Quantitative Proteomics Analysis of Aberrant Molecular Events in Acute Myeloid Leukemia |
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Launch in standalone player | |
Air date: | Thursday, December 04, 2008, 10:00:00 AM |
Category: | Proteomics |
Runtime: | 75 minutes |
NLM Title: | Quantitative proteomics analysis of aberrant molecular events in acute myeloid leukemia [electronic resource] / Jarrod A. Marto. |
Author: | Marto, Jarrod A. National Institutes of Health (U.S.). Proteomics Interest Group. |
Publisher: | [Bethesda, Md. : National Institutes of Health, 2008] |
Abstract: | (CIT): Acute myeloid leukemia (AML) remains a highly lethal malignancy with limited therapeutic options. Full transformation in AML requires coupling of aberrant molecular events associated with multiple cellular processes. For example, mutation of the FLT-3 receptor tyrosine kinase is frequently observed in AML and drives abnormal proliferation. Signaling from oncogenic FLT-3 phosphorylates the transcription factor C/EBP [alpha] and leads to disrupted myeloid differentiation. We are using iTRAQ-based quantitative proteomics approaches to explore divergent signaling downstream of two common FLT-3 mutants, and to monitor remodeling of C/EBP [alpha] protein complexes between transcriptionally competent and repressed states. Representative proteomics data and biochemical results will be described, in addition to and methodological improvements in proteomics-related technologies. |
Subjects: | Leukemia, Myeloid, Acute--genetics Models, Molecular Proteomics--methods fms-Like Tyrosine Kinase 3--genetics |
Publication Types: | Government Publications Lectures |
NLM Classification: | QZ 350 |
NLM ID: | 101493100 |
CIT File ID: | 14811 |
CIT Live ID: | 7331 |
Permanent link: | http://videocast.nih.gov/launch.asp?14811 |