RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Blue Catfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Ictalurus furcatus
FDA Seafood List Common Name: Blue Catfish
FDA Seafood List Market Name: Catfish
Unique IEF Gel Code: 19970206gel03
Unique Web Code: bch01Srr
Tissue Aliquot Code: ictafurc L95-004 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	110	12.7	0.0760942	4	0.537912	6.9	4.8	4.55	110.2	4.1	0.0738094
2	5.2	199	23	0.0800305	5	0.673175	8.7	6	5.2	199.3	4.9	0.0772274
3	5.85	317	36.6	0.166008	4	1.07252	13.9	9.5	5.85	317.2	3.6	0.16793
4	6.55	464	53.6	0.124965	6	1.3205	17.1	11.7	6.55	463.3	6.4	0.11691
5	6.85	494	57	0.0476673	5	0.37562	4.9	3.3	6.85	494.9	4.6	0.0460035
6	7.35	558	64.4	0.0748809	5	0.590784	7.6	5.2	7.35	557.7	4.7	0.0709529
7	8.15	687	79.3	0.0787998	8	1.08478	14	9.6	8.15	687.2	7.9	0.0774248
8	8.45	755	87.2	0.124688	6	1.40274	18.1	12.4	8.45	754.7	6.5	0.122037
9	8.65	804	92.8	0.0904149	4	0.649253	8.4	5.7	8.65	803.9	4.2	0.0873292
10	9.3	850	98.2	0.00990397	2	0.0327682	0.4	0.3	9.3	849.6	1.8	0.0100701

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.925	23	2.7	0.214728	8	3.2947	15.2	11.6	3.925	23.2	8.4	0.222124
2	4.1	47	5.4	0.39406	6	4.41662	20.4	15.6	4.1	47.5	6.4	0.389306
3	4.485	100	11.6	0.0224231	8	0.294566	1.4	1	4.485	99.1	8.4	0.019767
4	4.717	132	15.3	0.176141	4	1.38996	6.4	4.9	4.717	131.9	4.4	0.176864
5	5.066	180	20.9	0.0844941	3	0.414417	1.9	1.5	5.066	180.1	2.8	0.0843663
6	5.167	194	22.5	0.05168	5	0.407945	1.9	1.4	5.167	194.4	4.7	0.0494706
7	5.241	206	23.9	0.0296194	6	0.29844	1.4	1.1	5.241	204.9	6	0.0280383
8	5.518	256	29.7	0.0454648	5	0.365797	1.7	1.3	5.518	256.4	4.6	0.0448083
9	5.629	276	32	0.361011	4	2.64198	12.2	9.3	5.629	275.9	4.2	0.351521
10	5.695	288	33.4	0.324143	5	2.98537	13.8	10.5	5.695	287.3	5.3	0.315926
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.75	298	34.5	0.162385	5	1.27995	5.9	4.5	5.75	298.6	4.5	0.160049
12	5.909	328	38	0.0837041	4	0.556593	2.6	2	5.909	327.7	3.8	0.0828656
13	5.958	338	39.2	0.0183305	3	0.0896314	0.4	0.3	5.958	338.1	2.7	0.0184433
14	6.007	348	40.3	0.0262675	5	0.220903	1	0.8	6.007	348.5	5.1	0.0242862
15	6.061	359	41.6	0.0846356	3	0.500304	2.3	1.8	6.061	358.2	3.3	0.0848194
16	6.105	368	42.6	0.0162706	4	0.116869	0.5	0.4	6.105	368.9	4	0.0164109
17	6.233	394	45.7	0.112472	3	0.623784	2.9	2.2	6.233	394.4	3.2	0.110974
18	6.341	416	48.2	0.0333252	3	0.181588	0.8	0.6	6.341	415.6	3.4	0.0303653
19	6.39	426	49.4	0.00923769	4	0.0708745	0.3	0.3	6.39	427.9	4	0.00987698
20	6.474	443	51.3	0.181546	2	0.734286	3.4	2.6	6.474	443	2.3	0.178396
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.65	469	54.3	0.0128601	5	0.0849018	0.4	0.3	6.65	468.5	4.6	0.0103967
22	6.88	494	57.2	0.0352987	3	0.171275	0.8	0.6	6.88	494.2	2.7	0.0357729
23	6.947	503	58.3	0.117234	2	0.498375	2.3	1.8	6.947	502.7	2.3	0.121649

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	110	12.7	0.0924389	4	0.671235	6.5	4.6	4.55	110.4	4.3	0.0882957
2	5.2	200	23.1	0.0886279	5	0.728999	7.1	5	5.2	200.4	4.7	0.0867029
3	5.85	317	36.7	0.18206	4	1.30232	12.6	8.9	5.85	316.5	4	0.184377
4	6.55	455	52.7	0.126121	8	1.63974	15.9	11.2	6.55	454	8	0.116023
5	6.85	488	56.5	0.0519618	5	0.401688	3.9	2.7	6.85	489.1	4.6	0.0497548
6	7.35	558	64.6	0.0918281	5	0.760434	7.4	5.2	7.35	557.5	4.8	0.0886053
7	8.15	681	78.8	0.100704	9	1.4801	14.3	10.1	8.15	681.2	8.7	0.095598
8	8.45	748	86.6	0.135268	8	1.81471	17.6	12.4	8.45	747.8	7.7	0.132113
9	8.65	798	92.4	0.095261	5	0.841619	8.2	5.8	8.65	797.2	5	0.0940988
10	9.3	846	97.9	0.05703	8	0.673746	6.5	4.6	9.3	843.1	8	0.0477964

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.893	24	2.8	0.204654	7	2.72982	11.6	9.4	3.893	24.6	7.3	0.210846
2	4.066	48	5.5	0.335659	6	3.55526	15.1	12.2	4.066	48.4	6	0.332557
3	4.434	99	11.4	0.0179307	6	0.186503	0.8	0.6	4.434	101	6.1	0.0172536
4	4.55	115	13.2	0.0523646	4	0.340674	1.4	1.2	4.55	115.5	3.8	0.0510362
5	4.687	134	15.4	0.139341	4	1.03617	4.4	3.6	4.687	134.4	4.1	0.141045
6	5.027	181	20.9	0.0679562	3	0.326559	1.4	1.1	5.027	181.1	2.8	0.066591
7	5.128	195	22.5	0.0327283	5	0.27617	1.2	0.9	5.128	195.2	4.8	0.0324777
8	5.2	205	23.6	0.0560919	4	0.408999	1.7	1.4	5.2	204	4.1	0.0563913
9	5.48	255	29.4	0.0616577	9	0.863096	3.7	3	5.48	256.6	8.7	0.0559349
10	5.592	275	31.7	0.323351	5	2.65728	11.3	9.1	5.592	274.4	4.7	0.317316
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.659	287	33.1	0.280351	6	3.18611	13.5	10.9	5.659	286.9	6.3	0.284326
12	5.732	300	34.6	0.115789	6	1.24249	5.3	4.3	5.732	298.9	6	0.116978
13	5.85	321	37	0.0577113	4	0.441918	1.9	1.5	5.85	321.7	4.2	0.0591618
14	5.882	327	37.7	0.0637376	3	0.348476	1.5	1.2	5.882	327.8	3.5	0.0565918
15	5.958	341	39.3	0.0202625	3	0.0915033	0.4	0.3	5.958	340.9	2.6	0.0201835
16	6.012	351	40.4	0.0290066	4	0.18418	0.8	0.6	6.012	351.2	4	0.0262361
17	6.065	361	41.6	0.0781071	2	0.314494	1.3	1.1	6.065	360.5	2.2	0.0794678
18	6.119	371	42.7	0.0105188	3	0.0476032	0.2	0.2	6.119	371.2	2.6	0.010278
19	6.243	394	45.4	0.0893626	3	0.465721	2	1.6	6.243	393.9	3	0.0883894
20	6.302	405	46.7	0.0172042	6	0.136774	0.6	0.5	6.302	405.1	5.6	0.0138838
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.345	413	47.6	0.0240017	2	0.0993934	0.4	0.3	6.345	413.5	2.4	0.0233254
22	6.399	423	48.7	0.0133846	11	0.337782	1.4	1.2	6.399	431.2	11.1	0.0172345
23	6.485	439	50.6	0.164129	2	0.560186	2.4	1.9	6.485	438.8	2.3	0.134848
24	6.55	451	52	0.0252318	9	0.481135	2	1.7	6.55	446.3	9.3	0.0292389
25	6.662	465	53.6	0.0105254	3	0.0302599	0.1	0.1	6.662	465.4	2.7	0.00639034
26	6.867	491	56.6	0.0461207	6	0.400048	1.7	1.4	6.867	489.5	5.5	0.0406833
27	6.934	500	57.6	0.108062	2	0.44425	1.9	1.5	6.934	499.9	2.3	0.10948
28	7.35	556	64.1	0.0565442	7	0.6556	2.8	2.2	7.35	555.7	7	0.0526656
29	8.15	682	78.6	0.031997	8	0.413353	1.8	1.4	8.15	682.1	7.8	0.029789
30	8.45	754	86.9	0.0626534	6	0.650737	2.8	2.2	8.45	753.8	6.2	0.0588567
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.65	802	92.4	0.0460133	5	0.353197	1.5	1.2	8.65	801.8	4.7	0.0426248
32	9.3	847	97.6	0.0386216	6	0.328327	1.4	1.1	9.3	845.9	5.6	0.0331138

PeakCounter: 32

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.875	18	2.1	0.288967	7	3.75854	16	11.8	3.875	19	7.3	0.289644
2	4.046	42	4.9	0.406703	6	4.11595	17.6	12.9	4.046	42.9	5.8	0.40308
3	4.429	96	11.1	0.0248712	7	0.294278	1.3	0.9	4.429	95.2	7.4	0.022445
4	4.671	130	15	0.187423	4	1.41901	6.1	4.4	4.671	130.1	4.2	0.188444
5	5.012	178	20.6	0.0974093	3	0.470888	2	1.5	5.012	177.7	2.8	0.0963676
6	5.083	188	21.7	0.0882249	4	0.625204	2.7	2	5.083	188.2	4.5	0.079135
7	5.154	198	22.9	0.0538503	6	0.557068	2.4	1.7	5.154	197.2	5.8	0.0538863
8	5.45	248	28.7	0.0543944	5	0.505178	2.2	1.6	5.45	249.1	5.4	0.052733
9	5.577	270	31.2	0.42575	4	2.64158	11.3	8.3	5.577	270.4	3.6	0.412256
10	5.657	284	32.8	0.363977	5	3.09031	13.2	9.7	5.657	283.2	5.1	0.344904
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.721	295	34.1	0.142622	6	1.47857	6.3	4.6	5.721	294.8	5.8	0.14294
12	5.858	319	36.9	0.0777709	4	0.533977	2.3	1.7	5.858	318.6	3.7	0.0807292
13	5.919	331	38.3	0.0284447	2	0.120171	0.5	0.4	5.919	331.4	2.4	0.0286932
14	5.976	342	39.5	0.0349883	4	0.269078	1.1	0.8	5.976	341.6	4.3	0.0351173
15	6.022	351	40.6	0.0879717	3	0.530181	2.3	1.7	6.022	350.9	3.3	0.0906283
16	6.079	362	41.8	0.0102122	3	0.0533663	0.2	0.2	6.079	362.3	2.9	0.0104081
17	6.203	386	44.6	0.128806	3	0.6719	2.9	2.1	6.203	386.6	3	0.126222
18	6.275	400	46.2	0.0185177	9	0.260136	1.1	0.8	6.275	397.8	8.7	0.0168485
19	6.311	407	47.1	0.0426809	2	0.161152	0.7	0.5	6.311	407.7	2.4	0.0385796
20	6.372	419	48.4	0.0129065	4	0.0741023	0.3	0.2	6.372	419.7	3.7	0.0112214
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.465	437	50.5	0.173318	3	0.850031	3.6	2.7	6.465	436.7	2.8	0.17424
22	6.643	464	53.6	0.0166575	5	0.124329	0.5	0.4	6.643	463.8	4.9	0.0142246
23	6.884	492	56.9	0.0430969	3	0.223072	1	0.7	6.884	491.7	3	0.0415003
24	6.944	500	57.8	0.132907	2	0.606985	2.6	1.9	6.944	500.5	2.5	0.137518

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	109	12.6	0.0700553	4	0.524441	4.8	3.7	4.55	109.2	4.5	0.0659622
2	5.2	202	23.3	0.0930642	4	0.658584	6	4.6	5.2	202.1	4	0.0919027
3	5.85	312	36	0.186863	4	1.33823	12.2	9.4	5.85	312.3	4	0.190505
4	6.55	454	52.4	0.16767	6	1.68888	15.4	11.8	6.55	453.9	6.1	0.157237
5	6.85	484	55.9	0.0719322	5	0.58616	5.3	4.1	6.85	484.1	4.7	0.0700771
6	7.35	552	63.7	0.11216	5	0.910485	8.3	6.4	7.35	551.8	4.7	0.109503
7	8.15	678	78.3	0.0949027	8	1.29188	11.7	9	8.15	677.7	7.9	0.092241
8	8.45	744	85.9	0.139204	7	1.67943	15.3	11.7	8.45	743	6.9	0.138083
9	8.65	797	92	0.106662	5	0.917035	8.3	6.4	8.65	796.7	5.1	0.100628
10	9.3	845	97.6	0.106805	10	1.4057	12.8	9.8	9.3	841.7	9.7	0.0815356

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 109

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 6.900 5.200 8.700 5.850 13.900 6.550 17.100 6.850 4.900 7.350 7.600 8.150 14.000 8.450 18.100 8.650 8.400 9.300 0.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
3.925 15.200 4.100 20.400 4.485 1.400 4.717 6.400 5.066 1.900 5.167 1.900 5.241 1.400 5.518 1.700 5.629 12.200 5.695 13.800 5.750 5.900 5.909 2.600 5.958 0.400 6.007 1.000 6.061 2.300 6.105 0.500 6.233 2.900 6.341 0.800 6.390 0.300 6.474 3.400 6.650 0.400 6.880 0.800 6.947 2.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 23 Return to Lane Navigator
4.550 6.500 5.200 7.100 5.850 12.600 6.550 15.900 6.850 3.900 7.350 7.400 8.150 14.300 8.450 17.600 8.650 8.200 9.300 6.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
3.893 11.600 4.066 15.100 4.434 0.800 4.550 1.400 4.687 4.400 5.027 1.400 5.128 1.200 5.200 1.700 5.480 3.700 5.592 11.300 5.659 13.500 5.732 5.300 5.850 1.900 5.882 1.500 5.958 0.400 6.012 0.800 6.065 1.300 6.119 0.200 6.243 2.000 6.302 0.600 6.345 0.400 6.399 1.400 6.485 2.400 6.550 2.000 6.662 0.100 6.867 1.700 6.934 1.900 7.350 2.800 8.150 1.800 8.450 2.800 8.650 1.500 9.300 1.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 32 Return to Lane Navigator
3.875 16.000 4.046 17.600 4.429 1.300 4.671 6.100 5.012 2.000 5.083 2.700 5.154 2.400 5.450 2.200 5.577 11.300 5.657 13.200 5.721 6.300 5.858 2.300 5.919 0.500 5.976 1.100 6.022 2.300 6.079 0.200 6.203 2.900 6.275 1.100 6.311 0.700 6.372 0.300 6.465 3.600 6.643 0.500 6.884 1.000 6.944 2.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 24 Return to Lane Navigator
4.550 4.800 5.200 6.000 5.850 12.200 6.550 15.400 6.850 5.300 7.350 8.300 8.150 11.700 8.450 15.300 8.650 8.300 9.300 12.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Blue Catfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Blue Catfish ---- Go to Display Gel Lane Navigator.