Project Number: 5302-22000-008-32
Project Type: Trust

Start Date: Apr 06, 2009
End Date: Oct 31, 2011

Objective:
1. Perform genome-wide sequence analysis to identify Simple Sequence Repeat (SSR) loci. 2. Genotyping and phenotyping of CLas strains, characterizing virulent strains, host pathogen interactions; adaptation; selection and strain, strain competition. 3. Analyzing CLas population structures, assessing genetic diversity of CLas in Florida populations.

Approach:
1. Complete genome gaps from current draft genome using long distance PCR and genomic walking techniques. 2. PCR and fragment analyses of asiaticus isolates collected from Florida populations. 3. Conduct molecular variance analysis to assess hierarchical structures of pathogen population in Florida. Documents Reimbursable with FL Citrus Production Research Advisory Counsil. Log 37525.