Project Number: 1265-12630-003-13
Project Type: Nonfunded Cooperative Agreement

Start Date: Dec 04, 2008
End Date: Sep 30, 2013

Objective:
The objectives of this research are three-fold: (i) to detect and quantify pathogenic Escherichia coli (e.g. E coli 0157) in the Anacostia River, and (ii) to develop a fecal source tracking approach with respect to the upstream fecal contamination problem in the Anacostia River, and (iii) to monitor water quality on fecal coliform in the downstream portion of the Anacostia River.

Approach:
Samples of the Anacostia River water will be obtained by the Anacostia Watershed Society (AWS) staff. These samples will be analyzed for pathogenic E. coli using immunological and genetic methods previously developed by USDA scientists. Staff from the AWS will perform testing in regards to fecal coliform and other parameters for their survey. ARS will provide the use of its laboratory equipment to AWS. AWS will supply reagents for parameters of their own testing. Participation in the partnership by the AWS is contingent upon the ability to obtain funding for this program from outside sources in order to satisfy the financial obligations incurred by AWS for this program.