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Research Project:
Assay Development for Detection of Pathogenic E. Coli in Environmental Samples
Location: Environmental Microbial and Food Safety Laboratory
Project Number: 1265-12630-003-12
Project Type:
Nonfunded Cooperative Agreement
Start Date: Mar 06, 2008
End Date: Jan 01, 2010
Objective:
The objective of this cooperative research project is to develop an assay for the detection of pathogenic E. coli in water, soil, manure and food matrices using an integrated immuno-PCR biosensor.
Approach:
Pathogenic E. coli will be captured and fluorescently labeled with specific monoclonal antibodies. The E. coli cell-antibody complex will subsequently be detected using an immunoassay biosensor followed by PCR characterization.
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Last Modified: 01/14/2009
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