Cell-Free Protein Synthesis Directed by Messenger RNA
Description:
Nirenberg's work with synthetic RNA and E. coli provided the basis for his genetic research in the early 1960s. This article describes the preparation of reagents, procedures for reaction mixtures, methods for growth of E. coli, and preparation of extracts. Their experiments ultimately proved that messenger RNA, which transcribes genetic information from DNA, directs protein synthesis. That is, messenger RNA transmits the DNA messages that prescribe the assembly of amino acids into the complex proteins that drive living processes.
Item is a photocopy.
Number of Image Pages:
7 (610,010 Bytes)
Date:
1964
Creator:
Nirenberg, Marshall W.
Source:
Periodical: Nirenberg, Marshall W. "Cell-Free Protein Synthesis Directed by Messenger RNA." Methods in Enzymology VI, (1964): 17-23. Article. 7 Images.
Publisher:
Academic Press
Rights:
Reproduced with permission of Academic Press.
Subject:
Medical Subject Headings (MeSH):
Amino Acids
RNA, Messenger
Enzymes
Exhibit Category:
Translating the Code of Life and the Nobel Prize, 1962-1968
