Pacific Northwest Research Station

Forest Genetics Team

2008 Science Accomplishments

Scientists develop cost-effective methods for genome sequencing

Vast improvements in DNA sequencing offer unprecedented insight into the connection between genomes and phenotypes-that is, the interactions between an organism's hereditary genetic information and its observable characteristics that may be shaped by its environment. Nevertheless, current sequencing methods emphasize sequencing "depth" (e.g., a genome from one individual) over "breadth" (part of a genome from hundreds of individuals), making them poorly suited to population studies.

To address this need, station geneticists developed cost-effective methods to sequence DNA for population-level studies. Using this "multiplex-sequencing-by-synthesis" approach, it is possible to simultaneously sequence 150 chloroplast genomes (plants), 1,100 mitochondrial genomes (animals), or 10,000 genes from an individual in a single sequencing run.

Station geneticists are using these methods to characterize genetic diversity in threatened species, such as the Torrey pine, and to evaluate gene flow in native and managed stands of conifers. They are also using them to examine historical migration in pine species, the role of mutation on adaptation to climate in conifers, host and pathogen interactions, and to categorize taxonomically challenging species based on genetic similarities.

Partners: Oregon State University, Santa Clara University, Universidad Nacional Autónoma de México

To learn more, contact Richard Cronn at rcronn@fs.fed.us.

Scientists map seed zones for oceanspray based on climatic variables

Land managers generally try to use locally adapted and genetically diverse plant materials in restoration and revegetation efforts. Oceanspray (Holodiscus discolor) is an important restoration shrub in the Pacific Northwest; genetic variation exists within the species because local populations adapt to local conditions. In this study, station geneticists identified the patterns of genetic diversity of oceanspray related to climatic variables, such as average December minimum temperature, by characterizing the variability in growth and physical characteristics for individual shrubs from 39 locations in western Oregon and Washington.

As part of this study, scientists produced a seed zone map for land managers that illustrates adaptive patterns of genetic diversity of oceanspray within the Pacific Northwest. The map can be used to guide seed collection and deployment for native plant restoration and revegetation activities.

Partners: Corvallis Plant Materials Center and Tangent Service Center, USDA Natural Resources Conservation Service

To learn more, contact Matt Horning at mhorning@fs.fed.us.

Climate change task force begins evaluating genetic and silvicultural options

Station scientists and their colleagues at Oregon State University have formed a task force of forest managers, forest geneticists, tree breeders, silviculturists, and tree physiologists to explore management options for adapting to the effects of climate change on forests in the Western United States.

As part of its outreach education efforts, the task force developed a Web site to inform the public about the potential effects of climate change and forest management options. The site has links to relevant publications, presentations, and upcoming workshops.

Partner: Oregon State University

To learn more, contact Brad St. Clair at bstclair@fs.fed.us.

For more information: See http://tafcc.forestry.oregonstate.edu/index.html.