ARS | Publication request: Enhancement of mycotoxin fluorescence with cyclodextrins, and analytical applications

Submitted to: Association Official Analytical Chemists Annual Intrl Meeting & Exposition
Publication Type: Abstract
Publication Acceptance Date: September 16, 2007
Publication Date: September 16, 2007
Citation: Maragos, C.M., Appell, M.D., Catucci, L., Lilppolis, V., Visconti, A., Pascale, M. 2007. Enhancement of mycotoxin fluorescence with cyclodextrins, and analytical applications [abstract]. Association Official Analytical Chemists Annual Intrl Meeting & Exposition. Paper No. S-604.

Technical Abstract: Certain of the mycotoxins are known to form host-guest complexes with cyclodextrins (CDs), cyclic oligosaccharides containing a relatively hydrophobic pore. The interactions between mycotoxins and CDs can alter the properties of the mycotoxin, namely the fluorescence, absorption, or chromatographic properties of these fungal metabolites. In particular, the native fluorescence of aflatoxins, ochratoxin A, and zearalenone can be enhanced under certain conditions with CDs. Practical applications of this effect have been the incorporation of CDs into the mobile phase of capillary electrophoresis-based separations of aflatoxins, and zearalenone, and the use of CDs as reagents for improved aflatoxin detection in HPLC. Cyclodextrins can also be used to enhance the emission from fluorescently-labeled mycotoxins, such as T-2 toxin. Because CDs can affect aspects of the chromatography and detection of mycotoxins, they may make useful additives for a variety of analytical methods, especially those conducted under aqueous conditions.