Projects

GENOMIC AND IMMUNOLOGICAL CHARACTERISTICS OF JOHNE'S DISEASE Appropriated (D)   Accession Number: 411740

EFFECT OF FEEDING HEAT-TREATED COLOSTRUM MYCOBACTERIUM PARATUBERCULOSIS (MAP) IN DAIRY CALVES: PHASE 1/06-01731 Reimbursable (R)   Accession Number: 413151

JOHNE'S DISEASE INTEGRATED PROGRAM IN RESEARCH, EDUCATION, AND EXTENSION Reimbursable (R)   Accession Number: 408473

PREVENTION AND TREATMENT OF MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS (MAP) INFECTION IN MICE BY LACTOBACILLUS ACIDOPHILUS NP51 Reimbursable (R)   Accession Number: 413281

Publications

Biomarker Discovery in Subclinical Mycobacterial Infections of Cattle - (Peer Reviewed Journal) - (09-Apr-09)

Role of Nitric Oxide Production in Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis - (Peer Reviewed Journal) - (27-Mar-09)

Evaluation of Control Points in Youngstock and Adult Dairy Cow Management to Control Transmission of Mycobacterium avium subsp. paratuberculosis - (Abstract)

Godden, S., Wells, S., Gardner, I., Fetrow, J., Stabel, J.R. 2008. Evaluation of Control Points in Youngstock and Adult Dairy Cow Management to Control Transmission of Mycobacterium avium subsp. paratuberculosis [abstract]. p.

Pathogenesis of Mycobacterium avium subsp. paratuberculosis in Neonatal Calves after Oral or Intraperitoneal Experimental Infection - (Peer Reviewed Journal)

Stabel, J.R., Palmer, M.V., Harris, B.N., Plattner, B., Hostetter, J., Robbe-Austerman, S. 2009. Pathogenesis of Mycobacterium avium subsp. paratuberculosis in Neonatal Calves after Oral or Intraperitoneal Experimental Infection. Veterinary Microbiology. 136(3-4):306-313.

Differential Expression of CD5 on B Lymphocytes in Cattle Infected with Mycobacterium avium subsp. paratuberculosis - (Peer Reviewed Journal)

Stabel, J.R., Khalifeh, M.S. 2008. Differential Expression of CD5 on B Lymphocytes in Cattle Infected with Mycobacterium avium subsp. paratuberculosis. Veterinary Immunology and Immunopathology. 126(3-4):211-219.

Osteopontin Immunoreactivity in the Ileum and Ileoceccal Lymph Node of Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis - (Peer Reviewed Journal)

Karcher, E.L., Johnson, C.S., Beitz, D.C., Stabel, J.R. 2008. Osteopontin Immunoreactivity in the Ileum and Ileoceccal Lymph Node of Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis. Veterinary Immunology and Immunopathology. 126(1-2):142-148.

Effect of Feeding Raw Versus Heat-treated Colostrum on Passive Transfer of Immunoglobulin G in Newborn Dairy Calves - (Abstract)

Donahue, M., Godden, S., Bey, R., Wells, S., Fetrow, J., Stabel, J.R. 2008. Effect of Feeding Raw Versus Heat-treated Colostrum on Passive Transfer of Immunoglobulin G in Newborn Dairy Calves [abstract]. The American Association of Bovine Practitioners.

Osteopontin: A Novel Cytokine Involved in the Regulation of Mycobacterium avium subsp. paratuberculosis Infection in Periparturient Dairy Cattle - (Peer Reviewed Journal)

Karcher, E.L., Bayles, D.O., Bannantine, J.P., Beitz, D.C., Stabel, J.R. 2008. Osteopontin: A Novel Cytokine Involved in the Regulation of Mycobacterium avium subsp. paratuberculosis Infection in Periparturient Dairy Cattle. Journal of Dairy Science. 91(8):3079-3091.

Immune Response to Orally Administered Killed MAP in Calves - (Abstract)

Sweeney, R.W., Stabel, J.R., Whitlock, R.H. 2008. Immune Response to Orally Administered Killed MAP in Calves [abstract]. Johne's Disease Integrated Program.

Modulation of Cytokine Gene Expression and Secretion During the Periparturient Period in Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis - (Peer Reviewed Journal)

Karcher, E.L., Beitz, D.C., Stabel, J.R. 2008. Modulation of Cytokine Gene Expression and Secretion During the Periparturient Period in Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis. Veterinary Immunology and Immunopathology. 123(3-4):277-288.

Osteopontin Expression in Periparturient Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis - (Review Article)

Karcher, E.L., Beitz, D.C., Stabel, J.R. 2008. Osteopontin Expression in Periparturient Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis. Iowa State Animal Industry Report 2008. A.S. Leaflet No. R-2305.

Parturition invokes Changes in Peripheral Blood Mononuclear Cell Populations in Holstein Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis - (Review Article)

Karcher, E.L., Beitz, D.C., Stabel, J.R. 2008. Parturition invokes Changes in Peripheral Blood Mononuclear Cell Populations in Holstein Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis. Iowa State Animal Industry Report 2008. A.S. Leaflet No. R-2306.

Early Antibody Response Against Mycobacterium avium subspecies paratuberculosis Antigens in Subclinical Cattle - (Peer Reviewed Journal)

Bannantine, J.P., Bayles, D.O., Waters, W.R., Palmer, M.V., Stabel, J.R., Paustian, M. 2008. Early Antibody Response Against Mycobacterium avium subspecies paratuberculosis Antigens in Subclinical Cattle. Proteome Science. 6:5.

Early Immune Markets Associated with Experimental Mycobacterium avium subsp. paratuberculosis (MAP) Infection in a Neonatal Calf Model - (Abstract)

Stabel, J.R., Robbe-Austerman, S., Davis, W.C. 2008. Early Immune Markets Associated with Experimental Mycobacterium avium subsp. paratuberculosis (MAP) Infection in a Neonatal Calf Model [abstract]. Experimental Biology 2008.

Cytokine Secretion in Periparturient dairy cows naturally infected with Mycobacterium avium subsp. paratuberculosis - (Abstract)

Karcher, E.L., Beitz, D.C., Stabel, J.R. 2008. Cytokine Secretion in Periparturient dairy cows naturally infected with Mycobacterium avium subsp. paratuberculosis [abstract]. American Dairy Science Association.

Osteopontin Immunoreactivity in Peripheral Blood Mononuclear Cells, Ileum, and Ileocecal Lymph Node of Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis - (Abstract)

Karcher, E.L., Johnson, C.S., Bannantine, J.P., Beitz, D.C., Stabel, J.R. 2008. Osteopontin Immunoreactivity in Peripheral Blood Mononuclear Cells, Ileum, and Ileocecal Lymph Node of Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis [abstract]. American Dairy Science Association.

Activation of immune cells in bovine mammary gland secretions by zymosan treated bovine serum - (Peer Reviewed Journal)

Kimura, K., Goff, J.P., Schmerr, M.J., Stabel, J.R., Inumaru, S., Yokomizo, Y. 2008. Activation of immune cells in bovine mammary gland secretions by zymosan-treated bovine serum. Journal of Dairy Science. 91(5):1852-1864.

Parturition Invokes Changes in Peripheral blood Mononuclear Cell Populations in Holstein Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis - (Peer Reviewed Journal)

Karcher, E.L., Beitz, D.C., Stabel, J.R. 2008. Parturition Invokes Changes in Peripheral blood Mononuclear Cell Populations in Holstein Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis. Veterinary Immunology and Immunopathology. 124(1-2):50-62.

Parturition and Mycobacterium avium subsp. paratuberculosis: A Potential Interface for the Pathogenesis of Johne's Disease - (Abstract)

Karcher, E.L., Beitz, D.C., Stabel, J.R. 2008. Parturition and Mycobacterium avium subsp. paratuberculosis: A Potential Interface for the Pathogenesis of Johne's Disease [abstract]. American Dairy Science Association.

Pathogenesis of Mycobacterium avium subsp. paratuberculosis in Neonatal Calves after Oral or Intraperitoneal Experimental Infection - (Proceedings/Symposium)

Stabel, J.R., Palmer, M.V., Robbe-Austerman, S., Harris, B. 2008. Pathogenesis of Mycobacterium avium subsp. paratuberculosis in Neonatal Calves after Oral or Intraperitoneal Experimental Infection. In: Proceedings of the 9th International Colloquium on Paratuberculosis, October 28th-November 2, 2007, Tsukuba, Japan. p.50-53.

Immunologic Responses to Mycobacterium avium subsp. paratuberculosis in Neonatal Calves after Oral or Intraperitoneal Experimental Infection - (Proceedings/Symposium)

Stabel, J.R., Robbe-Austerman, S., Davis, W.C. 2008. Immunologic Responses to Mycobacterium avium subsp. paratuberculosis in Neonatal Calves after Oral or Intraperitoneal Experimental Infection. In: Proceedings of the 9th International Colloquium on Paratuberculosis, October 28th-November 2, 2007, Tsukuba, Japan. p.54-57.

Modulation of Cytokine Expression and Lymphocyte Subsets during the Periparturient Period in Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis - (Proceedings/Symposium)

Karcher, E.L., Beitz, D.C., Stabel, J.R. 2008. Modulation of Cytokine Expression and Lymphocyte Subsets during the Periparturient Period in Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis. In: Proceedings of the 9th International Colloquium on Paratuberculosis, October 28th-November 2, 2007, Tsukuba, Japan. p.33-36.

Profiling Host Antibody Responses to Mycobacterium avium subspecies paratuberculosis Infection Using Protein Arrays - (Peer Reviewed Journal)

Bannantine, J.P., Paustian, M., Waters, W.R., Stabel, J.R., Palmer, M.V., Li, L., Kapur, V. 2007. Profiling Host Antibody Responses to Mycobacterium avium subspecies paratuberculosis Infection Using Protein Arrays. Infection and Immunity. 76(2):739-749.

Population analysis of Fecal Microbiota from Cows Infected with Mycobacterium avium subspecies paratuberculosis - (Abstract)

Paustian, M., Fyock, T.L., Scupham, A.J., Whitlock, R.H., Stabel, J.R. 2007. Population analysis of Fecal Microbiota from Cows Infected with Mycobacterium avium subspecies paratuberculosis [abstract]. Abstract No. 112C. p. 177.

Modulation of Cytokine Expression and Lymphocyte Subsets During the Periparturient Period in Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis - (Abstract)

Karcher, E.L., Beitz, D.C., Stabel, J.R. 2007. Modulation of Cytokine Expression and Lymphocyte Subsets During the Periparturient Period in Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis [abstract]. Abstract No. 1A-01, p. 35.

Development and Use of a Partial Mycobacterium avium subspecies paratuberculosis Protein Array - (Peer Reviewed Journal)

Bannantine, J.P., Waters, W.R., Stabel, J.R., Palmer, M.V., Li, L., Kapur, V., Paustian, M. 2008. Development and Use of a Partial Mycobacterium avium subspecies paratuberculosis Protein Array. Proteomics. 8(3):463-474.

Allele, genotype, and haplotype data for BSE-resistance polymorphisms from healthy U.S. holstein cattle - (Peer Reviewed Journal)

Brunelle, B.W., Kehrli, Jr., M.E., Stabel, J.R., Moody Spurlock, D., Hansen, L.B., Nicholson, E.M. 2007. Allele, genotype, and haplotype data for bovine spongiform encephalopathy-resistance polymorphisms from healthy US holstein cattle. Journal of Dairy Science. 91:338¿342.

Immunologic Responses to Mycobacterium avium subsp. paratuberculosis in Neonatal Calves After Oral or Intraperitoneal Experimental Infection - (Abstract)

Stabel, J.R., Robbe-Austerman, S., Davis, W.C. 2007. Immunologic Responses to Mycobacterium avium subsp. paratuberculosis in Neonatal Calves After Oral or Intraperitoneal Experimental Infection [abstract]. 9th International Colloquium on Paratuberculosis. Abstract No. 29A, p. 114.

Pathogenesis of Mycobacterium avium subsp. paratuberculosis in Neonatal Calves after Oral or Intraperitoneal Experimental Infection - (Abstract)

Stabel, J.R., Palmer, M.V., Robbe-Austerman, S., Harris, B.N. 2007. Pathogenesis of Mycobacterium avium subsp. paratuberculosis in Neonatal Calves after Oral or Intraperitoneal Experimental Infection [abstract]. 9th International Colloquium on Paratuberculosis. Abstract No. 30A, p. 115.

Proposed International Guidelines for Experimental Challenge Models for Johne's Disease - (Abstract)

Sweeney, R.W., Hines Ii, M.E., Stabel, J.R. 2007. Proposed International Guidelines for Experimental Challenge Models for Johne's Disease [abstract]. 9th International Colloquium on Paratuberculosis. Abstract No. 191G-01, p. 87.

Pathology of Paratuberculosis (Johne's Disease) in Clinically Healthy Awassi Sheep with Reference to its Prevalence in Jordan - (Peer Reviewed Journal) - (23-Jul-07)

Experimental Infection of White-tailed Deer (Odocoileus virginianus) with Mycobacterium avium subsp. paratuberculosis - (Peer Reviewed Journal)

Palmer, M.V., Stabel, J.R., Waters, W.R., Bannantine, J.P., Miller, J.M. 2007. Experimental Infection of White-tailed Deer (Odocoileus virginianus) with Mycobacterium avium subsp. paratuberculosis. Journal of Wildlife Diseases. 43(4):597-608.

Pasteurization of Colostrum Reduces the Incidence of Paratuberculosis in Neonatal Calves - (Abstract)

Stabel, J.R. 2007. Pasteurization of Colostrum Reduces the Incidence of Paratuberculosis in Neonatal Calves [abstract]. American Dairy Science Association.

Osteopontin Expression During the Periparturient Period in Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis Infection - (Abstract)

Karcher, E., Beitz, D.C., Stabel, J.R. 2007. Osteopontin Expression During the Periparturient Period in Dairy Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis Infection [abstract]. American Dairy Science Association.

Experimental Challenge Models for Johne's Disease: A Review and Proposed International Guidelines - (Peer Reviewed Journal)

Hines, M.E., Stabel, J.R., Sweeney, R.W., Griffin, F., Talaat, A.M., Bakker, D., Benedictus, G., Davis, W.C., De Lisle, G.W., Gardner, I.A. 2007. Experimental Challenge Models for Johne's Disease: A Review and Proposed International Guidelines. Veterinary Microbiology. 122(2007):197-222.

Development and Characterization of Monoclonal Antibodies and Aptamers Against Major Antigens of Mycobacterium avium subsp. paratuberculosis - (Peer Reviewed Journal)

Bannantine, J.P., Radosevich, T.J., Stabel, J.R., Sreevatsan, S., Kapur, V., Paustian, M. 2007. Development and Characterization of Monoclonal Antibodies and Aptamers Against Major Antigens of Mycobacterium avium subsp. paratuberculosis. Clinical and Vaccine Immunology. 14(5):518-526.

Production and Characterization of Monoclonal Antibodies Against a Major Membrane Protein of Mycobacterium avium subsp. paratuberculosis - (Peer Reviewed Journal)

Bannantine, J.P., Radosevich, T.J., Stabel, J.R., Berger, S., Griffin, J.F., Paustian, M. 2007. Production and Characterization of Monoclonal Antibodies Against a Major Membrane Protein of Mycobacterium avium subsp. paratuberculosis. Clinical and Vaccine Immunology. 14(3):312-317.

Modulation of Cytokine Gene Expression and Secretion during the Periparturient Period in Dairy cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis - (Proceedings/Symposium)

Karcher, E.L., Beitz, D.C., Stabel, J.R. 2007. Modulation of Cytokine Gene Expression and Secretion during the Periparturient Period in Dairy cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis. Proceedings of an Iowa State University Industry Report. A.S. Leaflet #R2198.

Proteome and Differential Expression Analysis of Membrane and Cytosolic Proteins from Mycobacterium avium subsp. paratuberculosis Strains K-10 and 187. - (Peer Reviewed Journal)

Radosevich, T.J., Reinhardt, T.A., Lippolis, J.D., Bannantine, J.P., Stabel, J.R. 2007. Proteome and Differential Expression Analysis of Membrane and Cytosolic Proteins from Mycobacterium avium subsp. paratuberculosis Strains K-10 and 187. Journal of Bacteriology. 189(3):1109-1117.

Evaluation of Control Points in Youngstock and Adult Dairy Cow Management to Control Transmission of Mycobacterium avium subsp. paratuberculosis - (Abstract)

Godden, S., Wells, S., Gardner, I., Fetrow, J., Stabel, J.R. 2006. Evaluation of Control Points in Youngstock and Adult Dairy Cow Management to Control Transmission of Mycobacterium avium subsp. paratuberculosis [abstract].

HOST RESPONSES TO MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS: A COMPLEX ARSENAL - (Peer Reviewed Journal)

Stabel, J.R. 2006. Host Responses to Mycobacterium avium subsp. paratuberculosis: A Complex Arsenal. Animal Health Research Reviews. 7(1):61-70.

Skin test and Gamma Interferon enzyme-linked Immunosorbent assay results in Sheep exposed to dead Mycobacterium avium subspecies paratuberculosis Organisms - (Peer Reviewed Journal)

Robbe-Austerman, S., Stabel, J.R., Morrical, D.G. 2007. Skin test and Gamma Interferon enzyme-linked Immunosorbent assay results in Sheep exposed to dead Mycobacterium avium subspecies paratuberculosis Organisms. Journal of Veterinary Diagnostic Investigation. 19(1):88-90.

Augmentation of Secreted and Intracellular Gamma Interferon Following Johnin Purified Protein Derivative Sensitization of Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis - (Peer Reviewed Journal)

Stabel, J.R., Kimura, K., Robbe Austerman, S. 2007. Augmentation of Secreted and Intracellular Gamma Interferon Following Johnin Purified Protein Derivative Sensitization of Cows Naturally Infected with Mycobacterium avium subsp. paratuberculosis. Journal of Veterinary Diagnostic Investigation. 19:43-51.

PROTEOME AND DIFFERENTIAL EXPRESSION ANALYSIS OF MEMBRANE AND CYTOSOLIC PROTEIN FROM MYCOBACTERIUM AVIUM SUBS PARATUBERCULOSIS STRAINS K10 AND 187 - (Abstract)

Radosevich, T.J., Stabel, J.R., Bannantine, J.P., Lippolis, J.D., Reinhardt, T.A. 2006. Proteome and Differential Expression Analysis of Membrane and Cytosolic Protein from Mycobacterium avium subs paratuberculosis Strains k10 and 187 [abstract]. American Society for Microbiology. p. 112.

DEVELOPMENT AND USE OF A MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS PARTIAL PROTEIN ARRAY FOR DISCOVERY OF NOVEL ANTIGENS - (Abstract)

Bannantine, J.P., Waters, W.R., Stabel, J.R., Kapur, V., Paustian, M. 2006. Development and use of a Mycobacterium avium subsp. paratuberculosis partial protein array for discovery of novel antigens [abstract]. In: Proceedings of the American Society for Microbiology 106th Annual Meeting. May 20-25, 2006. Orlando, Florida. 2006 CDROM.

IDENTIFICATION OF DIFFERENCES IN THE PROTEIN CONTENT OF MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS PURIFIED PROTEIN DERIVATIVE PREPARATIONS WITH DIFFERENT SKIN TEST SPECIFICITIES - (Abstract)

Paustian, M., Robbe Austerman, S., Lippolis, J.D., Stabel, J.R., Bannantine, J.P. 2006. Identification of Differences in the Protein Content of Mycobacterium avium subspecies paratuberculosis Purified Protein Derivative Preparations with Different Skin Test Specificities [abstract]. American Society for Microbiology. Paper No. U-016.

PARATUBERCULOSIS: AN UPDATE - (Proceedings/Symposium)

Stabel, J.R. 2006. Paratuberculosis: an update. Proceedings of 24th World Buiatrics Congress. October 15-19, 2006, Nice, France. p. 1-8.

Heat-Treatment of Bovine Colostrum. II: Effects of Heating Duration on Pathogen Viability and Immunoglobulin G - (Peer Reviewed Journal)

Godden, S., Mcmartin, S., Feirtag, J., Stabel, J.R., Bey, R., Goyal, S., Metzger, L., Fetrow, J., Wells, S., Chester-Jones, H. 2006. Heat-Treatment of Bovine Colostrum. II: Effects of Heating Duration on Pathogen Viability and Immunoglobulin G. Journal of Dairy Science. 89(9):3476-3483.

EARLY DIAGNOSIS OF JOHNE'S DISEASE: EVALUATION OF METHODS - (Abstract)

Stabel, J.R., Robbe Austerman, S. 2006. Early diagnosis of Johne's Disease: Evaluation of Methods [abstract]. American Dairy Science Meeting. p. 133.

CHARACTERIZATION OF CYTOKINE GENE EXPRESSION IN PERIPARTURIENT DAIRY COWS NATURALLY INFECTED WITH MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS - (Abstract)

Williams, E.L., Stabel, J.R. 2006. Characterization of Cytokine gene expression in Periparturient dairy cows naturally infected with Mycobacterium avium subsp. paratuberculosis [abstract]. American Dairy Science Association Meeting. p. 110.

HEAT TREATMENT OF BOVINE COLOSTRUM. I: EFFECTS OF TEMPERATURE ON VISCOSITY AND IMMUNOGLOBULIN G LEVEL - (Peer Reviewed Journal)

Mcmartin, S., Godden, S., Metzger, L., Feirtag, J., Bey, R., Stabel, J.R., Goyal, S., Fetrow, J., Wells, S., Chester-Jones, H. 2006. Heat Treatment of Bovine Colostrum. I: Effects of Temperature on Viscosity and Immunoglobulin G Level. Journal of Dairy Science. 89(6)2110-2118

JDIP CORE 4 - ANIMAL MODELS AND FACILITIES CORE - (Abstract)

Stabel, J.R. 2006. JDIP Core 4 - Animal Models and Facilities Core [abstract]. 2nd Annual JDIP Conference. p. 20.

TIME DELAY, TEMPERATURE EFFECTS AND ASSESSMENT OF POSITIVE CONTROLS ON WHOLE BLOOD FOR THE GAMMA INTERFERON ELISA TO DETECT PARATUBERCULOSIS - (Peer Reviewed Journal)

Robbe Austerman, S., Krull, A.C., Stabel, J.R., Palmquist, D.E. 2006. Time Delay, Temperature Effects and Assessment of Positive Controls on Whole Blood for the Gamma Interferon ELISA to Detect Paratuberculosis. American Journal of Veterinary Research. 53(5):213-217.

THE MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS GENOME AND ITS INITIAL APPLICATIONS - (Abstract)

Bannantine, J.P., Kapur, V., Stabel, J.R., Paustian, M. 2005. The Mycobacterium avium subspecies paratuberculosis Genome and its Initial Applications [abstract]. AAVI-ACVM Symposium at the Conference of Research Workers in Animal Diseases. p. 16.

EVALUATION OF THE GAMMA INTERFERON ELISA IN SHEEP SUBCLINICALLY INFECTED WITH MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS USING A WHOLE CELL SONICATE OR A JOHNIN PURIFIED-PROTEIN DERIVATIVE - (Peer Reviewed Journal)

Robbe Austerman, S., Stabel, J.R., Palmer, M.V. 2006. Evaluation of the gamma interferon ELISA in sheep subclinically infected with Mycobacterium avium subspecies paratuberculosis using a whole cell sonicate or a johnin purified-protein derivative. Journal of Veterinary Diagnostic Investigation. 18(2):189-194.

A MULTI-FACTED APPROACH TO MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS RESEARCH IN THE AGRICULTURAL RESEARCH SERVICE - (Proceedings/Symposium)

Stabel, J.R., Bannantine, J.P., Paustian, M., Robbe Austerman, S. 2005. A Multi-facted Approach to Mycobacterium avium subsp. paratuberculosis Research in the Agricultural Research Service. UJNR Food & Agricultural Panel Proceedings. p. 120-123.

MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS: THE HOST RESPONSE TO A MIGHTY FOE. - (Abstract)

Stabel, J.R. 2005. Mycobacterium avium subsp. paratuberculosis: The Host Response to a Mighty Foe [abstract]. Research Workers in Animal Diseases Conference Proceedings. p. 112.

SENSITIZATION WITH JOHNIN PURIFIED PROTEIN DERIVATIVE AUGMENTS SECRETED AND INTRACELLULAR INTERFERON-GAMMA IN COWS INFECTED WITH MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS - (Proceedings/Symposium)

Stabel, J.R., Kimura, K., Robbe Austerman, S. 2006. Sensitization with johnin purified protein derivative augments secreted and intracellular interferon-gamma in cows infected with Mycobacterium avium subsp. paratuberculosis. In: Proceedings of the 8th International Colloquium on Paratuberculosis, August 13-17, 2005, Copenhagen, Denmark. p. 135-145.

DEVELOPMENT AND USE OF A MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS PARTIAL PROTEIN ARRAY FOR DISCOVERY OF NOVEL ANTIGENS - (Abstract)

Bannantine, J.P., Waters, W.R., Palmer, M.V., Kapur, V., Stabel, J.R., Paustian, M. 2006. Development and use of a mycobacterium avium subsp. paratuberculosis partial protein array for discovery of novel antigens [abstract]. International Colloquium on Paratuberculosis. p. 146.

A MULTI-FACTED APPROACH TO MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS RESEARCH IN THE AGRICULTURAL RESEARCH SERVICE - (Abstract)

Stabel, J.R., Bannantine, J.P., Paustian, M., Robbe Austerman, S. 2005. A Multi-facted Approach to Mycobacterium avium subsp. paratuberculosis Research in the Agricultural Research Service [abstract]. UJNR Food & Agricultural Panel Abstracts. p. 15.

EFFICACY OF CURRENT PASTEURIZATION STANDARDS FOR THE HEAT INACTIVATION OF MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN ULTRA-HIGH TEMPERATURE MILK - (Abstract)

Stabel, J.R., Lambertz, A. 2005. Efficacy of current Pasteurization Standards for the heat inactivation of Mycobacterium avium subsp. paratuberculosis in Ultra-high Temperature milk [abstract]. MOST, China-USDA Workshop on Agricultural Products Processing and Food Safety. p. 35.

USDA AG RESEARCH SERVICE INVESTIGATORS ARE STUDYING NEW DIAGNOSTIC TESTS, HOW THE ORGANISM WORKS, WHAT CAUSES INFECTION, AND HOW TO SLOW JOHNE'S SPREAD - (Proceedings/Symposium)

Stabel, J.R. 2006. USDA Ag Research Service Investigators are studying new diagnostic tests, how the organism works, what causes infection, and how to slow Johne's spread. Proceedings of Hoard's Dairyman. p. 7.

EXPRESSION LIBRARY IMMUNIZATION CONFERS PROTECTION AGAINST MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS INFECTION - (Peer Reviewed Journal)

Huntley, J.F., Stabel, J.R., Paustian, M., Reinhardt, T.A., Bannantine, J.P. 2005. Expression Library Immunization Confers Protection against Mycobacterium avium subsp. paratuberculosis Infection. Infection and Immunity. 73(10):6877-6884.

DEVELOPMENT OF A NESTED PCR METHOD TARGETING A UNIQUE MULTICOPY ELEMENT, ISMAP02, FOR DETECTION OF MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN FECAL SAMPLES - (Peer Reviewed Journal)

Stabel, J.R., Bannantine, J.P. 2005. Development of a Nested PCR Method Targeting a Unique Multicopy Element, ISMap02, for Detection of Mycobacterium avium subsp. paratuberculosis in Fecal Samples. Journal of Clinical Microbiology. 43(9):4744-4750.

EXPRESSION OF CD5 ON LYMPHOCYTES IN CATTLE WITH PARATUBERCULOSIS - (Abstract)

Stabel, J.R., Khalifeh, M.S., Soenksen, K. 2005. Expression of CD5 on Lymphocytes in Cattle with Paratuberculosis [abstract]. International Colloquium on Paratuberculosis. p. 161.

SENSITIZATION WITH JOHNIN PURIFIED PROTEIN DERIVATIVE AUGMENTS SECRETED AND INTRACELLULAR INTERFERON-GAMMA IN COWS INFECTED WITH MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS - (Abstract)

Stabel, J.R., Kimura, K., Robbe Austerman, S. 2005. Sensitization with Johnin Purified protein Derivative augments Secreted and Intracellular Interferon-gamma in cows infected with Mycobacterium avium subsp. paratuberculosis [abstract]. International Colloquium on Paratuberculosis. p. 135.

COMPARING THE PROBABILITY OF MAP TISSUE CULTURE POSITIVITY IN CMI NEGATIVE AND EMI POSITIVE SHEEP - (Abstract)

Robbe Austerman, S., Stabel, J.R., Palmer, M.V., Oconnor, A. 2005. Comparing the probability of map tissue culture positivity in cmi negative and emi positive sheep [abstract]. International Colloquium on Paratuberculosis. p. 570.

DETECTING CMI RESPONSES TO MYCOBACTERIUM AVIUM SSP. PARATUBERCULOSIS INFECTION IN CALVES BORN TO NATURALLY INFECTED DAMS - (Abstract)

Robbe Austerman, S., Young, S., Oconnor, A., Palmer, M.V., Stabel, J.R. 2005. Detecting CMI responses to Mycobacterium avium ssp. paratuberculosis Infection in Calves born to Naturally Infected Dams [abstract]. International Colloquium on Paratuberculosis. p. 570.

ISOLATION OF MYCOBACTERIUM AVIUM SUBSP PARATUBERCULOSIS (MAP) FROM FERAL CATS ON A DAIRY FARM WITH MAP-INFECTED CATTLE - (Peer Reviewed Journal)

Palmer, M.V., Stoffregen, W.C., Carpenter, J.G., Stabel, J.R. 2005. Isolation of Mycobacterium avium subsp paratuberculosis (MAP) from feral cats on a dairy farm with MAP-infected cattle. Journal of Wildlife Diseases. 41(3):629-635.

JOHNE'S DISEASE IN CATTLE IS ASSOCIATED WITH ENHANCED EXPRESSION OF GENES ENCODING IL-5, GATA-3, TISSUE INHIBITORS OF MATRIX METALLOPROTEINASES 1 AND 2, AND FACTORS PROMOTING APOPTOSIS IN PERIPHERAL BLOOD MONONUCLEAR CELLS - (Peer Reviewed Journal)

Coussens, P.M., Pudrith, C.B., Skovgaard, K., Xiaoning, R., Suchyta, S.P., Stabel, J.R., Heegaard, P.M. 2005. Johne's disease in cattle is associated with enhanced expression of genes encoding IL-5, GATA-3, tissue inhibitors of matrix metalloproteinases 1 and 2, and factors promoting apoptosis in peripheral blood mononuclear cells. Veterinary Immunology and Immunopathology. 105(3-4)221-234.

IMMUNOREACTIVITY OF THE MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS 19-KDA LIPOPROTEIN - (Peer Reviewed Journal)

Huntley, J., Stabel, J.R., Bannantine, J.P. 2005. Immunoreactivity of the mycobacterium avium subsp. paratuberculosis 19-kda lipoprotein. BMC Microbiology. 5(1):1-8.

DETECTION OF MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS IN FREE-RANGING BISON (BISON BISON) BY PCR - (Peer Reviewed Journal)

Ellingson, J.E., Stabel, J.R., Radcliff, R.P., Whitlock, R.H., Miller, J.M. 2005. Detection of mycobacterium avium subspecies paratuberculosis in free-ranging bison (bison bison) by PCR. Molecular and Cellular Probes. 19(3):219-225.

COMPARISON OF DIAGNOSTIC DETECTION METHODS FOR MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS FROM NORTH AMERICAN BISON - (Peer Reviewed Journal)

lHuntley, J., Whitlock, R., Bannantine, J.P., Stabel, J.R. 2005. Comparison of diagnostic detection methods for mycobacterium avium subsp. paratuberculosis from North American bison. Journal of Veterinary Diagnostic Investigation. 42(1):42-51.

PARATUBERCULOSIS AND CROHN'S DISESASE - (Proceedings/Symposium)

Stabel, J.R. 2004. Paratuberculosis and Crohn's disesase. Mastitis Council Meeting Proceedings. p. 145.

CULTURING MYCOBACTERIUM AVIUM SPP PARATUBERCULOSIS FROM SHEEP FECES IN THE UNITED STATES - (Abstract)

Robbe Austerman, S., Stabel, J.R., Harris, B.N., Johansen, K., Palmer, M.V., Payeur, J.B. 2004. Culturing mycobacterium avium spp paratuberculosis from sheep feces in the united states [abstract]. American Association of Veterinary Laboratory Diagnosticians. p. 86.

EVALUATING THE ACCURACY OF THE IFN-GAMMA ELISA AND THE SKIN TEST TO DETECT MYCOBACTERIUM AVIUM SSP. PARATUBERCULOSIS (MAP) INFECTION IN SHEEP - (Abstract)

Robbe Austerman, S., Stabel, J.R., Palmer, M.V. 2004. Evaluating the accuracy of the ifn-gamma elisa and the skin test to detect mycobacterium avium ssp. paratuberculosis (map) infection in sheep [abstract]. American Association of Veterinary Laboratory Diagnosticians. p. 87.

DEVELOPMENT AND EVALUATION OF A NEW PCR TEST FOR THE DETECTION OF MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS - (Abstract)

Stabel, J.R., Bannantine, J.P., Paustian, M. 2004. Development and Evaluation of a new PCR test for the detection of Mycobacterium avium subsp. paratuberculosis [abstract]. American Association of Veterinary Laboratory Diagnosticians.

DIFFERENTIAL EXPRESSION OF GENES ENCODING TH2 CYTOKINES, MATRIX METALLOPROTEINASES AND THEIR INHIBITORS, AND REGULATORS OF APOPTOSIS IN PBMCS OF CATTLE INFECTED WITH M. AVIUM SUBSPECIES PARATUBERCULOSIS - (Proceedings/Symposium)

Coussens, P.M., Pudrith, C.B., Ren, X., Skovgaard, K., Heegaard, P., Stabel, J.R. 2004. Differential Expression of genes encoding TH2 cytokines, Matrix Metalloproteinases and their inhibitors, and Regulators of Apoptosis in PBMCS of Cattle infected with M. avium subspecies paratuberculosis. Research Workers in Animal Diseases Conference Proceedings.

EFFICACAY OF PASTEURIZATION CONDITIONS FOR THE INACTIVATION OF MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN MILK - (Peer Reviewed Journal)

Stabel, J.R., Lambertz, A.M. 2004. Efficacy of pasteurization conditions for the inactivation of mycobacterium avium subsp. paratuberculosis in milk. Journal of Food Protection. 67(12):2719-26.

MYCOBACTERIUM PARATUBERCULOSIS: A STEALTHY KILLER - (Abstract)

Stabel, J.R. 2004. Mycobacterium paratuberculosis: A Stealthy Killer [abstract]. American College of Veterinary Internal Medicine.p. 224.

MYCOBACTERIUM PARATUBERCULOSIS: A STEALTHY KILLER - (Proceedings/Symposium)

Stabel, J.R. 2004. Mycobacterium paratuberculosis: A Stealthy Killer. Proceedings of American College of Veterinary Internal Medicine. p. 224-225.

EFFICACY OF IMMUNOLGIC ASSAYS FOR THE DETECTION OF JOHNE'S DISEASE IN DAIRY COWS FED ADDITIONAL ENERGY DURING THE PERIPARTURIENT PERIOD - (Peer Reviewed Journal)

Stabel, J.R., Goff, J.P. 2004. Efficacy of immunolgic assays for the detection of Johne's disease in dairy cows fed additional energy during the periparturient period. Journal of Veterinary Diagnostic Investigation. 16(5):412-20.

DIFFERENTIATION OF MYCOBACTERIUM BOVIS INFECTION OF CATTLE FROM M. AVIUM SUBSP. AVIUM AND M. AVIUM SUBSP. PARATUBERCULOSIS INFECTION USING A RECOMBINANT ESAT-6:CFP-10 FUSION PROTEIN - (Peer Reviewed Journal)

Waters, W.R., Nonnecke, B.J., Palmer, M.V., Robbe Austerman, S., Bannantine, J.P., Stabel, J.R., Whipple, D.L., Payeur, J.B., Estes, D.M., Pitzer, J.E., Minion, F.C. 2004. Use of recombinant esat-6:cfp-10 fusion protein for differentiation of infections of cattle by mycobacterium bovis and by m.avium subsp. avium and m. avium subsp. paratuberculosis. Clinical and Diagnostic Laboratory Immunology. 11:729-735.

DESTRUCTION OF MYCOBACTERIUM PARATUBERCULOSIS, SALMONELLA SPP., AND MYCOPLASMA SSP. IN RAW MILK BY A COMMERCIAL ON FARM HIGH TEMPERATURE, SHORT-TIME PASTEURIZER - (Peer Reviewed Journal)

Stabel, J.R., Hurd, H.S., Calvente, S., Rosenbusch, R.F. 2004. Destruction of mycobacterium paratuberculosis, salmonella spp., and mycoplasma ssp. in raw milk by a commercial on farm high temperature, short-time pasteurizer. Journal of Dairy Science. 87(7):2177-83.

MYCOBACTERIUM PARATUBERCULOSIS: GENOME TO DISEASE CONTROL - (Abstract)

Stabel, J.R., Bannantine, J.P., Robbe Austerman, S., Paustian, M., Huntley, J. 2004. Mycobacterium paratuberculosis: genome to disease control[abstract]. Meeting Abstract.

EFFECTS OF INTERFERON-G, INTERLEUKIN-10, AND TRANSFORMING GROWTH FACTOR-B ON SURVIVAL OF MYCOBACTERIUM PARATUBERCULOSIS IN MONOCYTE-DERIVED MACROPHAGES FROM NATURALLY INFECTED CATTLE - (Abstract)

Stabel, J.R., Khalifeh, M.S. 2004. Effects of Interferon-g, Interleukin-10, and Transforming growth factor-b on survival of Mycobacterium paratuberculosis in Monocyte-derived Macrophages from naturally infected cattle [abstract]. Federation of American Societies for Experimental Biology Conference. p. 251.

UPREGULATION OF TRANSFORMING GROWTH FACTOR-BETA AND INTELEUKIN-10 IN COWS WITH CLINICAL JOHNE'S DISEASE - (Peer Reviewed Journal)

Khalifeh, M.S., Stabel, J.R. 2004. Upregulation of transforming growth factor-beta and inteleukin-10 in cows with clinical johne's disease. Veterinary Immunology and Immunopathology. 99(1-2):39-46.

EFFECTS OF INTERFERON-G, INTERLUKIN-10, AND TRANSFORMING GROWTH FACTOR-B ON THE SURVIVAL OF MYCOBACTERIUM PARATUBERCULOSIS IN MONOCYTE-DERIVED MACROPHAGES FROM NATURALLY INFECTED CATTLE - (Peer Reviewed Journal)

Khalifeh, M.S., Stabel, J.R. 2004. Effects of interferon-g, interlukin-10, and transforming growth factor-b on the survival of mycobacterium paratuberculosis in monocyte-derived macrophages from naturally infected cattle. Infection and Immunity. 72:1974-1982.

EXPERIMENTAL MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS INFECTION OF CALVES: EARLY INDUCTION OF A CONCURRENT HUMORA AND CELLULAR IMMUNE RESPONSE - (Abstract) - (02-Dec-03)

MODULATORY EFFECTS OF IFN-G, IL-10 AND TGF-B IN CATTLE INFECTED WITH MYCOBACTERIUM PARATUBERCULOSIS - (Abstract)

Stabel, J.R., Khalifeh, M.S. 2005. Modulatory Effects of IFN-g, IL-10 and TGF-b in cattle Infected with Mycobacterium paratuberculosis [abstract]. ARS Immunology Workshop. p. 51.

APPLICATION OF THE GENOME SEQUENCES TO ADDRESS CONCERNS THAT MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS MIGHT BE A FOODBORNE PATHOGEN - (Peer Reviewed Journal)

Bannantine, J.P., Barletta, R.G., Stabel, J.R., Paustian, M., Kapur, V. 2004. Application of the genome sequences to address concerns that mycobacterium avium subspecies paratuberculosis might be a foodborne pathogen. Foodborne Pathogens and Disease. 1:3-15.

COMPARISON OF ADHESION MOLECULE EXPRESSION ON LYMPHOCYTES FROM BLOOD AND MILK OF NORMAL COWS AND COWS WITH JOHNE'S DISEASE - (Abstract)

Harp, J.A., Stabel, J.R., Pesch, B.A., Goff, J.P. 2003. Comparison of adhesion molecule expression on lymphocytes from blood and milk of normal cows and cows with Johne's disease [abstract]. Conference Research Workers Disease Meeting. p. 71P.

EXPRESSION OF ADHESION MOLECULES ON MILK AND BLOOD LEUKOCYTES FROM PERIPARTURIENT DAIRY CATTLE WITH JOHNE'S DISEASE - (Peer Reviewed Journal)

Harp, J.A., Stabel, J.R., Pesch, B.A., Goff, J.P. 2004. Expression of adhesion molecules on milk and blood leukocytes from periparturient dairy cattle with Johne's disease. Veterinary Immunology and Immunopathology. 98(1-2):69-76.

COMPARISON OF THE G-INTERFERON ELISA AND THE SKIN TEST FOR THE DETECTION OF SUB-CLINICAL JOHNE'S DISEASE IN CATTLE - (Proceedings/Symposium) - (18-Sep-03)

EXPRESSIONAND IMMUNOGENICITY OF PROTEINS ENCODED BY SEQUENCES SPECIFIC TO MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS - (Peer Reviewed Journal)

Bannantine, J.P., Hansen, J.K., Paustian, M., Amonsin, A., Li, L.L., Stabel, J.R., Kapur, V. 2004. Expressionand immunogenicity of proteins encoded by sequences specific to mycobacterium avium subsp. paratuberculosis. Journal of Clinical Microbiology. 42:106-114.

EXPERIMENTAL MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS INFECTION OF CALVES: EARLY INDUCTION OF A HUMORAL AND CELLULAR IMMUNE RESPONSE - (Peer Reviewed Journal)

Waters, W.R., Miller, J.M., Palmer, M.V., Stabel, J.R., Jones, D.E., Koistinen, K.A., Steadham, E.M., Hamilton, M.J., Davis, W.C., Bannantine, J.P. 2003. Experimental mycobacterium avium subsp. paratuberculosis infection of calves: early induction of a humoral and cellular immune response. Infection and Immunity. 71:5130-5138.

EFFECTS OF SUPPLEMENTAL ENERGY ON METABOLIC PARAMETERS AND IMMUNE FUNCTION IN PERIPARTURIENT DAIRY COWS WITH JOHNE'S DISEASE - (Peer Reviewed Journal)

Stabel, J.R., Goff, J.P., Kimura, K. 2003. Effects of supplemental energy on metabolic parameters and immune function in periparturient dairy cows with johne's disease. Journal of Dairy Science. 86:3527-3535.

THE MYCOBACTERIUM AVIUM SUPSPECIES PARATUBERCULOSIS 35 KDA PROTEIN PLAYS A ROLE IN INVASION OF BOVINE EPITHELIAL CELLS - (Peer Reviewed Journal)

Bannantine, J.P., Huntley, J.F., Miltner, E., Stabel, J.R., Bermudez, L.E. 2003. The mycobacterium avium supspecies paratuberculosis 35 kda protein plays a role in invasion of bovine epithelial cells. Infection and Immunity.

A SIMPLE, RAPID, AND EFFECTIVE METHOD FOR THE EXTRACTION OF MYCOBACTERIUM PARATUBERCULOSIS DNA FROM FECAL SAMPLES FOR PCR - (Peer Reviewed Journal)

Stabel, J.R., Bosworth, T.L., Kirkbride, T.A., Forde, R.L., Whitlock, R.H. 2004. A simple, rapid, and effective method for the extraction of mycobacterium paratuberculosis dna from fecal samples for pcr. Journal of Veterinary Diagnostic Investigation. 16:22-30.

IMMUNE RESPONSES AFTER ORAL INOCULATION OF WEANLING BISON OR BEEF CALVES WITH A BISON OR CATTLE STRAIN OF MYCOBACTERIUM PARATUBERCULOSIS - (Peer Reviewed Journal)

Stabel, J.R., Palmer, M.V., Whitlock, R.H. 2003. Immune responses after oral inoculation of weanling bison or beef calves with a bison or cattle strain of mycobacterium paratuberculosis. Journal of Wildlife Diseases. 39:545-555.

MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS: EVIDENCE FOR LIMITED STRAIN DIVERSITY, STRAIN SHARING, AND IDENTIFICATION OF UNIQUE TARGETS FOR DIAGNOSIS - (Peer Reviewed Journal)

Motiwala, A.S., Strother, M., Amonsin, A., Byrum, B., Naser, S., Stabel, J.R., Shulaw, W., Bannantine, J.P., Kapur, V., Sreevatsan, S. 2003. Molecular epidemiology of mycobacterium avium subspecies paratuberculosis: evidence for limited strain diversity, strain sharing, and identification of unique targets for diagnosis. Journal of Clinical Microbiology.

EFFECTIVE METHODS FOR POSTHARVEST INTERVENTION IN DAIRY PROCESSING - (Peer Reviewed Journal)

Stabel, J.R. 2003. Effective Methods for Postharvest Intervention in Dairy Processing. Journal of Dairy Science. 86:E10-E15.