Projects

CHARACTERIZATION & EPIDEMIOLOGY OF CITRUS TRISTEZA VIRUS & OTHER INVASIVE & EMERGING GRAFT-TRANSMISSIBLE DISEASES OF CITRUS IN CALIFORNIA Appropriated (D)   Accession Number: 411528

CITRUS STUBBORN DISEASE IN CALIFORNIA: EPIDEMIOLOGY OF A RE-EMERGING PATHOGEN AND ITS VECTORS Specific Cooperative Agreement (S)   Accession Number: 409546

INVESTIGATION OF SEEDLING YELLOWS CROSS PROTECTION BY MILD COMPONENTS OF DEKOPON CITRUS TRISTEZA VIRUS STRAIN Trust (T)   Accession Number: 415263

OPTIMIZATION AND APPLICATION OF REAL TIME PCR FOR THE DETECTION OF SPIROPLASMA CITRI IN CITRUS NURSERY STOCK AND GROVES Trust (T)   Accession Number: 413150

Publications

Elevated Background in double antibody sandwich-indirect enzyme-linked immunosorbent assay for the detection of Citrus tristeza virus in mandarin cultivars - (Peer Reviewed Journal) - (12-Mar-09)

Estimation of Citrus Stubborn Disease Incidence in Citrus Groves by real-time PCR - (Peer Reviewed Journal) - (17-Aug-08)

Differential stylet penetration behaviors of two Aphis gossypii biotypes in relation to host or vector infection with Citrus tristeza virus - (Abstract)

Gutierrez, J.D., Backus, E.A., Yokomi, R.K., Schreiber, F. 2008. Differential stylet penetration behaviors of two Aphis gossypii biotypes in relation to host or vector infection with Citrus tristeza virus. Entomological Society of America Annual Meeting. Paper No. 36418.

Assessment of Stubborn Disease Incidence in Citrus - (Peer Reviewed Journal) - (29-Apr-08)

Genetic Diversity of Spiroplasma citri strains from Different Regions, Hosts, and Isolation Dates - (Peer Reviewed Journal)

Mello,, A.F., Yokomi, R.K., Mulcher,, U., Chen, J., Wayadande,, A., Fletcher, J. 2008. Genetic Diversity of Spiroplasma citri strains from Different Regions, Hosts, and Isolation Dates. Phytopathology. 98:960-968.

Citrus stubborn disease incidence determined by quantitative real time PCR - (Abstract)

Yokomi, R.K., Mello,, A.F., Fletcher,, J. 2008. Citrus stubborn disease incidence determined by quantitative real time PCR. Journal of Plant Pathology 90 (2, Suppl.) S2.465.

Molecular analyses revealed genetic complexity in Citrus tristeza virus Dekopon isolate and its aphid-transmitted progeny - (Abstract)

Yokomi, R.K., Saponari,, M., Weng,, Z., Xiong,, Z. 2008. Molecular analyses revealed genetic complexity in Citrus tristeza virus Dekopon isolate and its aphid-transmitted progeny [abstract]. Journal of Plant Pathology 90(2, Suppl.),S2.314.

Transmission of Spiroplasma citri to Carrots by Circulifer tenellus - (Abstract)

Mello,, A.F., Yokomi, R.K., Chen, J., Wayadande,, A., Fletcher,, J. 2008. Transmission of Spiroplasma citri to Carrots by Circulifer tenellus. Journal of Plant Pathology 90(2, Suppl.), S2.463.

PCR-based Detection of Spiroplasma Citri Associated with Citrus Stubborn Disease - (Peer Reviewed Journal)

Yokomi, R.K., Mello, A., Saponari, M., Fletcher, J. 2008. PCR-based detection of spiroplasma citri associated with citrus stubborn disease. Plant Disease. 92:253-260.

Quantitative Detection of Spiroplasma Citri by Real Time PCR - (Abstract)

Yokomi, R.K., Mello,, A.F., Fletcher,, J., Saponari, M. 2007. Quantitative Detection of Spiroplasma Citri by Real Time PCR. In: Proceedings of the 17th Conference International Organization of Citrus Virologists, October 22-26, 2007, Adana, Turkey. p. 172.

Elevated Background in DAS-I ELISA for the Detection of Citrus Tristeza Virus in Mandarin Varieties - (Abstract)

Yokomi, R.K., Polek,, M. 2007. Elevated Background in DAS-I ELISA for the Detection of Citrus Tristeza Virus in Mandarin Varieties. In: Proceedings of the 17th Conference International Organization of Citrus Virologists, October 22-26, 2007, Adana, Turkey. p. 66.

Rapid Assessment of the Citrus Tristeza Virus Isolates Detected in Spring 2007 at the Lindcove Research and Extension Center, Exeter, Calif. - (Abstract)

Yokomi, R.K., Polek,, M., Grafton-Cardwell,, B., O'Connell,, N. 2007. Rapid Assessment of the Citrus Tristeza Virus Isolates Detected in Spring 2007 at the Lindcove Research and Extension Center, Exeter, California. In: Proceedings of the 17th Conference of International Organization of Citrus Virologists, October 22-26, 2007, Adana, Turkey. p. 58.

Biological and Molecular Characterization of Two Virulent CTV Isolates Found in Central California - (Abstract)

Yokomi, R.K., Saponari,, M. 2007. Biological and Molecular Characterization of Two Virulent CTV Isolates Found in Central California. In: Proceedings of the 17th International Organization of Citrus Virologists. p. 71.

Characterization of Citrus Tristeza Virus Isolates by Single-strand Conformation Polymorphism Analysis of the Coat Protein Gene - (Abstract)

Saponari,, M., Yokomi, R.K. 2007. Characterization of Citrus Tristeza Virus Isolates by Single-strand Conformation Polymorphism Analysis of the Coat Protein Gene. In: Proceedings of the 17th International Organization of Citrus Virologists, October 22-26, 2007, Adana, Turkey. p. 139

Use of the CP and CPm Intergene Sequences to Discriminate CTV Strains - (Abstract)

Saponari,, M., Yokomi, R.K. 2007. Use of the CP and CPm Intergene Sequences to Discriminate CTV Strains. In: Proceedings of the 17th International Organization of Citrus Virologists, October 22-26, 2007, Adana, Turkey. p. 138.

Assessment of Stubborn Disease Incidence in Citrus - (Abstract)

Mello,, A.F., Yokomi, R.K., Fletcher,, J. 2007. Assessment of Stubborn Disease Incidence in Citrus. In: Proceedings of the 17th International Organization of Citrus Virologists, October 22-26, 2007, Adana, Turkey. p. 173.

Real-time RT-PCR assay for detection and differentiation of Citrus tristeza virus isolates - (Abstract)

Saponari,, M., Yokomi, R.K. 2007. Real-time RT-PCR assay for detection and differentiation of Citrus tristeza virus isolates. Journal of Plant Pathology 89 (3, supplement):S60.

Quantitative Detection of Citrus tristeza virus (CTV) in Citrus and Aphids by Real-time Reverse Transcription-PCR (TaqMan®) - (Peer Reviewed Journal)

Saponari, M., Keremane, M.L., Yokomi, R.K. 2008. Quantitative Detection of Citrus tristeza virus (CTV) in Citrus and Aphids by Real-time Reverse Transcription-PCR (TaqMan®). Journal of Virological Methods. 147:43-53.

PCR-based Detection of Spiroplasma citri Associated with Citrus Stubborn Disease - (Abstract)

Yokomi, R.K., Mello, A.F., Saponari, M., Fletcher, J. 2007. PCR-based Detection of Spiroplasma citri Associated with Citrus Stubborn Disease. Phytopathology. 97:S127.

Real-time RT-PCR Assay for Detection and Differentiation of Citrus Tristeza Virus Isolates - (Abstract)

Saponari, M., Yokomi, R.K. 2007. Real-time RT-PCR Assay for Detection and Differentiation of Citrus Tristeza Virus Isolates. Phytopathology. 97:S104.

Genetic Diversity of Spiroplasma citri Isolates from Different Geographical Regions, Plant Hosts, and Dates of Isolations - (Abstract)

Mello, A.F., Yokomi, R.K., Melcher, U., Chen, J., Fletcher, J. 2007. Genetic Diversity of Spiroplasma citri Isolates from Different Geographical Regions, Plant Hosts, and Dates of Isolations. Phytopathology. 97:S75.

Spiroplasma citri Infection Affects Yield and Fruit Quality in Commercial Citrus Grove in California - (Abstract)

Mello, A.F., Fletcher, J., Yokomi, R.K. 2007. Spiroplasma citri Infection Affects Yield and Fruit Quality in Commercial Citrus Grove in California. Phytopathology. 97:S74.

IDENTIFICATION OF INFECTION LOCI OF SPIROPLASMA CITRI IN THE SAN JOAQUIN VALLEY. - (Abstract)

Yokomi, R.K., Mello, A., Fletcher, J., Chen, J.C. 2006. Identification of infection loci of Spiroplasma citri in the San Joaquin Valley. Phytopathology 97:S172. APS Net. 2006 Pacific Division Meeting Abstracts, June 13-16, 2006 - Boise, ID. Posted online Oct. 2, 2006. http://www.apsnet.org/ meetings/div/pc06abs.asp.

MOLECULAR TECHNIQUES TO ASSESS GENETIC DIVERSITY AMONG ISOLATES OF SPIROPLASMA CITRI. - (Abstract)

Mello, A.F.S., Yokomi, R.K., Melcher, U., Chen, J.C., Fletcher. J. 2006. Molecular techniques to assess genetic diversity among isolates of Spiroplasma citri [abstract]. 16th International Congress International Organization for Mycoplasmology (IOM), St John's College Cambridge, UK, July 9-14, 2006.

ASSESSMENT OF GENETIC DIVERSITY IN SPIROPLASMA CITRI BY RAPD, REP-PCR AND BACTERIOPHAGE PRIMERS - (Abstract)

Mello, A.F., Yokomi, R.K., Melcher, U., Chen, J., Fletcher, J. 2006. Assessment of Genetic Diversity in Spiroplasma citri by RAPD, rep-PCR and Bacteriophage Primers. Meeting Abstract. Phytopathology 96:S77

MULTIPLEX TAQMAN-BASED PCR FOR SENSITIVE AND ACCURATE QUANTIFICATION OF CITRUS HLB AND CVC PATHOGENS; CANDIDATUS LIBERIBACTER AND XYLELLA FASTIDIOSA - (Abstract)

Lin, H., Doddapaneni, H., Civerolo, E.L., Yao, J., Yokomi, R.K., Bai, X., Cao, H., Zhao, X., Wen, R. 2006. Multiplex taqman-based pcr for sensitive and accurate quantification of citrus hlb and cvc pathogens; candidatus liberibacter and xylella fastidiosa [Abstract]. Proceedings of the Huanglongbing-greening International Workshop. p. 83.

INITIAL SURVEY FOR SPIROPLASMA CITRI, A LEAFHOPPER-TRANSMITTED PATHOGEN INFECTING CITRUS AND OTHER CROPS IN CENTRAL CALIFORNIA. - (Abstract)

Yokomi, R.K., Mello, A., Kallsen, C., Nunez, J., Gorden, J., O¿Connell, N., Freeman, M., Chen, J.C., Fletcher, J. 2006. Initial survey for Spiroplasma citri, a leafhopper-transmitted pathogen infecting citrus and other crops in central California [abstract]. In: Abstracts 90th Annual Meeting Pacific Branch ESA, Maui, Hawaii, March 5-8, 2006. p.108-109.

TRANSMISSION AND SPREAD OF CITRUS TRISTEZA VIRUS IN CENTRAL CALIFORNIA. - (Book/Chapter) - (10-Aug-05)

BIOCHARCTERIZATION OF AN INTERNATIONAL COLLECTION OF CITRUS TRISTEZA VIRUS (CTV) ISOLATES. - (Abstract) - (04-Jul-05)

BIOLOGICAL CHARACTERIZATION OF AN INTERNATIONAL COLLECTION OF CITRUS TRISTEZA VIRUS (CTV) ISOLATES - (Proceedings/Symposium) - (04-Jul-05)

INCIDENCE, TRANSMISSIBILITY, AND GENOTYPE OF CITRUS TRISTEZA VIRUS (CTV) ISOLATES FROM A CTV ERADICATIVE AND A NON-ERADICATIVE DISTRICT IN CENTRAL CALIFORNIA - (Peer Reviewed Journal)

Yokomi, R.K., Deborde, R.L. 2005. Incidence, transmissibility, and genotype of citrus tristeza virus (CTV) isolates from a CTV eradicative and a non-eradicative district in central california. Plant Disease. 89(8):859-866.

AMINO ACID SEQUENCE VARIATIONS ASSOICATED WITH APHID TRANSMISSIBILITY OF CITRUS TRISTEZA VIRUS. - (Abstract)

Roy, G., Yokomi, R.K., Ullman, D. 2005. Amino acid sequence variations assoicated with aphid transmissibility of citrus tristeza virus.[Abstract]. Phytopathology. 95:S91.

TRANSMISSION PROFILES OF CALIFORNIA CITRUS TRISTEZA VIRUS ISOLATES BY THE COTTON APHID, APHIS GOSSYPII (HOMOPTERA: APHIDIDAE). - (Abstract)

Yokomi, R.K. 2005. Transmission profiles of California Citrus tristeza virus isolates by the cotton aphid, Aphis gossypii (homoptera: aphididae). Entomology Society of America Pacific Branch 89th Annual Meeting. p.103

BIOCHARACTERIZATION OF AN INTERNATIONAL COLLECTION OF CITRUS TRISTEZA VIRUS (CTV) ISOLATES - (Abstract)

Garnsey, S.M., Civerolo, E.L., Gumpf, D.J., Paul, C., Hilf, M.E., Lee, R.F., Brlanskyr, R.H., Yokomi, R.K., Hartung, J.S. 2004. Biocharacterization of an international collection of citrus tristeza virus (CTR) isolates. International Organization of Citrus Virologists Abstracts, Page 49.

BIOLOGICAL CHARACTERIZATION OF AN INTERNATIONAL COLLECTION OF CITRUS TRISTEZA VIRUS (CTV)ISOLATES - (Peer Reviewed Journal)

Garnsey, S.M., Civerolo, E.L., Gumpf, D.J., Paul, C., Hilf, M.E., Lee, R.F., Brlansky, R.H., Yokomi, R.K., Hartung, J.S. 2007. Biological characterization of an international collection of citrus tristeza virus (ctv)isolates. In: Proceedings of Conference of International Organization of Citrus Virologists. p. 75-93.

PRELIMINARY EVALUATION OF TWO BIOTYPES OF APHIS GOSSYPII ON THE TRANSMISSION OF CITRUS TRISTEZA VIRUS. - (Abstract)

Yokomi, R.K., Joost, J.H., Backus, E.A. 2004. Preliminary evaluation of two biotypes of aphis gossypii on the transmission of citrus tristeza virus. [Abstract] Conference of International Organization of Citrus Virologists. p.136.

TRANSMISSIBILITY AND GENOTYPE ANALYSIS OF CENTRAL CALIFORNIA ISOLATES OF CITRUS TRISTEZA CLOSTEROVIRUS - (Abstract)

Yokomi, R.K. 2004. Transmissibility and genotype analysis of Central California isolates of Citrus tristeza closterovirus. Phytopathology 94:S-131.