ARS | Publication request: EXTRACTION AND CHARACTERIZATION OF ANTHOCYANINS FROM PIGMENTED FLOWERS AND BLACK SEED HULLS

Submitted to: Phytochemical Society of North America Meeting and Newsletter
Publication Type: Abstract
Publication Acceptance Date: July 12, 2006
Publication Date: July 12, 2006
Citation: Berhow, M.A., Johnson, E.T., Dowd, P.F., Duval, S.M. 2006. Extraction and characterization of anthocyanins from pigmented flowers and black seed hulls [abstract]. Phytochemical Society of North America Meeting and Newsletter. p. 22

Technical Abstract: New methodology has been developed for the preparative scale extraction and purification of anthocyanins from "black" seed coats and hulls. A combination of physical fractionation, extraction, flash chromatography and preparative chromatography has resulted in the isolation of gram quantities of purified anthocyanins from petunia and gentium flowers, and black soybeans, black beans and cuphea seed hulls. The anthocyanin structures were determined by LC-MS. The methodology developed can be used to quantitate the levels of the anthocyanins in various cultivars and growing conditions. The quantities being isolated allow for the evaluation of their insecticidal, fungicidal and herbicidal activities in a variety of bioassay systems.