ARS | Publication request: Chitinase isoenzyme profiles in seedlings of Fusarium resistant and susceptible corn

Submitted to: Multicrop Aflatoxin and Fumonisin Elimination and Fungal Genomics Workshop-The Peanut Foundation
Publication Type: Abstract
Publication Acceptance Date: October 24, 2007
Publication Date: October 24, 2007
Citation: Naumann, T.A., Kendra, D.F. 2007. Chitinase isoenzyme profiles in seedlings of Fusarium resistant and susceptible corn [abstract]. Multicrop Aflatoxin and Fumonisin Elimination and Fungal Genomics Workshop-The Peanut Foundation. p. 3.

Technical Abstract: Plant chitinases have been implicated in both antagonistic and beneficial interactions with microorganisms. In an effort to better understand communication between Zea mays and its fungal environment, we are developing a knowledge base of corn chitinase isoenzymes. Of specific interest is the identification of proteins whose activity levels are affected by the presence of Fusarium. We have established isoenzyme profiles of imbibed seeds and seedlings for five lines of corn: the Fusarium resistant hybrid Enerfeast and its inbred parents, the genetic model B73, and the Fusarium sensitive sweet corn Jubilee. Twelve unique enzymes were identified that are able to degrade chitin after electrophoresis. The enzyme profiles were further characterized in Enerfeast by dissection of young seedlings into six separate tissues prior to protein extraction. Four chitinases have been purified and their isoelectric point and substrate profiles have been determined.