Fire Island National Seashore has formed partnerships with a variety of organizations in order to help the National Park Service fulfill its mission.

Fire Island National Seashore's primary partners include:

• Fire Island Lighthouse Preservation Society (FILPS)
  Formed in 1982 in response to the possible demolition of the Fire Island Lighthouse, the Society raised over $1.2 million to save and restore the Lighthouse. In 1996, the nonprofit group signed a 10-year Memorandum of Agreement with the National Park Service to maintain and operate the Fire Island Lighthouse. In 2006, FILPS signed a Cooperating Association Agreement to continue its support through a program of visitor services, publications sales, membership and fundraising activities.
  
  
• Eastern National
  This cooperating association maintains book sales outlets at Watch Hill, Sailors Haven, Wilderness visitor centers and at the William Floyd Estate.
  
  
• Friends of Fire Island National Seashore (FFINS)
  A nonprofit organization whose mission is to encourage proper stewardship of park natural resources, foster cooperative and mutual communication between the National Park Service, communities and user groups, and to promote environmentally sound access to all of the various aspects of Fire Island National Seashore.
  
• Friends of Watch Hill (FOWH)
  A non-profit organization whose mission is to promote and support the recreational use of Watch Hill, Fire Island while preserving its natural resources and beauty.
  
• Davis Park Ferry Company 
  An authorized park concessioner, this company operates the ferry service from Patchogue to Watch Hill and Barrett Beach.
  
• Sayville Ferry Service
  An authorized park concessioner, this company operates the ferry service from Sayville to Sailors Haven/Sunken Forest. 
  
• Fire Island Concessions, LLC
  An authorized park concessioner, Fire Island Concessions assumed the management of the Seashore's 2 marinas, snack bars, and the campground, camp store and restaurant in 2005.