |
Lane b Co-Migrated |
Lane c Specimen |
Lane d pI Markers |
Lane e Co-Migrated |
Lane f Specimen |
Lane g pI Markers |
Go to Gel Schematic |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 3.5 | 31 | 2.7 | 0.278006 | 11 | 5.11676 | 14.1 | 10.7 | 3.5 | 32 | 10.7 | 0.26946 |
2 | 4.55 | 185 | 16.4 | 0.224064 | 9 | 3.09101 | 8.5 | 6.5 | 4.55 | 183.1 | 8.9 | 0.196791 |
3 | 5.2 | 261 | 23.1 | 0.33924 | 7 | 4.16711 | 11.5 | 8.7 | 5.2 | 259.1 | 7.2 | 0.327497 |
4 | 5.694 | 369 | 32.7 | 0.060328 | 5 | 0.468972 | 1.3 | 1 | 5.694 | 368.1 | 4.6 | 0.057839 |
5 | 5.85 | 403 | 35.7 | 0.524856 | 4 | 3.49237 | 9.6 | 7.3 | 5.85 | 403.1 | 3.8 | 0.523288 |
6 | 6.55 | 569 | 50.4 | 0.343854 | 5 | 3.06984 | 8.5 | 6.4 | 6.55 | 568.6 | 5.3 | 0.328357 |
7 | 6.85 | 609 | 53.9 | 0.163664 | 8 | 1.91181 | 5.3 | 4 | 6.85 | 609.2 | 7.5 | 0.143737 |
8 | 7.35 | 705 | 62.4 | 0.304051 | 6 | 3.15783 | 8.7 | 6.6 | 7.35 | 703.8 | 6 | 0.29492 |
9 | 8.15 | 869 | 76.9 | 0.101052 | 10 | 1.72133 | 4.7 | 3.6 | 8.15 | 868.1 | 10.1 | 0.0960746 |
10 | 8.45 | 932 | 82.5 | 0.155428 | 7 | 1.85125 | 5.1 | 3.9 | 8.45 | 932.4 | 6.8 | 0.153388 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 8.65 | 975 | 86.3 | 0.147068 | 5 | 1.386 | 3.8 | 2.9 | 8.65 | 975.2 | 5.5 | 0.143398 |
12 | 9.3 | 1092 | 96.6 | 0.494924 | 8 | 6.84826 | 18.9 | 14.4 | 9.3 | 1089.2 | 8.4 | 0.45914 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 3.5 | 30 | 2.6 | 0.201063 | 9 | 3.12341 | 5.1 | 4.3 | 3.5 | 31.4 | 9.4 | 0.187368 |
2 | 4.172 | 126 | 11.1 | 0.587814 | 4 | 4.34474 | 7.1 | 6 | 4.172 | 126.4 | 4 | 0.610832 |
3 | 4.319 | 147 | 13 | 0.404284 | 4 | 2.60735 | 4.3 | 3.6 | 4.319 | 147 | 3.6 | 0.40789 |
4 | 4.445 | 165 | 14.6 | 0.0203725 | 4 | 0.140637 | 0.2 | 0.2 | 4.445 | 164.8 | 4 | 0.020025 |
5 | 4.55 | 180 | 15.9 | 0.214985 | 4 | 1.487 | 2.4 | 2 | 4.55 | 180 | 4.3 | 0.195822 |
6 | 4.819 | 211 | 18.6 | 0.116748 | 3 | 0.576937 | 0.9 | 0.8 | 4.819 | 211.6 | 2.8 | 0.115028 |
7 | 5.018 | 234 | 20.7 | 0.0780556 | 6 | 0.70297 | 1.2 | 1 | 5.018 | 232.2 | 6.5 | 0.0614543 |
8 | 5.113 | 245 | 21.6 | 0.0637594 | 3 | 0.292214 | 0.5 | 0.4 | 5.113 | 245.6 | 3 | 0.0544673 |
9 | 5.2 | 255 | 22.5 | 0.19205 | 3 | 1.14468 | 1.9 | 1.6 | 5.2 | 255 | 3.3 | 0.196378 |
10 | 5.25 | 266 | 23.5 | 0.394546 | 5 | 3.21001 | 5.3 | 4.4 | 5.25 | 266.5 | 4.6 | 0.395342 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 5.385 | 296 | 26.1 | 0.216639 | 4 | 1.45917 | 2.4 | 2 | 5.385 | 295.9 | 3.9 | 0.213408 |
12 | 5.493 | 320 | 28.2 | 0.0137639 | 3 | 0.0756936 | 0.1 | 0.1 | 5.493 | 321.1 | 2.9 | 0.0149301 |
13 | 5.539 | 330 | 29.1 | 0.0427229 | 2 | 0.169218 | 0.3 | 0.2 | 5.539 | 329.9 | 2.2 | 0.0430821 |
14 | 5.593 | 342 | 30.2 | 0.0217843 | 3 | 0.124517 | 0.2 | 0.2 | 5.593 | 341.7 | 3.4 | 0.0206002 |
15 | 5.674 | 360 | 31.8 | 0.413424 | 4 | 2.70263 | 4.4 | 3.7 | 5.674 | 360.4 | 3.7 | 0.407949 |
16 | 5.746 | 376 | 33.2 | 0.446222 | 4 | 3.04642 | 5 | 4.2 | 5.746 | 376.7 | 4 | 0.433786 |
17 | 5.85 | 399 | 35.2 | 0.329774 | 6 | 3.55216 | 5.8 | 4.9 | 5.85 | 398.8 | 6 | 0.333609 |
18 | 5.942 | 421 | 37.2 | 0.0266311 | 4 | 0.2844 | 0.5 | 0.4 | 5.942 | 422.4 | 4.3 | 0.0369504 |
19 | 5.996 | 434 | 38.3 | 0.117765 | 6 | 1.18811 | 2 | 1.6 | 5.996 | 435 | 6.3 | 0.106821 |
20 | 6.15 | 471 | 41.6 | 0.097608 | 3 | 0.451074 | 0.7 | 0.6 | 6.15 | 471.1 | 2.6 | 0.0960616 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
21 | 6.188 | 480 | 42.4 | 0.184597 | 2 | 0.798576 | 1.3 | 1.1 | 6.188 | 479.9 | 2.4 | 0.184025 |
22 | 6.258 | 497 | 43.9 | 0.193977 | 5 | 1.55368 | 2.6 | 2.1 | 6.258 | 499.5 | 5.4 | 0.163607 |
23 | 6.308 | 509 | 44.9 | 0.486009 | 3 | 2.65332 | 4.4 | 3.7 | 6.308 | 508.5 | 3.2 | 0.472721 |
24 | 6.463 | 546 | 48.2 | 0.0986944 | 14 | 2.19128 | 3.6 | 3 | 6.463 | 547.8 | 13.6 | 0.0911641 |
25 | 6.55 | 567 | 50 | 0.145682 | 6 | 1.45887 | 2.4 | 2 | 6.55 | 565.2 | 6.3 | 0.131037 |
26 | 6.85 | 607 | 53.6 | 0.128366 | 7 | 1.2529 | 2.1 | 1.7 | 6.85 | 607.2 | 6.5 | 0.108565 |
27 | 7.35 | 701 | 61.9 | 0.306378 | 5 | 2.56969 | 4.2 | 3.5 | 7.35 | 700.5 | 4.9 | 0.29866 |
28 | 7.873 | 807 | 71.2 | 0.0487105 | 6 | 0.498262 | 0.8 | 0.7 | 7.873 | 805.8 | 6.1 | 0.0458547 |
29 | 8.15 | 863 | 76.2 | 0.0727012 | 10 | 4.41182 | 7.3 | 6.1 | 8.15 | 872.7 | 9.7 | 0.257824 |
30 | 8.45 | 925 | 81.6 | 0.10481 | 6 | 1.17059 | 1.9 | 1.6 | 8.45 | 925.7 | 6.4 | 0.103947 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
31 | 8.65 | 971 | 85.7 | 0.230475 | 18 | 6.48603 | 10.7 | 8.9 | 8.65 | 973.9 | 17.9 | 0.203936 |
32 | 9.3 | 1091 | 96.3 | 0.387995 | 8 | 5.10592 | 8.4 | 7 | 9.3 | 1088.2 | 8.2 | 0.351806 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 4.129 | 117 | 10.3 | 0.600755 | 4 | 4.36283 | 12.1 | 10 | 4.129 | 117.4 | 3.9 | 0.625725 |
2 | 4.276 | 137 | 12.1 | 0.396259 | 4 | 2.56389 | 7.1 | 5.9 | 4.276 | 137.6 | 3.5 | 0.409537 |
3 | 4.473 | 164 | 14.5 | 0.0244583 | 4 | 0.144251 | 0.4 | 0.3 | 4.473 | 165.7 | 3.6 | 0.0226887 |
4 | 4.608 | 181 | 16 | 0.0261647 | 7 | 0.319687 | 0.9 | 0.7 | 4.608 | 178.2 | 6.8 | 0.026378 |
5 | 4.761 | 198 | 17.5 | 0.163525 | 3 | 0.764797 | 2.1 | 1.8 | 4.761 | 198.3 | 2.8 | 0.155074 |
6 | 4.967 | 221 | 19.5 | 0.0855019 | 6 | 0.847929 | 2.4 | 1.9 | 4.967 | 219.3 | 6.2 | 0.0774499 |
7 | 5.057 | 231 | 20.4 | 0.093121 | 3 | 0.44265 | 1.2 | 1 | 5.057 | 231.2 | 2.6 | 0.0957225 |
8 | 5.146 | 241 | 21.3 | 0.0737522 | 2 | 0.295516 | 0.8 | 0.7 | 5.146 | 240.9 | 2.3 | 0.0739083 |
9 | 5.209 | 249 | 22 | 0.514007 | 3 | 2.42981 | 6.8 | 5.6 | 5.209 | 249.5 | 2.9 | 0.46757 |
10 | 5.287 | 266 | 23.5 | 0.0598955 | 9 | 1.6171 | 4.5 | 3.7 | 5.287 | 256.6 | 9.4 | 0.0972546 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 5.347 | 279 | 24.6 | 0.283228 | 3 | 1.55288 | 4.3 | 3.6 | 5.347 | 279.4 | 3.1 | 0.282468 |
12 | 5.466 | 305 | 26.9 | 0.0217393 | 2 | 0.0944062 | 0.3 | 0.2 | 5.466 | 305.3 | 2.5 | 0.0213196 |
13 | 5.508 | 314 | 27.7 | 0.0634894 | 2 | 0.227347 | 0.6 | 0.5 | 5.508 | 314 | 2.3 | 0.0555346 |
14 | 5.549 | 323 | 28.5 | 0.0399375 | 7 | 0.532152 | 1.5 | 1.2 | 5.549 | 323.4 | 7.3 | 0.0409555 |
15 | 5.641 | 343 | 30.3 | 0.510983 | 3 | 2.57559 | 7.2 | 5.9 | 5.641 | 343 | 2.9 | 0.503587 |
16 | 5.714 | 359 | 31.7 | 0.509518 | 4 | 3.13079 | 8.7 | 7.2 | 5.714 | 359.1 | 3.6 | 0.494108 |
17 | 5.82 | 382 | 33.7 | 0.420526 | 4 | 2.50799 | 7 | 5.8 | 5.82 | 382.6 | 3.6 | 0.39617 |
18 | 5.915 | 404 | 35.7 | 0.0280886 | 5 | 0.256411 | 0.7 | 0.6 | 5.915 | 402.1 | 5 | 0.0290468 |
19 | 5.961 | 415 | 36.7 | 0.117287 | 6 | 1.10975 | 3.1 | 2.5 | 5.961 | 415.6 | 5.7 | 0.110649 |
20 | 6.111 | 451 | 39.8 | 0.0829036 | 3 | 0.479527 | 1.3 | 1.1 | 6.111 | 450.7 | 3.2 | 0.0835196 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
21 | 6.145 | 459 | 40.5 | 0.144163 | 4 | 1.08389 | 3 | 2.5 | 6.145 | 459.3 | 4.1 | 0.147691 |
22 | 6.232 | 480 | 42.4 | 0.0827561 | 3 | 0.935735 | 2.6 | 2.1 | 6.232 | 482.9 | 3.5 | 0.152521 |
23 | 6.278 | 491 | 43.4 | 0.421403 | 6 | 4.33235 | 12 | 9.9 | 6.278 | 490 | 6 | 0.409312 |
24 | 6.433 | 528 | 46.6 | 0.1114 | 9 | 1.63581 | 4.5 | 3.8 | 6.433 | 527.5 | 9 | 0.102267 |
25 | 6.79 | 586 | 51.8 | 0.0155837 | 18 | 0.514722 | 1.4 | 1.2 | 6.79 | 593 | 18 | 0.0161337 |
26 | 6.942 | 611 | 54 | 0.0245853 | 6 | 0.173608 | 0.5 | 0.4 | 6.942 | 612.2 | 6.5 | 0.0151228 |
27 | 7.815 | 785 | 69.3 | 0.0432651 | 8 | 0.593712 | 1.6 | 1.4 | 7.815 | 787.1 | 8.2 | 0.0410231 |
28 | 8.613 | 958 | 84.6 | 0.0339251 | 7 | 0.469251 | 1.3 | 1.1 | 8.613 | 959 | 7.5 | 0.0354924 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 3.5 | 31 | 2.7 | 0.211559 | 7 | 2.61333 | 9.7 | 7 | 3.5 | 32 | 7 | 0.209572 |
2 | 4.55 | 168 | 14.9 | 0.31748 | 5 | 2.28931 | 8.5 | 6.1 | 4.55 | 167.7 | 4.6 | 0.283451 |
3 | 5.2 | 238 | 21.1 | 0.4036 | 5 | 3.22535 | 11.9 | 8.6 | 5.2 | 238.4 | 4.6 | 0.399542 |
4 | 5.85 | 377 | 33.4 | 0.544934 | 3 | 3.17967 | 11.8 | 8.5 | 5.85 | 376.8 | 3.2 | 0.557453 |
5 | 6.55 | 544 | 48.1 | 0.378792 | 4 | 2.87696 | 10.7 | 7.7 | 6.55 | 543.5 | 4.4 | 0.372618 |
6 | 6.85 | 579 | 51.2 | 0.144641 | 4 | 1.02522 | 3.8 | 2.7 | 6.85 | 579.6 | 4.3 | 0.133973 |
7 | 7.35 | 675 | 59.7 | 0.27368 | 6 | 2.73707 | 10.1 | 7.3 | 7.35 | 674.2 | 5.8 | 0.267265 |
8 | 8.15 | 845 | 74.8 | 0.107299 | 10 | 1.77695 | 6.6 | 4.8 | 8.15 | 846.2 | 9.8 | 0.10214 |
9 | 8.45 | 916 | 81.1 | 0.133952 | 6 | 1.35607 | 5 | 3.6 | 8.45 | 916 | 5.8 | 0.132547 |
10 | 8.65 | 961 | 85 | 0.133018 | 5 | 1.25237 | 4.6 | 3.4 | 8.65 | 960.5 | 5.4 | 0.129883 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 9.3 | 1090 | 96.5 | 0.380001 | 8 | 4.67847 | 17.3 | 12.5 | 9.3 | 1087.4 | 7.6 | 0.34722 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 3.5 | 37 | 3.3 | 0.206288 | 9 | 3.21341 | 5.9 | 4.9 | 3.5 | 38.4 | 9.4 | 0.192812 |
2 | 4.162 | 124 | 11 | 0.521363 | 4 | 4.05894 | 7.5 | 6.2 | 4.162 | 124.5 | 4.3 | 0.533401 |
3 | 4.314 | 144 | 12.7 | 0.310688 | 4 | 2.29077 | 4.2 | 3.5 | 4.314 | 144.5 | 4.1 | 0.315127 |
4 | 4.451 | 162 | 14.3 | 0.034381 | 5 | 0.283412 | 0.5 | 0.4 | 4.451 | 162.2 | 5 | 0.0322658 |
5 | 4.55 | 175 | 15.5 | 0.146466 | 5 | 1.32127 | 2.4 | 2 | 4.55 | 175 | 5.3 | 0.140346 |
6 | 4.692 | 191 | 16.9 | 0.0179075 | 7 | 0.519868 | 1 | 0.8 | 4.692 | 184.3 | 7.5 | 0.0393217 |
7 | 4.835 | 207 | 18.3 | 0.0760325 | 4 | 0.548075 | 1 | 0.8 | 4.835 | 206.3 | 3.9 | 0.0787952 |
8 | 5.004 | 226 | 20 | 0.0781509 | 7 | 1.00596 | 1.9 | 1.5 | 5.004 | 225.9 | 7.3 | 0.0780244 |
9 | 5.129 | 240 | 21.2 | 0.0864975 | 5 | 0.727585 | 1.3 | 1.1 | 5.129 | 239.1 | 5.1 | 0.08109 |
10 | 5.2 | 248 | 21.9 | 0.183286 | 5 | 1.44733 | 2.7 | 2.2 | 5.2 | 248.1 | 4.6 | 0.178519 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 5.251 | 259 | 22.9 | 0.326361 | 5 | 2.5505 | 4.7 | 3.9 | 5.251 | 258.6 | 4.5 | 0.316658 |
12 | 5.329 | 276 | 24.4 | 0.0205936 | 9 | 0.55428 | 1 | 0.8 | 5.329 | 267.8 | 8.6 | 0.0361879 |
13 | 5.394 | 290 | 25.7 | 0.122498 | 5 | 1.05533 | 1.9 | 1.6 | 5.394 | 290 | 4.8 | 0.124212 |
14 | 5.532 | 320 | 28.3 | 0.0150984 | 4 | 0.0625809 | 0.1 | 0.1 | 5.532 | 322.6 | 3.5 | 0.0100188 |
15 | 5.55 | 324 | 28.7 | 0.0260118 | 8 | 0.335433 | 0.6 | 0.5 | 5.55 | 328.3 | 8.3 | 0.022794 |
16 | 5.61 | 337 | 29.8 | 0.0168113 | 5 | 0.0880743 | 0.2 | 0.1 | 5.61 | 335.4 | 4.7 | 0.0106561 |
17 | 5.67 | 350 | 31 | 0.301174 | 3 | 1.01863 | 1.9 | 1.6 | 5.67 | 349.6 | 3 | 0.193491 |
18 | 5.716 | 360 | 31.9 | 0.380274 | 8 | 5.39953 | 10 | 8.2 | 5.716 | 359.1 | 8.4 | 0.361436 |
19 | 5.85 | 389 | 34.4 | 0.302295 | 5 | 2.6888 | 5 | 4.1 | 5.85 | 389.3 | 5.1 | 0.299588 |
20 | 5.921 | 405 | 35.8 | 0.00332911 | 1 | 0.00105652 | 0 | 0 | 5.921 | 404 | 0.5 | 0.00101894 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
21 | 5.961 | 414 | 36.6 | 0.0167785 | 4 | 0.107463 | 0.2 | 0.2 | 5.961 | 415.1 | 4.1 | 0.0149598 |
22 | 6.149 | 456 | 40.4 | 0.0395402 | 4 | 0.312382 | 0.6 | 0.5 | 6.149 | 456.9 | 4.2 | 0.042278 |
23 | 6.207 | 469 | 41.5 | 0.0778071 | 5 | 0.678948 | 1.3 | 1 | 6.207 | 469.6 | 5.1 | 0.0743869 |
24 | 6.282 | 486 | 43 | 0.064533 | 3 | 0.309421 | 0.6 | 0.5 | 6.282 | 483.5 | 2.6 | 0.0659419 |
25 | 6.305 | 491 | 43.5 | 0.362562 | 4 | 2.49498 | 4.6 | 3.8 | 6.305 | 491.7 | 3.8 | 0.36666 |
26 | 6.465 | 527 | 46.6 | 0.0395087 | 11 | 0.92055 | 1.7 | 1.4 | 6.465 | 531.5 | 11.2 | 0.0461949 |
27 | 6.55 | 546 | 48.3 | 0.160627 | 6 | 1.60039 | 3 | 2.4 | 6.55 | 545.3 | 6.1 | 0.146995 |
28 | 6.85 | 588 | 52 | 0.109717 | 6 | 1.0402 | 1.9 | 1.6 | 6.85 | 586.8 | 6.3 | 0.0931009 |
29 | 6.987 | 613 | 54.2 | 0.015423 | 18 | 0.671363 | 1.2 | 1 | 6.987 | 599.5 | 18 | 0.0210435 |
30 | 7.213 | 654 | 57.9 | 0.0168084 | 13 | 0.692524 | 1.3 | 1.1 | 7.213 | 667.9 | 13.4 | 0.0291196 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
31 | 7.35 | 679 | 60.1 | 0.28277 | 5 | 2.25844 | 4.2 | 3.4 | 7.35 | 679 | 4.8 | 0.265943 |
32 | 7.89 | 789 | 69.8 | 0.0432103 | 6 | 0.467843 | 0.9 | 0.7 | 7.89 | 787.5 | 6.5 | 0.0408929 |
33 | 8.15 | 842 | 74.5 | 0.0689575 | 8 | 0.931159 | 1.7 | 1.4 | 8.15 | 841.6 | 8 | 0.0657459 |
34 | 8.45 | 908 | 80.4 | 0.097366 | 6 | 1.09788 | 2 | 1.7 | 8.45 | 909.2 | 6.4 | 0.0963312 |
35 | 8.65 | 958 | 84.8 | 0.209762 | 18 | 6.19469 | 11.4 | 9.5 | 8.65 | 961.6 | 18 | 0.194169 |
36 | 9.3 | 1087 | 96.2 | 0.409403 | 8 | 5.27743 | 9.7 | 8.1 | 9.3 | 1084.4 | 7.8 | 0.379586 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 3.558 | 48 | 4.2 | 0.00950801 | 2 | 0.0268453 | 0.1 | 0.1 | 3.558 | 48.7 | 1.6 | 0.00929596 |
2 | 4.168 | 127 | 11.2 | 0.639978 | 4 | 4.81499 | 14.3 | 12 | 4.168 | 127.3 | 4.1 | 0.666643 |
3 | 4.322 | 147 | 13 | 0.460561 | 4 | 2.95634 | 8.8 | 7.4 | 4.322 | 147 | 3.6 | 0.468262 |
4 | 4.438 | 162 | 14.3 | 0.0136955 | 3 | 0.0576938 | 0.2 | 0.1 | 4.438 | 161.9 | 2.6 | 0.0125857 |
5 | 4.538 | 175 | 15.5 | 0.0341815 | 3 | 0.162135 | 0.5 | 0.4 | 4.538 | 174.9 | 3.1 | 0.0290896 |
6 | 4.677 | 191 | 16.9 | 0.0218645 | 9 | 0.373721 | 1.1 | 0.9 | 4.677 | 186.7 | 9 | 0.0234638 |
7 | 4.835 | 209 | 18.5 | 0.157295 | 3 | 0.763043 | 2.3 | 1.9 | 4.835 | 208.8 | 2.7 | 0.156911 |
8 | 5.02 | 230 | 20.4 | 0.104107 | 5 | 0.927383 | 2.8 | 2.3 | 5.02 | 228.2 | 5.4 | 0.0976666 |
9 | 5.117 | 241 | 21.3 | 0.135192 | 2 | 0.530279 | 1.6 | 1.3 | 5.117 | 240.6 | 2.3 | 0.130741 |
10 | 5.207 | 252 | 22.3 | 0.105402 | 3 | 0.65616 | 1.9 | 1.6 | 5.207 | 251.6 | 3.4 | 0.108576 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 5.247 | 261 | 23.1 | 0.559636 | 3 | 2.4123 | 7.2 | 6 | 5.247 | 260.8 | 2.5 | 0.537626 |
12 | 5.287 | 270 | 23.9 | 0.0654356 | 6 | 0.972397 | 2.9 | 2.4 | 5.287 | 266.6 | 6.4 | 0.0850985 |
13 | 5.323 | 278 | 24.6 | 0.0518156 | 3 | 0.299156 | 0.9 | 0.7 | 5.323 | 277.1 | 3.4 | 0.0499641 |
14 | 5.394 | 294 | 26 | 0.21629 | 5 | 1.92429 | 5.7 | 4.8 | 5.394 | 294.5 | 4.8 | 0.22537 |
15 | 5.546 | 328 | 29 | 0.0431628 | 6 | 0.505397 | 1.5 | 1.3 | 5.546 | 328.7 | 6.4 | 0.0445937 |
16 | 5.595 | 339 | 30 | 0.0553444 | 3 | 0.300384 | 0.9 | 0.7 | 5.595 | 339 | 3.5 | 0.0487631 |
17 | 5.662 | 354 | 31.3 | 0.326401 | 3 | 1.42316 | 4.2 | 3.5 | 5.662 | 354.1 | 2.6 | 0.311483 |
18 | 5.712 | 365 | 32.3 | 0.375423 | 6 | 4.3189 | 12.8 | 10.8 | 5.712 | 364.7 | 6.3 | 0.384944 |
19 | 5.77 | 378 | 33.5 | 0.0782222 | 3 | 0.386513 | 1.1 | 1 | 5.77 | 377.9 | 3 | 0.0723974 |
20 | 5.837 | 393 | 34.8 | 0.317635 | 3 | 1.69365 | 5 | 4.2 | 5.837 | 392.9 | 3 | 0.316256 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
21 | 5.912 | 410 | 36.3 | 0.0285248 | 3 | 0.149318 | 0.4 | 0.4 | 5.912 | 409.3 | 2.9 | 0.0290604 |
22 | 5.956 | 420 | 37.2 | 0.0685895 | 3 | 0.329381 | 1 | 0.8 | 5.956 | 420.3 | 2.8 | 0.0674436 |
23 | 6.145 | 463 | 41 | 0.0794699 | 3 | 0.419551 | 1.2 | 1 | 6.145 | 463.1 | 3 | 0.0779455 |
24 | 6.198 | 475 | 42 | 0.15203 | 3 | 0.928853 | 2.8 | 2.3 | 6.198 | 475.5 | 3.4 | 0.153991 |
25 | 6.277 | 493 | 43.6 | 0.121032 | 4 | 0.722183 | 2.1 | 1.8 | 6.277 | 492.6 | 3.6 | 0.113692 |
26 | 6.303 | 499 | 44.2 | 0.628692 | 2 | 2.80881 | 8.3 | 7 | 6.303 | 498.9 | 2.4 | 0.648685 |
27 | 6.392 | 519 | 45.9 | 0.0141625 | 5 | 0.109801 | 0.3 | 0.3 | 6.392 | 518.5 | 4.7 | 0.0130674 |
28 | 6.466 | 536 | 47.4 | 0.0334532 | 8 | 0.422632 | 1.3 | 1.1 | 6.466 | 536 | 7.6 | 0.031397 |
29 | 6.82 | 591 | 52.3 | 0.0167854 | 11 | 0.274283 | 0.8 | 0.7 | 6.82 | 587.9 | 11 | 0.0141137 |
30 | 6.958 | 615 | 54.4 | 0.0226335 | 7 | 0.253385 | 0.8 | 0.6 | 6.958 | 615.8 | 6.6 | 0.0215345 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
31 | 7.847 | 790 | 69.9 | 0.0714622 | 5 | 0.643991 | 1.9 | 1.6 | 7.847 | 789.3 | 5.4 | 0.0668086 |
32 | 8.577 | 946 | 83.7 | 0.0608492 | 11 | 1.14446 | 3.4 | 2.9 | 8.577 | 946.8 | 10.8 | 0.0598252 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 3.5 | 44 | 3.9 | 0.295535 | 10 | 5.1013 | 14.4 | 10.7 | 3.5 | 46.3 | 10.5 | 0.275118 |
2 | 4.55 | 178 | 15.8 | 0.255211 | 7 | 2.94472 | 8.3 | 6.2 | 4.55 | 179.9 | 6.9 | 0.23929 |
3 | 5.2 | 253 | 22.4 | 0.321945 | 7 | 3.87595 | 10.9 | 8.2 | 5.2 | 253.6 | 7 | 0.312119 |
4 | 5.85 | 403 | 35.7 | 0.446865 | 5 | 3.67658 | 10.3 | 7.7 | 5.85 | 402.8 | 4.6 | 0.4533 |
5 | 6.55 | 564 | 49.9 | 0.397994 | 5 | 3.02459 | 8.5 | 6.4 | 6.55 | 564 | 4.5 | 0.376425 |
6 | 6.85 | 602 | 53.3 | 0.241551 | 6 | 2.23274 | 6.3 | 4.7 | 6.85 | 602.7 | 5.7 | 0.220914 |
7 | 7.35 | 696 | 61.6 | 0.301419 | 6 | 2.96465 | 8.3 | 6.2 | 7.35 | 695.2 | 6 | 0.278198 |
8 | 8.15 | 863 | 76.4 | 0.0904277 | 10 | 1.55243 | 4.4 | 3.3 | 8.15 | 862.3 | 10 | 0.0876131 |
9 | 8.45 | 925 | 81.9 | 0.168303 | 7 | 2.05528 | 5.8 | 4.3 | 8.45 | 925 | 7.1 | 0.164008 |
10 | 8.65 | 968 | 85.7 | 0.121913 | 7 | 1.50364 | 4.2 | 3.2 | 8.65 | 967.2 | 7.1 | 0.119274 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 9.3 | 1089 | 96.4 | 0.449244 | 9 | 6.60263 | 18.6 | 13.9 | 9.3 | 1085.5 | 8.9 | 0.41959 |
a | b | c | d | e | f | g | ||
MK | CO | SP | MK | CO | SP | MK | ||
Key to Gel Schematic: | MK=pI Marker Lanes, pI bands are in red SP=Specimen Lanes, Specimen bands are in blue CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue) Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen band is reported when overlapped peak position deviates slightly from nominal pI Marker position. Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness. Return to Top of Gel Schematic |
DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 3 PeakCount: 12 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 4 PeakCount: 32 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 5 PeakCount: 28 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 6 PeakCount: 11 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 7 PeakCount: 36 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 8 PeakCount: 32 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 9 PeakCount: 11 Return to Lane Navigator |
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