Projects

IMPROVEMENT OF STEM CELL AND NUCLEAR CLONING TECHNOLOGIES IN UNGULATES Appropriated (D)   Accession Number: 412304

FUNCTIONAL GENOMICS OF ENHANCED EMBRYO, FETAL, AND NEONATAL DEVELOPMENT AND SURVIVAL IN SWINE Appropriated (D)   Accession Number: 412099

Publications

Critical control points in the impact of proinflammatory immune response on growth and metabolism - (Peer Reviewed Journal)

Elsasser, T.H., Caperna, T.J., Li, C.J., Kahl, S., Sartin, J.L. 2008. Critical control points in the impact of the proinflammatory immune response on growth and metabolism. Journal of Animal Science. 86(E. Suppl):E105-E 125.

Ontogeny of adipokine expression in neonatal pig adipose tissue - (Peer Reviewed Journal) - (29-Sep-08)

Natural Variation of Storage Proteins and Isoflavones in Wild and Cultivated Soybeans - (Abstract)

Natarajan, S.S., Luthria, D.L., Bae, H., Caperna, T.J., Garrett, W.M., Cregan, P.B., Song, Q., Xu, C. 2007. Natural variation of storage proteins and isoflavones in wild and cultivated soybeans. Biosafety Workshop Proceedings. p. 37-39.

A gel-based reference map of the porcine hepatocyte proteome - (Peer Reviewed Journal)

Caperna, T.J., Shannon, A., Garrett, W.M. 2008. A gel-based reference map of the porcine hepatocyte proteome. Domestic Animal Endocrinology. 35(1):142-156.

Utility of proteomics techniques for assessing protein expression - (Proceedings/Symposium)

Natarajan, S.S., Xu, C., Cregan, P.B., Caperna, T.J., Garrett, W.M., Luthria, D.L. 2007. Utility of proteomics techniques for assessing protein expression. Meeting Abstract. p. 51-55.

Application of proteomic tools for assessing transgenic soybeans for biosafety studies - (Proceedings/Symposium)

Natarajan, S.S., Xu, C., Caperna, T.J., Garrett, W.M., Cregan, P.B., Luthria, D.L. 2007. Application of proteomic tools for assessing transgenic soybeans for biosafety studies. Brazilian Biosafety Conference. p. 129.

Determination of optimal protein quantity required to identify abundant and less abundant soybean seed proteins by 2D-PAGE and MS - (Peer Reviewed Journal)

Natarajan, S.S., Xu, C., Bae, H., Caperna, T.J., Garrett, W.M. 2007. Determination of optimal protein quantity required to identify abundant and less abundant soybean seed proteins by 2D-PAGE and MS. Plant Molecular Biology Reporter. 25:55-62.

Nitration of JAK-2 at the 1007Y-1008Y activation epitope impedes phosphorylation at this site: defining a GH, AKT/protein kinase B and nitric oxide synthase axis - (Peer Reviewed Journal)

Elsasser, T.H., Li, C., Caperna, T.J., Kahl, S., Schmidt, W.F. 2007. GH-associated nitration of JAK2 at the 1007Y-1008Y epitope impedes phosphorylation at this site: Mechanism for and impact of a GH, AKT and nitric oxide synthase (NOS) axis on GH signal transduction. Endocrinology. 148:3792-3802. Available: http://endo.endojournals.org.

Impact of Solar Ultraviolet-B Radiation on the Proteome in Soybean Lines Differing in Flavonoid Contents - (Peer Reviewed Journal)

Xu, C., Sullivan, J.H., Garrett, W.M., Caperna, T.J., Natarajan, S.S. 2007. Impact of Solar Ultraviolet-B Radiation on the Proteome in Soybean Lines Differing in Flavonoid Contents. Proteomics. 69:38-48.

Proteomic analysis of the distribution of the major seed allergens in sixteen soybean genotypes - (Peer Reviewed Journal)

Xu, C., Caperna, T.J., Garrett, W.M., Cregan, P.B., Bae, H., Luthria, D.L., Natarajan, S.S. 2007. Proteomic analysis of the distribution of the major seed allergens in sixteen soybean genotypes. Journal of the Science of Food and Agriculture. 87:2511-2518.

Proteomic and genetic analysis of glycinin subunits of sixteen soybean genotypes - (Peer Reviewed Journal)

Natarajan, S.S., Xu, C., Bae, H., Bailey, B.A., Cregan, P.B., Caperna, T.J., Garrett, W.M., Luthria, D.L. 2007. Proteomic and genetic analysis of glycinin subunits of sixteen soybean genotypes. Plant Physiology and Biochemistry. 45(6):436-444.

Are There Any Correlations Between Proteins and Bioactive Isoflavones in Soybeans? - (Abstract)

Xu, C., Luthria, D.L., Bae, H., Caperna, T.J., Garrett, W.M., Song, Q., Cregan, P.B., Natarajan, S.S. 2007. Are there any correlations between proteins and bioactive isoflavones in soybeans?.[abstract]. BARC Poster Day.

Comparison of the Interferon-tau Expression from Primary Trophectoderm Outgrowths Derived from IVP, NT, and Parthenogenote Bovine Blastocysts - (Peer Reviewed Journal)

Talbot, N.C., Powell, A.M., Ocon, O.M., Caperna, T.J., Camp, M.J., Garrett, W.M., Ealy, A.D. 2008. Comparison of the Interferon-tau Expression from Primary Trophectoderm Outgrowths Derived from IVP, NT, and Parthenogenote Bovine Blastocysts. Journal of Molecular Reproduction and Development. 75(2):299-308.

Isolation and Characterization of Porcine Visceral Endoderm Cell Lines Derived from in Vivo 11-Day Blastocysts - (Peer Reviewed Journal)

Talbot, N.C., Blomberg, L., Mahmood, A., Caperna, T.J., Garrett, W.M. 2007. Isolation and characterization of porcine visceral endoderm cell lines derived from in vivo 11-day blastocysts. In Vitro Cellular and Developmental Biology - Animals. 43(2):72-86.

Characterization of Major Allergen Proteins of Soybean by Proteomic Tools - (Abstract)

Natarajan, S.S., Xu, C., Caperna, T.J., Cregan, P.B., Bae, H., Garrett, W.M. 2006. Characterization of major allergen proteins of soybean by proteomic tools. Mid Atlantic Plant Molecular Biology Society Conference 2006. August 17-18, 2006. Beltsville MD. Abstract #11. .

Proteomic Analyses of Male Contributions to Honey Bee Sperm Storage and Mating. - (Peer Reviewed Journal)

Collins, A.M., Caperna, T.J., Williams, V.P., Garrett, W.M., Evans, J.D. 2006.Proteomic analyses of male contributions to honey bee sperm storage and mating. Insect Molecular Biology. 15(5):541-549.

Proteomic Analysis of Allergen and Antinutritional Proteins in Wild and Cultivated Soybean - (Peer Reviewed Journal)

Natarajan, S.S., Xu, C., Bae, H., Caperna, T.J., Garrett, W.M. 2006. Proteomic analysis of allergen and antinutritional proteins in wild and cultivated soybean. Journal of Plant Biochemistry and Biotechnology. 15: 103-108.

Separation and Identification of Soybean Leaf Proteins by Two-Dimensional Gel Electrophoresis and Mass Spectrometry - (Peer Reviewed Journal)

Xu, C., Garrett, W.M., Sullivas, J., Caperna, T.J., Natarajan, S.S. 2006. Separation and identification of soybean leaf proteins by two-dimensional gel electrophoresis and mass spectrometry. Phytochemistry. 67: 2431-2440.

Identification and Characterization of Aquaporin-9 (Aqp9) in Porcine Hepatic Tissue and Hepatocytes in Monolayer Culture - (Peer Reviewed Journal)

Caperna, T.J., Shannon, A.E., Richards, M.P., Garrett, W.M., Talbot, N.C. 2006. Identification and characterization of aquaporin-9 (aqp9) in porcine hepatic tissue and hepatocytes in monolayer culture. Domestic Animal Endocrinology. 32:273-286.

Growth Hormone (Gh) Directs Nitration of the Tyrosine Phosphorylation Site of the Jak-2 (1007-1008y) in Hepatocytes Through An Akt/phosphorylase Kinase B Activation of Endothelial Nitric Oxide Synthase (Enos) - (Abstract)

Elsasser, T.H., Caperna, R.J., Li, C., Kahl, S. 2006. Growth hormone (GH) directs nitration of the tyrosine phosphorylation site of JAK-2 (1007-1008Y) in hepatocytes through an AKT/Phosphorylase Kinase B activation of endothelial nitric oxide synthase (eNOS) [abstract]. Proceedings of the Annual Meeting of the Endocrine Society. p. 44.

Characterization of Storage Proteins in Wild (Glycine Boja) and Cultivated (Glycine Max) Soybean Seeds Using Proteomic Analysis. - (Peer Reviewed Journal)

Natarajan, S.S., Xu, C., Bae, H., Caperna, T.J., Garrett, W.M. 2006. Characterization of storage proteins in wild (glycine boja) and cultivated (glycine max) soybean seeds using proteomic analysis.. Journal of Agricultural and Food Chemistry. v. 54:3114-3120.

The Igf System in Chickens: Factors Affecting Gene Expression in Muscle and Liver - (Abstract)

Kuo, A., Clover, C.M., Rosebrough, R.W., McMurtry, J.P., Mitchell, A.D., Caperna, T.J., Li, C., Kahl, S., Elsasser, T.H. 2006. The IGF system in chickens: Factors affecting gene expression in muscle and liver [abstract]. Journal of Federation of American Societies for Experimental Biology. 20:A172.

Proteomic Characterization of Allergen Proteins in Sixteen Soybean Genotypes - (Abstract)

Natarajan, S.S., Xu, C., Caperna, T.J., Cregan, P.B., Bae, H., Garrett, W.M. 2006. Proteomic characterization of allergen proteins in sixteen soybean genotypes [abstract]. Plant Biology Annual Meeting 2006. August 05-09, Boston, MA. Abstract No. 32025.

Adrenomedullin (Am) and Adrenomedullin Binding Protein (Am-Bp) in the Bovine Mammary Gland and Milk: Effects of Stage of Lactation and Experimental Intramammary Coliform Infection - (Peer Reviewed Journal)

Elsasser, T.H., Capuco, A.V., Caperna, T.J., Martinez, A., Cuttitta, F., Kahl, S. 2006. Adrenomedullin (AM) and adrenomedullin binding protein (AM-BP) in the bovine mammary gland and milk: Effects of stage of lactation and experimental intramammary E. coli infection. Domestic Animal Endocrinology. Available:http://dx.doi.org/doi:10.1016/j.domaniend. 32:138-154.

Separation and Identification of Soybean Leaf Proteins by 2d-Page and Mass Spectrometry - (Abstract)

Xu, C., Garrett, W.M., Sullivan, J., Caperna, T.J., Natarajan, S.S. 2005. Separation and identification of soybean leaf proteins by 2d-page and mass spectrometry [abstract]. Mid Atlantic Plant Molecular Biology Society Conference. Paper No. 16.

Proteomic Analysis of Beta-Gonglycinin and Glycinin in Different Soybean Genotypes - (Abstract)

Natarajan, S.S., Cregan, P.B., Xu, C., Caperna, T.J., Garrett, W.M., Sullivan, J. 2005. Proteomic analysis of beta-gonglycinin and glycinin in different soybean genotypes [abstract]. Mid Atlantic Plant Molecular Biology Society Conference. Paper No. 7.

Room Temperature Storage Effects on Picm-19h Cell Function: Artificial Liver Device Implications - (Abstract)

Mahmood, A., Hwang, D., Talbot, N.C., Caperna, T.J. 2005. Room temperature storage effects on PICM-19H cell function: artificial liver device implications. Proceedings of the Biomedical Engineering Society.

Proteomic Analysis of the Major Cellular Proteins of Bovine Trophectoderm Cell Lines Derived from Ivp, Parthenogenetic and Nuclear Transfer Embryos: Reduced Expression of Annexin I in Nuclear Transfer-Derived Cell Lines - (Abstract)

Talbot, N.C., Powell, A.M., Caperna, T.J., Garrett, W.M. 2005. Proteomic analysis of the major cellular proteins of bovine trophectoderm cell lines derived from ivp, parthenogenetic and nuclear transfer embryos: reduced expression of annexin I in nuclear transfer-derived cell lines. International Embryo Transfer Society Annual Meeting.

Tumor Necrosis Factor-a (Tnf-A) Modulate Insulin-Like Growth Factor Binding Protein (Igfbps) Activity from Cultured Epithelial Via Igfbp-3 Protease Activity - (Abstract)

Elsasser, T.H., Sartin, J.L., Li, C., Kahl, S. 2005. Tumor necrosis factor-a decreases media content of epithelial cell-derived insulin-like growth factor binding proteins (IGFBP) in part through increased proteolytic degradation of IGFBP-3 [abstract]. Journal of Animal Science. 83(Suppl.1):209.

Comparison of Protein Solubilization Method Suitable for Proteomic Analysis of Soybean Seed Proteins - (Peer Reviewed Journal)

Natarajan, S.S., Xu, C., Caperna, T.J., Garrett, W.M. 2005. Comparison of protein solubilization method suitable for proteomic analysis of soybean seed proteins. Analytical Biochemistry. 342: 214-220.

Isolation and Characterization of a Bovine Visceral Endoderm Cell Line Derived from a Parthenogenetic Blastocyst - (Peer Reviewed Journal)

Talbot, N.C., Caperna, T.J., Powell, A.M., Ealy, A.D., Blomberg, L., Garrett, W.M. 2005. Isolation and characterization of a bovine visceral endoderm cell line derived from a parthenogenetic blastocyst. Vitro Cellular and Developmental Biology. 41:130-141.

Hormonal Regulation of Leptin Receptor Expression in Primary Cultures of Porcine Hepatocytes - (Peer Reviewed Journal)

Caperna, T.J., Shannon, A.E., Poch, S.M., Garrett, W.M., Richards, M.P. 2005. Hormonal regulation of leptin receptor expression in primary cultures of porcine hepatocytes. Domestic Animal Endocrinology. 29:582-592.

Hormonal Regulation of Leptin Receptor Expression in Primary Cultures of Porcine Hepatocytes - (Abstract)

Caperna, T.J., Shannon, A.E., Poch, S.M., Garrett, W.M., Richards, M.P. 2004. Hormonal regulation of leptin receptor expression in primary cultures of porcine hepatocytes [abstract]. Journal of Animal Science. 82(Suppl.1):200.

Proteomic Analysis of Turkey Sperm Storage Tubules - (Abstract)

McDougal, J.G., Garrett, W.M., Long, J.A., Caperna, T.J., Zuelke, K.A. Proteomic analysis of turkey sperm storage tubules. BARC Poster Day. 2004. Abstract No.

Isolation and Characterization of a Bovine Trophectoderm Cell Line derived from a Parthenogenetic Blastocyst. - (Peer Reviewed Journal)

Talbot, N.C., Caperna, T.J., Powell, A.M., Garrett, W.M., Ealy, A.D. 2004. Isolation and characterization of a bovine trophectoderm cell line derived from a parthenogenetic blastocyst. Molecular Reproduction and Development. 69(2):164-173.

Proteome Analysis of Soybean Proteins - (Abstract)

Natarajan, S.S., Xu, C., Caperna, T.J., Garrett, W.M. 2004. Proteome analysis of soybean proteins [abstract]. BARC Poster Day. Abstract p.17.

Selection of An Efficient Method to Characterize Soybean Seed Proteins for 2d and Maldi-Tof Analysis - (Abstract)

Natarajan, S.S., Xu, C., Caperna, T.J., Garrett, W.M. 2004. Selection of an efficient method to characterize soybean seed proteins for 2D and MALDI-TOF analysis [abstract]. American Society of Plant Biologists Annual Meeting. Abstract 710.

Plasma Clearance and Tissue Distribution of Radiolabeled Leptin in the Chicken - (Peer Reviewed Journal)

McMurtry, J.P., Ashwell, C.M., Brocht, D.M., Caperna, T.J. 2004. Plasma clearance and tissue distribution of radiolabeled leptin in the chicken. Comparative Biochemistry and Physiology. Part A 138:27-32.

The Role of Insulin, Glucagon, Dexamethasone and Leptin in the Regulation of Ketogenesis and Glycogen Storage in Primary Cultures of Porcine Hepatocytes Prepared from Growing Pigs - (Peer Reviewed Journal)

Fernandez-Figares, I., Shannon, A.E., Wray-Cahen, D., Caperna, T.J. 2004. The role of insulin glucagons, dexamethasone and leptin in the regulation ketogenesis and glycogen storage in primary cultures of porcine hepatocytes prepared from 60 kg pigs. Domestic Animal Endocrinology. 27(2):125-140.

Colony-Formation Efficiency of Bovine Fetal Fibroblast Cell Lines Cultured with Low Oxygen, Hydrocortisome, L-Carnosine, Or Bfgf. - (Peer Reviewed Journal)

Talbot, N.C., Powell, A.M., Caperna, T.J. 2004. Colony-formation efficiency of bovine fetal fibroblast cell lines cultured with low oxygen, hydrocortisone, L-carnosine, or BFGF. Cloning and Stem Cells. 6(1):35-45.

Betaine Improves Growth But Does Not Induce Whole Body Or Hepatic Palmitate Oxidation in Swine (Sus Scrofa Dometica) - (Peer Reviewed Journal)

Wray-Cahen, D., Fernandez-Figares, I., Virtanen, E., Steele, N.C., Caperna, T.J. 2004. Betaine improves growth but does not induce whole body or hepatic palmitate oxidation in swine (sus scrofa domestica). Comparative Biochemistry and Physiology. Jan;137(1):131-40.