Projects

TRANSMISSION, DIFFERENTIATION, AND PATHOBIOLOGY OF TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES Appropriated (D)   Accession Number: 411467

PRODUCTION OF TRANSGENIC MICE Specific Cooperative Agreement (S)   Accession Number: 412474

BSE PATHOGENESIS STUDY Specific Cooperative Agreement (S)   Accession Number: 408830

STUDY OF ATYPICAL BSE Specific Cooperative Agreement (S)   Accession Number: 408490

SUSCEPTIBILITY OF CATTLE WITH THE E211K PRNP ALLELE TO BSE Specific Cooperative Agreement (S)   Accession Number: 413249

DETECTION OF PRP**D IN TISSUE SAMPLES AND BODILY FLUIDS OF CATTLE FROM THE GERMAN BSE PATHOGENESIS STUDY Specific Cooperative Agreement (S)   Accession Number: 411017

Publications

Characterization of a U.S. Sheep Scrapie Isolate with Short Incubation Time - (Peer Reviewed Journal) - (16-Apr-09)

Origins of BSE: evidence of sporadic and heritable BSE - (Abstract)

Nicholson, E.M., Brunelle, B.W., Kehrli, Jr., M.E., Greenlee, J.J. 2008. Origins of BSE: Evidence of Sporadic and Heritable BSE [abstract]. United States-Japan Cooperative Program in Natural Resources. p. 7.

Experimental transmission of U.S. scrapie agent to neonatal sheep by oral route - (Peer Reviewed Journal)

Hamir, A.N., Kunkle, R.A., Greenlee, J.J., Richt, J.A. 2009. Experimental Oral Transmission of United States Origin Scrapie to Neonatal Sheep. Journal of Veterinary Diagnostic Investigation. 21(1):64-68.

Frequencies of polymorphisms associated with BSE resistance differ significantly between Bos taurus, Bos indicus, and composite cattle - (Peer Reviewed Journal)

Brunelle, B.W., Greenlee, J.J., Seabury, C.M., Brown Ii, C.E., Nicholson, E.M. 2008. Frequencies of polymorphisms associated with BSE resistance differ significantly between Bos taurus, Bos indicus, and composite cattle. BioMed Central (BMC) Veterinary Research. 4(1):36. Available:http://www.biomedcentral.com/1746-6148/4/36.

Serial passage of sheep scrapie inoculum in Suffolk sheep - (Peer Reviewed Journal)

Hamir, A.N., Kunkle, R.A., Richt, J.A., Greenlee, J.J., Miller, J.M. 2009. Serial Passage of Sheep Scrapie Inoculum in Suffolk Sheep. Veterinary Pathology. 46(1):39-44.

Strain typing of U.S. scrapie strains using a panel of inbred mice - (Abstract)

Greenlee, J.J., Kunkle, R.A., Nicholson, E.M., Hamir, A.N., Bulgin, M.S., Richt, J. 2009. Strain Typing of U.S. Scrapie Strains Using a Panel of Inbred Mice [abstract]. American College of Veterinary Pathologists. Paper No. 121. p. 762.

Experimental transmission of transmissible spongiform encephalopathies (scrapie, chronic wasting disease, transmissible mink encephalopathy) to cattle and their differentiation from bovine spongiform encephalopathy - (Abstract)

Hamir, A.N., Cutlip, R.C., Miler, J.M., Kunkle, R.A., Richt, J.A., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2008. Experimental Transmission of Transmissible Spongiform Encephalopathies (Scrapie, Chronic Wasting Disease, Transmissible Mink Encephalopathy) to Cattle and Their Differentiation from Bovine Spongiform Encephalopathy [abstract]. American College of Veterinary Pathologists. Paper No. 153. p. 770.

Identification of a heritable polymorphism in bovine PRNP associated with genetic transmissible spongiform encephalopathy: evidence of heritable BSE - (Peer Reviewed Journal)

Nicholson, E.M., Brunelle, B.W., Richt, J.A., Kehrli, Jr., M.E., Greenlee, J.J. 2008. Identification of a Heritable Polymorphism in Bovine PRNP Associated with Genetic Transmissible Spongiform Encephalopathy: Evidence of Heritable BSE. PloS ONE. 3(8): e2912.

Experimental transmission of transmissible spongiform encephalopathies (scrapie, chronic wasting disease, transmissible mink encephalopathy) to cattle and their differentiation from bovine spongiform encephalopathy - (Abstract)

Hamir, A.N., Kunkle, R.A., Richt, J.A., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2008. Experimental Transmission of Transmissible Spongiform Encephalopathies (Scrapie, Chronic Wasting Disease, Transmissible Mink Encephalopathy) to Cattle and their Differentiation from Bovine Spongiform Encephalopathy [abstract]. Prion 2008. p. 65.

Lack of prion accumulation in lymphoid tissues of scrapie-affected sheep with AA136, QR171 polymorphism type - (Abstract)

Kunkle, R.A., Hamir, A.N., Richt, J.A., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2008. Lack of Prion Accumulation in Lymphoid Tissues of Scrapie-Affected Sheep with AA136, QR171 Polymorphism Type [abtract]. Prion 2008. p. 75.

Identification of a heritable polymorphism in the bovine prion gene associated with genetic transmissible spongiform encephalopathy: evidence of heritable BSE - (Abstract)

Nicholson, E.M., Brunelle, B.W., Richt, J.A., Kehrli, Jr., M.E., Greenlee, J.J. 2008. Identification of a Heritable Polymorphism in the Bovine Prion Gene Associated with Genetic Transmissible Spongiform Encephalopathy: Evidence of Heritable BSE [abstract]. Prion 2008. p. 114.

Western-blot detection of PrP**sc in archived paraffin-embedded brainstem from scrapie-affected sheep - (Peer Reviewed Journal)

Kunkle, R.A., Nicholson, E.M., Lebepe-Mazur, S., Orcutt, D.L., Srinivas, M.L., Greenlee, J.J., Alt, D.P., Hamir, A.N. 2008. Western blot detection of PrP**sc in archived paraffin-embedded brainstem from scrapie-affected sheep. Journal of Veterinary Diagnostic Investigation. 20(4):522-526.

Experimental transmission of chronic wasting disease (CWD) of elk (Cervus elaphus nelsoni), white-tailed deer (Odocoileus virginianus), and mule deer (Odocoileus hemionus hemionus) to white-tailed deer by intracerebral route - (Peer Reviewed Journal)

Hamir, A.N., Richt, J., Miller, J.M., Kunkle, R.A., Hall, S., Nicholson, E.M., Orourke, K.I., Greenlee, J.J., Williams, E.S. 2008. Experimental transmission of chronic wasting disease (CWD) of elk (Cervus elaphus nelsoni), white-tailed deer (Odocoileus virginianus), and mule deer (Odocoileus hemionus hemionus) to white-tailed deer by intracerebral route. Veterinary Pathology. 45(3):297-306.

Prolonged CWD incubation time and unique PrP**d profile in Prnp 132LL elk - (Abstract)

Greenlee, J.J., O'Rourke, K.I., Hamir, A.N., Gidlewski, T., Zhuang, D., Spraker, T. 2007. Prolonged CWD Incubation Time and Unique PrP**d Profile in Prnp 132LL Elk. In: Proceedings of the U.S. Animal Health Association 111th Annual Meeting, Captive Wildlife and Alternative Livestock Committee, October 20, 2007, Reno, Nevada. p. 264-265.

Retinal cell types are differentially affected in sheep with scrapie - (Peer Reviewed Journal)

Smith, J.D., Greenlee, J.J., Hamir, A.N., West Greenlee, M.H. 2008. Retinal cell types are differentially affected in sheep with scrapie. Journal of Comparative Pathology. 138(1):12-22.

Elk with a long incubation prion disease phenotype have a unique PrP-d profile - (Peer Reviewed Journal)

O'Rourke, K.I., Spraker, T.R., Zhuang, D., Greenlee, J.J., Gidlewski, T.E., Hamir, A.N. 2007. Elk with a long incubation prion disease phenotype have a unique PrP-d profile. NeuroReport. 18(18):1935-1938.

Experimental transmission of U.S. scrapie agent by nasal, peritoneal and conjunctival routes to genetically susceptible sheep - (Peer Reviewed Journal)

Hamir, A.N., Kunkle, R.A., Richt, J.A., Miller, J.M., Greenlee, J.J. 2008. Experimental transmission of US scrapie agent by nasal, peritoneal, and conjunctival routes to genetically susceptible sheep. Veterinary Pathology. 45(1):7-11.

Abnormal prion protein in the retina of Rocky Mountain elk (Cervus Elaphus Nelsoni) - (Abstract)

Spraker, T, Greenlee, J., Gidlewski T., O¿Rourke, K. 2007. Abnormal Prion Protein in the Retina of Rocky Mountain Elk (Cervus Elaphus Nelsoni) [abstract]. Prion 2007. Paper No. P03.70. p. 64.

Experimental transmission of transmissible spongiform encephalopathies (scrapie, chronic wasting disease, transmissible mink encephalopathy) to cattle and their differentiation from bovine spongiform encephalopathy - (Proceedings/Symposium)

Hamir, A.N., Cutlip, R.C., Miller, J.M., Kunkle, R.A., Richt, J.A., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2007. Experimental transmission of transmissible spongiform encephalopathies (scrapie, chronic wasting disease, transmissible mink encephalopathy) to cattle and their differentiation from bovine spongiform encephalopathy. In: Proceedings of the World Association of Veterinary Laboratory Diagnosticians 13th International Symposium, November 11-14, 2007, Melbourne, Australia. p. 29.

Detection of PrP**CWD in retinal tissues in white-tailed deer (Odocoileus virginianus) and Rocky Mountain elk (Cervus elaphus nelsoni) with CWD - (Abstract)

Spraker, T., Gidleweski, T., Greenlee, J., Keane, D., Hamir, A., Orourke, K. 2007. Detection of PrP**CWD in retinal tissues in white-tailed deer (Odocoileus virginianus) and Rocky Mountain elk (Cervus elaphus nelsoni) with CWD [abstract]. American Association of Veterinary Laboratory Diagnosticians 50th Annual Meeting. p. 47.

Cross-species transmission of TSE - (Proceedings/Symposium)

Richt, J.A., Hamir, A.N., Kunkle, R.A., Nicholson, E.M., Greenlee, J.J. 2007. Cross-species transmission of TSE. In: Proceedings of the American College of Veterinary Internal Medicine. 2007 ACVIM Forum, June 6-9, 2007, Seattle, Washington. Paper No. 160.

EXPERIMENTAL TRANSMISSION OF CHRONIC WASTING DISEASE (CWD) OF ELK (CERVUS ELAPHUS NELSONI), WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS), AND MULE DEER (ODOCOILEUS HEMIONUS HEMIONUS) TO WHITE-TAILED DEER BY INTRACEREBRAL ROUTE - (Abstract)

Kunkle, R.A., Hamir, A.N., Miller, J.M., Hall, S.M., Richt, J.A., Greenlee, J.J., Williams, E.S. 2006. Experimental transmission of chronic wasting disease (CWD) of elk (Cervus Elaphus Nelsoni), white-tailed deer (Odocoileus Virginianus), and mule deer (Odocoileus Hemionus Hemionus) to white-tailed deer by intracerebral route [abstract]. United States Animal Health Association. p. 221.

ATTEMPTED TRANSMISSION OF SCRAPIE AND CWD TO SWINE: PRELIMINARY FINDINGS - (Proceedings/Symposium)

Greenlee, J.J., Kunkle, R.A. Nicholson, E.M., Lager, K.M., Hamir, A.N. 2006. Attempted transmission of scrapie and CWD to swine: preliminary findings. 19th International Pig Veterinary Society Congress. p. 386.

ABNORMAL PRION ACCUMULATION ASSOCIATED WITH RETINAL PATHOLOGY IN EXPERIMENTALLY INOCULATED SCRAPIE-AFFECTED SHEEP - (Peer Reviewed Journal)

Greenlee, J.J., Hamir, A.N., West Greenlee, M.H. 2006. Abnormal prion accumulation associated with retinal pathology in experimentally inoculated scrapie-affected sheep. Veterinary Pathology. 43:733-739.

EXPERIMENTAL TRANSMISSION OF TRANSMISSIBLE MINK ENCEPHALOPATHY (TME) TO CATTLE BY INTRACEREBRAL INOCULATION - (Abstract)

Hamir, A.N., Kunkle, R.A., Miller, J.M., Greenlee, J.J., Richt, J.A. 2006. Experimental transmission of transmissible mink encephalopathy (TME) to cattle by intracerebral inoculation [abstract]. 4th International Veterinary Vaccines and Diagnostics Conference. p. 89. Paper No. PO53.

CROSS-SPECIES TSE TRANSMISSION STUDIES AT NADC - (Abstract)

Hamir, A.N., Cutlip, R.C., Miller, J.M., Kunkle, R.A., Greenlee, J.J., Richt, J.A. 2006. Cross-species TSE transmission studies at NADC [abstract]. 4th International Veterinary Vaccines and Diagnostics Conference. p. 89. Paper No. PO52.

EXPERIMENTAL INTRACEREBRAL AND ORAL INOCULATION OF SCRAPIE TO SWINE: PRELIMINARY REPORT - (Abstract)

Greenlee, J.J., Kunkle, R.A., Hamir, A.N. 2005. Experimental intracerebral and oral inoculation of scrapie to swine: preliminary report [abstract]. Proceedings of the American Association of Veterinary Laboratory Diagnosticians 48th Annual Conference. p. 38.

NATURAL AND EXPERIMENTAL PRION DISEASES OF CATTLE - (Abstract)

Richt, J.A., Hamir, A.N., Kunkle, R.A., Nicholson, E.M., Greenlee, J.J., Miller, J.M., Cutlip, R., Davis, A.J., Hall, S.M. 2005. Natural and experimental prion diseases of cattle [abstract]. Third International Rushmore Conference on Enteric Diseases. Paper No. 14.

EXPERIMENTAL SECOND PASSAGE OF CHRONIC WASTING DISEASE (CWD(MULE DEER)) AGENT TO CATTLE - (Peer Reviewed Journal)

Hamir, A.N., Kunkle, R.A., Miller, J.M., Greenlee, J.J., Richt, J.A. 2006. Experimental second passage of chronic wasting disease (CWD(mule deer)) agent to cattle. Journal of Comparative Pathology. 134(1):63-69.

EXPERIMENTAL TRANSMISSION OF TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES (TSE) AT THE NATIONAL ANIMAL DISEASE CENTER, AMES, IOWA: AN UPDATE - (Abstract)

Hamir, A.N., Cutlip, R.C., Miller, J.M., Kunkle, R.A., Greenlee, J.J., Richt, J.A. 2005. Experimental transmission of transmissible spongiform encephalopathies (TSE) at the National Animal Disease Center, Ames, Iowa: an update [abstract]. American Association of Veterinary Laboratory Diagnosticians. p. 169.

BIOCHEMISTRY OF PRION DISEASES - (Abstract)

Nicholson, E.M., Greenlee, J.J., Richt, J., Hamir, A.N., Kunkle, R.A., Kehrli, Jr., M.E. 2005. Biochemistry of prion diseases. Association of Analytical Communities International, Midwest Section [abstract]. Abstract No. 601. p. 38.

EXPERIMENTAL TRANSMISSION OF TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES (TSES) AT THE NATIONAL ANIMAL DISEASE CENTER (NADC), AMES, IOWA, USA: AN UPDATE - (Abstract)

Hamir, A.N., Cutlip, R.C., Miller, J.M., Kunkle, R.A., Greenlee, J.J., Richt, J.A., Bartz, J. 2005. Experimental transmission of transmissible spongiform encephalopathies (TSEs) at the National Animal Disease Center (NADC), Ames, Iowa, USA: An update [abstract]. Annual Wildlife Disease Association Conference. p. 263.

EXPERIMENTAL CANINE LEPTOSPIROSIS CAUSED BY LEPTOSPIRA INTERROGANS SEROVARS POMONA AND BRATISLAVA - (Peer Reviewed Journal)

Greenlee, J.J., Alt, D.P., Bolin, C.A., Zuerner, R.L., Andreasen, C.B. 2005. Experimental canine Leptospirosis caused by Leptospira interrogans serovars Pomona and Bratislava. American Journal of Veterinary Research. 66(10):1816-1822.

LOSS OF PRION PROTEIN IMMUNOREACTIVITY AFTER BOILING IN SATURATED CALCIUM HYDROXIDE SOLUTION - (Abstract)

Greenlee, J.J., Nicholson, E.M., Hamir, A.N., Richt, J.A., Kunkle, R.A., Noyes, G., Hotzapple, M.T., Kehrli Jr., M.E. 2005. Loss of prion protein immunoreactivity after boiling in saturated calcium hydroxide solution [abstract]. Keystone Symposia: Molecular Mechanisms of Transmissible Spongiform Encephalopathies. Poster No. 125.