What is e-PCR
e-PCR identifies sequence tagged sites(STSs)within DNA sequences.
Using e-PCR, you can search for sub-sequences that closely match the PCR primers and have the correct order,
orientation, and spacing.
What's new
Improved Search Sensitivity
You can use multiple discontigous words instead of a single exact word.
Each of this word
has groups of significant positions seperated by 'wildcard' positions. It is not required that these positions
match. Also, it is now possible to allow gaps in the
primer alignments. A fuzzy matching strategy reduces the
likelihood that a true STS will be missed due to mismatches.
Reverse Searching
Searching the human genome sequence and
other large genomes is now possible. The new version of e-PCR provides a search mode using a
query sequence against a sequence database.
Pubmed References
Sequence mapping by electronic PCR.Schuler,GD. (1997)
A
web server for performing electronic PCR.Rotmistrovsky K, Jang W, Schuler GD. (2004)
